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Decarboxamidation grain protein-chrominium element chelate and preparation method

A protein and deamidation technology, applied in the fields of plant peptides, food science, climate change adaptation, etc., can solve the problems of excessive chromium excretion, adverse health effects, and high biological toxicity, achieving low production costs, reducing environmental residues, The effect of improving the absorption rate

Inactive Publication Date: 2005-12-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chromium has three valence states of +6, +3 and +2 in nature, among which CR 6+ High biological toxicity, Cr 2+ Not stable enough, not suitable for chromium supplementation in the crowd
Trivalent inorganic chromium is cheap, safe, and soluble, but the absorption rate in the body is low, only 0.4-3% or even lower
Long-term consumption of refined foods will cause excessive excretion of chromium stored in the body, which will have adverse effects on health and even cause diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The preparation of deamidated cereal protein-chromium chelate is carried out in two steps. In the first step, hydrochloric acid is added to the cereal protein solution or suspension, and the cereal protein undergoes high-pressure denaturation and deamidation reaction under high temperature and high pressure conditions to make the protein Asparagine and glutamine deamidated to generate aspartic acid and glutamic acid, thereby increasing the charge density of protein macromolecules and improving solubility. Deamidated cereal proteins have a large number of carboxyl groups that can chelate metal ions. The addition method and dosage of hydrochloric acid are not very strict. Generally speaking, an appropriate amount of dilute hydrochloric acid can be poured directly under stirring conditions, or concentrated hydrochloric acid can be gradually added dropwise to control the pH value at 1-4. This invention recommends The pH should be controlled around 2.

[0028] In the second...

Embodiment 1

[0037] The preparation method of preparing deamidated wheat protein-chromium element chelate with gluten and chromium trichloride as raw material comprises the following steps:

[0038] 1) Take by weighing 20g gluten powder and place in a beaker, make a 5% suspension in deionized water, add concentrated hydrochloric acid to acidify the suspension while stirring electromagnetically, adjust the pH value of the suspension to 1, place the suspension in In a pressure cooker, deamidation reaction was carried out at 120°C and 0.11MPa for 5 minutes, and cooled to room temperature;

[0039] 2) While stirring, adjust the pH of the deamidated wheat protein suspension to pH=4.5 with a saturated NaOH solution, then perform centrifugation at 3000 g for 10 min, and pour off the supernatant to obtain a deamidated wheat protein solid;

[0040] 3) Disperse the deamidated wheat protein solid in 400ml of deionized water, extract the water-soluble deamidated wheat protein at 50°C under high-speed ...

Embodiment 2

[0043] The preparation method of deamidated rice protein powder-chromium element chelate is prepared by using rice protein powder and chromium nitrate as raw materials, comprising the following steps:

[0044] 1) Weigh 20g rice protein powder and place it in a beaker, make a 15% suspension with deionized water, add concentrated hydrochloric acid while stirring electromagnetically, adjust the pH value of the suspension to 3, and place the suspension in a high-pressure reactor Internal heating, deamidation reaction at 126°C and 0.15MPa for 30 minutes, and cool to room temperature;

[0045] 2) While stirring, wash with saturated NaHCO 3 The solution adjusts the pH value of the deamidated rice protein suspension to pH=4.2, and uses a funnel to filter and separate to obtain a deamidated rice protein solid;

[0046] 3) Disperse the deamidated rice protein solid in 400ml of deionized water, extract the water-soluble deamidated rice protein at 90°C under high-speed stirring, centrifu...

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Abstract

This invention has disclosed a manufacturing method to a chelate of decarboxamidation corny protein-chrome. Count by weight per cent, chromium content of chelate is 0.5 to 1.5 percent; This method is utilizing chemical activity of chemical groups of decarboxamidation corny protein-chrome, compound protein with chromium, make decarboxamidation corny protein-chrome including active Ce3 + ion, by centrifugal separation, filtrate or spray drying to get solid state powder.The technological process of this method are simple, the production cost is cheap, apt to popularize implementation.

Description

technical field [0001] The invention relates to a deamidated cereal protein-chromium element chelate and a preparation method thereof. Background technique [0002] Chromium is one of the essential trace elements for the human body, and its contribution to the physiological functions of the human body is mainly manifested in the following aspects: [0003] 1)Cr 3+ Ions are part of the active center of glucose tolerance factor (GIF) and play important physiological functions in the human body. Cr 3+ Ions can assist insulin to maintain normal glucose tolerance, increase the synthesis rate of glycogen in the body, and increase the mobility of blood sugar, so that glucose can be quickly transferred from the liver to other tissues and used. At the same time, Cr 3+ Ions cooperate with insulin to participate in the metabolism of lipids, proteins and nucleic acids, improve blood lipid status, and have significant lipid-lowering and cholesterol-lowering effects. [0004] 2) Chro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/165A23L33/185C07K14/415
CPCY02A40/90
Inventor 宋义虎郑强叶瑛
Owner ZHEJIANG UNIV
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