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Rapid protein detection kit as well as detection method and application using same

A detection kit and detection method technology, applied in the direction of analysis by chemical reaction of materials, material analysis by observing the influence of chemical indicators, etc., can solve the problems of poor detection sensitivity and difficulty, and achieve high sensitivity, The effect of less interference and simple operation

Inactive Publication Date: 2016-04-13
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some serious defects in biuret spectrophotometry. Although the detection time of this method is short (20-30 minutes), the detection sensitivity is poor, and it is difficult to measure samples with low protein content.

Method used

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  • Rapid protein detection kit as well as detection method and application using same
  • Rapid protein detection kit as well as detection method and application using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The detection kit of the present invention is used to prepare a Tween 80 microemulsion system-biuret chromogenic solution to measure the sample protein.

[0022] (1) Preparation of Tween 80 microemulsion system solution

[0023] ① Preparation of mixed surfactant solution

[0024] Accurately weigh 0.25g of Tween 80 nonionic surfactant and 0.25g of absolute ethanol and mix them to make a mixed surfactant.

[0025] ②Preparation of mixed solution of mixed surfactant and oil phase

[0026] Ethyl butyrate was selected as the oil phase of the emulsion system, and 0.05 g of ethyl butyrate was added to the above mixed surfactant.

[0027] ③ Preparation of mixed surfactant / oil phase and water phase microemulsion system

[0028] Stir the mixture of Tween 80, absolute ethanol and ethyl butyrate at room temperature, add deionized water dropwise while stirring, stop adding deionized water when the mixture changes from turbid to clear and transparent solution.

[0029] (2) Prepara...

Embodiment 2

[0036] Using the detection kit of the present invention, TritonX-100 (polyethylene glycol octyl phenyl ether, non-ionic) microemulsion system-biuret chromogenic solution is prepared, and the sample protein is measured.

[0037] (1) Preparation of TritonX-100 microemulsion system solution

[0038] ① Preparation of mixed surfactant solution

[0039] Accurately weigh 0.75g TritonX-100 surfactant and 0.25g n-propanol and mix them to make mixed surfactant.

[0040] ②Preparation of mixed solution of mixed surfactant and oil phase

[0041] Ethyl butyrate was selected as the oil phase of the microemulsion system, and 0.1 g of ethyl butyrate was added to the above mixed surfactant.

[0042] ③ Preparation of mixed surfactant / oil phase and water phase microemulsion system

[0043] Stir the above-mentioned mixture of TritonX-100, n-propanol and ethyl butyrate at room temperature, add deionized water dropwise while stirring, and stop adding deionized water when the mixed solution change...

Embodiment 3

[0051] The detection kit of the present invention is used to prepare a microemulsion system-biuret chromogenic solution to measure the sample protein.

[0052] (1) Preparation of microemulsion system solution

[0053] ① Preparation of mixed surfactant solution

[0054] Accurately weigh 25g of SDS (sodium dodecylbenzenesulfonate) or Tween 60 and 0.25g of n-butanol, and mix them to make a mixed surfactant.

[0055] ②Preparation of mixed solution of mixed surfactant and oil phase

[0056] Ethyl butyrate was selected as the oil phase of the emulsion system, and 252.5 g of ethyl butyrate was added to the mixed surfactant.

[0057] ③ Preparation of mixed surfactant / oil phase and water phase microemulsion system

[0058] Stir the mixture of the above SDS or Tween 60, n-butanol and ethyl butyrate at room temperature, add deionized water dropwise while stirring, stop adding deionized water when the mixed solution changes from turbid to clear and transparent solution .

[0059] (2)...

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Abstract

The invention discloses a rapid protein detection kit as well as a detection method and an application using the same. The detection kit comprises a biuret reagent, a surfactant and a cosurfactant. According to the detection method disclosed by the invention, through addition of the surfactant and the cosurfactant in the biuret reagent, the prepared microemulsion has the characteristics of solubilization and sensibilization, the improved biuret method has the advantages of high sensitivity, simplicity in operation, less interferents and good repeatability. The detection sensitivity of the detection method disclosed by the invention on protein is 6-11 times that of the traditional method. The detection method can be applied to detection of a trace protein sample, and the relative error of the detection method is between 0.28% and 0.60% as compared with that of an national standard kjeldahl determination method. The detection method disclosed by the invention has good precision and accuracy.

Description

technical field [0001] The invention belongs to the technical field of biochemical detection, and in particular relates to a protein rapid detection kit and a detection method and application thereof. Background technique [0002] Protein is the material basis of all life and the main raw material for the renewal and repair of human tissue. Protein is closely related to various forms of life activities and has extremely important physiological significance. At the same time, protein is one of the important nutrients. Protein in food is the only source of nitrogen intake for the human body and has an irreplaceable role for other nutrients. In recent years, incidents of protein adulteration in food have occurred from time to time, causing serious harm to the human body. Therefore, it is imminent to establish a fast, accurate, and easy-to-operate protein detection method. [0003] At present, the authoritative method for food protein detection is the Kjeldahl method, which is ...

Claims

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Application Information

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IPC IPC(8): G01N21/79
CPCG01N21/79
Inventor 田波马丽华田然刘平平陈晨高宝玉李野
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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