Economical method for quickly screening high-protein variety of paddy rice

Abstract

The invention belongs to the fields of genetic breeding and innovation of germplasm resources, and provides an economical novel method for quickly screening high-protein variety of paddy rice. The method comprises the following steps of: comparing by taking the paddy rice variety which is tested by a Kjeldahl method to have the protein content of about 8-9% as medium-protein content, randomly selecting one grain of polished rice from each variety to be tested, dipping in distilled water in a 1.5ml small centrifuge tube for 3 hours, breaking the polished rice to be naturally fractured by hands, dyeing by acridine orange (0.02%, v / v) for 10 minutes under the condition of keeping out of the sun, and washing by the distilled water for 1 minute. The Nikon ECLIPSE 90i laser scanning confocal microscope is used for observing, excitation light is 488nm, emitted light is 575nm-605nm, and NIH Image 1.6.2 software is used for analyzing an image. The transverse facial cleft of the polished rice is higher in protein content compared with the coarse surface, the higher the roughness is, the higher the protein content is, and otherwise the protein content is lower. The method can be used for quickly screening the high-protein variety of the paddy rice by few samples for the subsequent genetic breeding, so that the quality of the paddy rice can be improved.

Description

Technical field [0001] The patent of the present invention relates to the field of genetic breeding of rice by comparing cross-sections of one rice seed to screen high-protein varieties, and is a fast and economical method for screening high-protein varieties of rice. Background technique [0002] At present, the method for analyzing the protein content of seeds, the first step is to extract the protein in the seeds, and then detect the protein content, commonly used Kjeldahl method, biuret method, Coomassie brilliant blue method, ultraviolet absorption method. The experimental process requires many seed samples and many reagents, which is time-consuming and labor-intensive. The operation of measuring protein content by near-infrared spectroscopy is also relatively simple, but only 5g (about 250 grains) of samples are required to obtain good results. [0003] The active group of the dye acridine orange is stably combined with the hydroxyl group of the starch through electro...

AI Technical Summary

Problems solved by technology

The experimental process requires a lot of seed samples and prepared reagents, which is time-consuming and labor-intensive