Preparation method of lycium barbarum polysaccharide

A technology of Lycium barbarum polysaccharide and Lycium barbarum, which is applied in the field of biological extraction and separation, can solve the problems of polysaccharide degradation, polysaccharide biological activity destruction, short extraction time, long time consumption, etc., and achieves the effects of improving extraction rate, short extraction time and high efficiency.

Active Publication Date: 2014-05-14
百瑞源枸杞股份有限公司 +1
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of long time-consuming, high energy consumption, low yield, high-temperature degradation of polysaccharides and destruction of polysaccharide biological activity caused by traditional ho

Method used

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  • Preparation method of lycium barbarum polysaccharide
  • Preparation method of lycium barbarum polysaccharide
  • Preparation method of lycium barbarum polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 A kind of preparation method of Lycium barbarum polysaccharide

[0035] (1) Soak the wolfberry with water (1:10), beat the pulp, and separate the pulp from the wolfberry seeds. You can use stirring to naturally settle the wolfberry seeds, or use an automatic seed removal machine to remove the wolfberry seeds;

[0036] (2) Lycium barbarum pulp was extracted in a circulating ultrasonic extraction machine; the extraction conditions were: temperature 40°C, ultrasonic power 500W, circulation speed 2000rpm; duty ratio 1:3, ultrasonic extraction time 2h.

[0037] (3) After the extraction is completed, the solid and liquid are separated, and the supernatant is concentrated under vacuum and reduced pressure;

[0038] (4) After concentrating to 1 / 10 of the volume of the original extract, add ethanol until the concentration of ethanol is 75% for static precipitation to obtain the crude polysaccharide precipitate of Lycium barbarum.

[0039] (5) Dry the crude polysacc...

Embodiment 2

[0040] Embodiment 2 A kind of preparation method of Lycium barbarum polysaccharide

[0041] (1) Soak wolfberry with water (material-to-liquid ratio 1:8), beat, stir and suspend the pulp, and then let it stand for 10 minutes to allow the wolfberry seeds to settle naturally, thereby separating the wolfberry seeds;

[0042] (2) Put wolfberry pulp in a circulating ultrasonic extraction machine for extraction. The conditions of circulating ultrasonic extraction are: temperature 30°C, ultrasonic power 1200W, cycle speed 1000rpm, duty ratio 1:2, ultrasonic extraction time 1h.

[0043] (3) After the extraction is completed, the solid and liquid are separated, and the supernatant is concentrated by vacuum decompression, and the concentration ratio is 1 / 5 of the volume of the extract.

[0044] (4) After concentrating the extract, add ethanol until the concentration of ethanol is 85%, and let it stand for precipitation to obtain crude polysaccharide precipitate of Lycium barbarum.

[00...

Embodiment 3

[0054] Embodiment 3 A kind of preparation method of Lycium barbarum polysaccharide

[0055] (1) Soak wolfberry with water (material-to-liquid ratio: 1:20), make a pulp, and use an automatic seed separator to separate the seeds of wolfberry.

[0056] (2) Lycium barbarum pulp was extracted in a circulating ultrasonic extraction machine; the conditions of circulating ultrasonic extraction were: temperature 30°C, ultrasonic power 800W, cycle speed 1500rpm, duty ratio 1:2, ultrasonic extraction time 1.5h.

[0057] (3) After the extraction is completed, the solid and liquid are separated, and the supernatant is concentrated by vacuum concentration under reduced pressure;

[0058] (4) After the extract was concentrated, ethanol was added to a final concentration of 80% for static precipitation to obtain crude polysaccharide precipitates from Lycium barbarum.

[0059] (5) The crude polysaccharide precipitate is dried by spray drying. The spray drying conditions are: the air inlet tem...

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Abstract

The invention relates to a preparation method of lycium barbarum polysaccharide. The preparation method comprises the following steps: adding water to soak lycium barbarum; pulping; removing seeds; placing lycium barbarum pulp in a circular ultrasonic extraction machine for ultrasonic extraction; carrying out solid-liquid separation; concentrating the filtrate; adding a certain amount of ethanol to precipitate polysaccharide; purifying the polysaccharide by resin to prepare a lycium barbarum polysaccharide product. The preparation method provided by the invention solves the problems that conventional extraction of lycium barbarum polysaccharide by hot water is long in time consumption, high in energy consumption and low in yield, degradation to polysaccharide by high temperature, the biological activity of polysaccharide is destroyed and the like by means of advantages of a circular ultrasonic extraction technology so as to realize the purpose of quickly and efficiently producing lycium barbarum polysaccharide.

Description

technical field [0001] The invention belongs to the field of biological extraction and separation of plant natural products, in particular to a preparation method of Lycium barbarum polysaccharide. Background technique [0002] Lycium barbarum (Lycium barbarum) is not only a traditional Chinese herbal medicine but also a common food ingredient in the folk. It has various pharmacological effects and bioactive functions. According to modern medical clinical research, Lycium barbarum polysaccharides (LBP) extracted from Lycium barbarum is one of the main active ingredients of Lycium barbarum biological function, and it has become an important functional additive of health food. In recent years, domestic research on polysaccharides has progressed rapidly. The extraction and separation, chemical analysis, pharmacological effects and clinical application of Lycium barbarum polysaccharides have a relatively extensive research basis and show good application prospects. [0003] The...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 赵兵赵庆生郝向峰张金宏王玉玲
Owner 百瑞源枸杞股份有限公司
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