Method for preparing distillers' grain protein feed through three-stage mixed bacterium solid state fermentation

A distiller's grain protein feed and solid-state fermentation technology, applied in animal feed, animal feed, applications, etc., can solve the problems of crude protein content, low digestible energy and metabolizable energy, low added value of feed, and affecting the effective utilization of nutrients, etc.

Active Publication Date: 2014-08-27
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of distiller's grain feed processing, most domestic enterprises directly use solid liquor distiller's grains as livestock and poultry feed or dry distiller's grains to make granular feed for sale. The added value of fee

Method used

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  • Method for preparing distillers' grain protein feed through three-stage mixed bacterium solid state fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0062] (1) The first stage of solid-state fermentation with mixed bacteria: use 100kg of fresh discarded grains as the substrate, adjust the pH value to 6.5 with lime, add 10kg of group A Bacillus combined seed bacterial liquid, mix evenly and put it into sacks, stack fermentation and naturally heat up to 63°C Finally, pour out the sacks and spread the production site to cool down to 45°C, then accumulate and ferment and raise the temperature to 60°C, maintain the fermentation temperature between 55°C and 60°C by turning over, stacking, and spread out, the fermentation time is 2 days, and the product is distiller's grains. .

[0063] (2) The second stage of solid-state fermentation with mixed bacteria: add 16kg of pagoda tree leaf powder, 11.1kg of poplar leaf powder by weight, 11kg of bran, and 0.16kg of dipotassium hydrogen phosphate to the 110kg of distiller's grains obtained after the first stage of fermentation , 0.11kg magnesium sulfate, 2.2kg ammonium sulfate, at the sa...

Embodiment 2

[0068] (1) The first stage of solid-state fermentation with mixed bacteria: use 100kg of fresh discarded grains as the substrate, adjust the pH value to 6.5 with lime, add 10kg of group A Bacillus combined seed bacteria liquid, mix evenly and put it into sacks, pile up and ferment and naturally heat up to 65°C Finally, pour out the sacks and spread the production site to lower the temperature by 45°C, then pile up and ferment and raise the temperature to 60°C, maintain the fermentation temperature between 55°C and 60°C by turning over, stacking, and spread out, and the fermentation time is 3 days. The product is distiller's grains.

[0069] (2) The second stage of solid-state fermentation with mixed bacteria: Weigh 100kg of distiller's grains fermented grains after the first stage of fermentation, add 18kg of pagoda tree leaf powder, 10kg of poplar leaf powder, 12kg of bran, 0.15kg of dipotassium hydrogen phosphate, and 0.1kg of sulfuric acid Magnesium, 2kg of ammonium sulfate,...

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Abstract

The invention belongs to the technical field of microbial fermentation, and concretely relates to a method for preparing a distillers' grain protein feed through three-stage mixed bacterium solid state fermentation. The method comprises the following steps: 1, first stage fermentation: carrying out high temperature fermentation on an A group bacillus combined seed liquid at 60DEG C with distillers' grains as a substrate to obtain a distillers' grain fermentation material; 2, adding locust tree leaf powder, poplar tree leaf powder and wheat bran to the distillers' grain fermentation material obtained after the first stage fermentation, inoculating a Geotrichum candidum, yeast and Trichoderma spp. seed liquid, and blending to obtain a fermentation material, and carrying out second stage fermentation; and 3, inoculating a final product obtained after the second stage fermentation to a B group bacillus combined seed liquid, carrying out third stage fermentation to obtain a final fermentation product fresh distillers' grain protein feed, or drying, crushing, granulating and packaging to obtain a particulate distillers' grain protein feed. The content of crude proteins in the distillers' grain protein feed prepared by adopting the method is 29-32% averagely, and is 58-74% higher than the content of crude proteins in dry distillers' grains.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation. It specifically relates to a three-stage solid-state fermentation of solid liquor distillers grains [0002] A method for preparing distiller's grain protein feed by fermentation. Background technique [0003] Solid distiller's grains are a by-product of the solid-state liquor brewing industry. According to statistics, the annual production of distiller's grains in my country reaches 21 million tons, and the quantity is large and concentrated. If it is not processed in time, the distiller's grains are prone to corruption, which not only causes a large waste of resources, but also seriously pollutes the surrounding environment. Therefore, the comprehensive utilization of distiller's grains is of great significance to the resource development and environmental protection of our country. [0004] At present, my country has made some achievements in the research on the comprehens...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14A23K1/06A23K10/10A23K10/38
CPCY02P60/87
Inventor 黄祖新黄镇赖玉城杨玉强
Owner FUJIAN NORMAL UNIV
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