Method for measuring crude protein content in Phalaris arundinacea by using near infrared spectrum

Abstract

The invention relates to the field of measurement of pasture quality, and in particular relates to a method for measuring crude protein content in Phalaris arundinacea by using near infrared spectrum.The method comprises the following steps of 1, widely collecting representative Phalaris arundinacea samples; 2, collecting near infrared spectrum data of modeled samples; 3, measuring the crude protein content in the samples by using a kjeldahl determination method; 4, building a calibration model; 5 verifying the calibration model; and 6, measuring the Phalaris arundinacea samples to be measured. The measurement method is easy to acquire samples and convenient to measure; continuous and unlimited analysis can be performed after the model is built, the time for being on the machine only needs 16s, the test period is shortened, the analysis efficiency is greatly improved, the measurement result is good in reproducibility and high in stability, meanwhile no chemical reagent is used, no pollution and damage are generated. The method can be used for quickly measuring the plenty of material, and has the wide application prospect in the aspects of Phalaris arundinacea quality assessment, quality breeding, daily ration configuration and forage product circulation.

Description

technical field [0001] The invention relates to the field of forage quality measurement, in particular to a method for detecting the crude protein content of Lilygrass by using near-infrared spectroscopy. Background technique [0002] Grass is a cool-season herbaceous perennial grass of the Gramineae family. The plant is tall, leafy, and high-yielding. It has excellent agronomic properties and high nutritional value. It has a strong root system and well-developed rhizomes. It is widely distributed in temperate regions such as North America, Northern Europe and Asia, and is also widely distributed in my country. Grass grass grows rapidly, has strong regeneration and high grass yield. The yield of fresh grass per mu is 3500-4000kg, and the yield of hay per mu is 800-1000kg. Well, livestock like horses, cattle and sheep like to eat. Therefore, it is widely used in artificial grassland construction, grassland ecological protection, grassland improvement, etc., and is of great s...

AI Technical Summary

Problems solved by technology

At present, there are two main methods for determining the crude protein content of Glycerus chinensis. One is the Kjeldahl method (Kjeldahl method) which is the most classic method for determining the crude protein content. It has a history of more than 100 years, but the Kjeldahl method The measurement cycle is long (>5h), and the chemical reagents used (such as concentrated sulfuric acid, sodium hydroxide solution, etc.) have potential hazards to the experimenter's body and are likely to cause environmental pollution; the second is the Dumas combustion method (Dumas method ) measurement speed is fast (about 10min), the domestic promotion rate is low, the use is less, the consumables are expensive, and there are restrictions on the determination of the protein form and content of the sample

It can be seen that the chemical determination methods currently used have disadvantages such as time-consuming, laborious, high cost, cumbersome steps, and environmental pollution. Therefore, there is an urgent need for a rapid, efficient, accurate, safe, low-cost, and wide-ranging method for the determination of crude protein in Asparagus

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