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Preparation method of RG-I type lycium barbarum pectin with anti-aging activity

A technology of RG-I and Lycium barbarum pectin, applied in the direction of anti-toxic agents, drug combinations, etc., can solve the problem of lack of isolation and purification methods of Lycium barbarum polysaccharide antioxidant active centers, difficulty in anti-oxidative ability of Lycium barbarum polysaccharides, and failure to find antioxidant active centers, etc. problem, achieve good anti-aging ability, significant anti-aging activity, and improve the effect of anti-oxidation ability

Active Publication Date: 2015-10-21
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the preparation methods for improving the antioxidant activity of Lycium barbarum polysaccharides are only aimed at the polysaccharide extraction and impurity removal process, and do not involve further separation and purification of active polysaccharide components. Therefore, it is difficult to fundamentally improve the antioxidant activity of Lycium barbarum polysaccharides Antioxidant capacity
However, up to now, there are no patents and reports on the antioxidant active center of Lycium barbarum polysaccharide and its separation and purification method

Method used

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  • Preparation method of RG-I type lycium barbarum pectin with anti-aging activity
  • Preparation method of RG-I type lycium barbarum pectin with anti-aging activity
  • Preparation method of RG-I type lycium barbarum pectin with anti-aging activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1 A kind of preparation method of RG-I type wolfberry pectin with anti-aging activity comprises the following steps:

[0072] (1) add water to Lycium barbarum through microwave-ultrasonic synergistic extraction to obtain an extract, which is filtered to remove the precipitate, and the filtrate is collected; the filtrate is concentrated to 1 / 6 of the volume of the filtrate at a temperature of 60°C to obtain a concentrate .

[0073] Among them: wolfberry is Ningxia wolfberry ( Lycium barbarum L. ) of ripe fruit.

[0074] The microwave-ultrasonic collaborative extraction conditions refer to the solid-liquid mass-volume ratio (g / mL) of 1:10, the extraction temperature of 60°C, the ultrasonic power of 600W, the microwave power of 340W, and the total extraction time of 20min.

[0075] (2) Add anhydrous ethanol three times its volume to the concentrated solution, mix well, let stand at 4°C for 12 hours, and centrifuge to obtain the precipitated product.

[0076]...

Embodiment 2

[0092] Embodiment 2 A preparation method of RG-I type wolfberry pectin with anti-aging activity, comprising the following steps:

[0093] (1) add water to Lycium barbarum and extract through microwave-ultrasonic synergy to obtain an extract, which is filtered to remove precipitates, and the filtrate is collected; the filtrate is concentrated to 1 / 8 of the volume of the filtrate at a temperature of 80°C to obtain a concentrate .

[0094] Among them: wolfberry is Ningxia wolfberry ( Lycium barbarum L. ) of ripe fruit.

[0095] The microwave-ultrasonic collaborative extraction conditions refer to the solid-liquid mass-volume ratio (g / mL) of 1:20, the extraction temperature of 100°C, the ultrasonic power of 800W, the microwave power of 380W, and the total extraction time of 30min.

[0096](2) Add 4 times the volume of absolute ethanol to the concentrated solution, mix well, let stand at 10°C for 24 hours, and centrifuge to obtain the precipitated product.

[0097] Wherein: the...

Embodiment 3

[0113] Embodiment 3 A preparation method of RG-I type wolfberry pectin with anti-aging activity, comprising the following steps:

[0114] (1) add water to Lycium barbarum and extract through microwave-ultrasonic synergy to obtain an extract, which is filtered to remove precipitates, and the filtrate is collected; the filtrate is concentrated to 1 / 7 of the volume of the filtrate at a temperature of 70°C to obtain a concentrate .

[0115] Among them: wolfberry is Ningxia wolfberry ( Lycium barbarum L. ) of ripe fruit.

[0116] The microwave-ultrasonic collaborative extraction conditions refer to the solid-liquid mass-volume ratio (g / mL) of 1:15, the extraction temperature of 80°C, the ultrasonic power of 700W, the microwave power of 360W, and the total extraction time of 25min.

[0117] ⑵ Add 3.5 times the volume of absolute ethanol to the concentrated solution, mix well, let stand at 7°C for 18 hours, and centrifuge to obtain the precipitated product.

[0118] Wherein: the ...

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Abstract

The invention relates to a preparation method of RG-I type lycium barbarum pectin with anti-aging activity. The method comprises the following steps: (1) water is added into lycium barbarum, and the mixture is subjected to microwave-ultrasonic assisted extraction, filtering and concentration, such that a concentrate liquid is obtained; (2) anhydrous ethanol is added into the concentrate liquid, and the materials are well mixed; the mixture is allowed to stand and is subjected to centrifugation, such that a precipitation product is obtained; (3) the precipitation product is re-dissolved, and the solution is subjected to centrifugation and drying, such that lycium barbarum polysaccharide is obtained; (4) lycium barbarum polysaccharide subjected to DEAE-cellulose column chromatography separation, and is eluted; and dialysis desalination and lyophilization are carried out, such that lycium barbarum neutral polysaccharide LBP-N and lycium barbarum acidic polysaccharide LBP-A are respectively obtained; (5) lycium barbarum acidic polysaccharide LBP-A is subjected to Sepharose CL-6B gel filtration column chromatography separation, and is eluted; dialysis desalination and lyophilization are carried out, such that lycium barbarum acidic polysaccharides LBP-A-1 and LBP-A-2 are obtained; (6) lycium barbarum acidic polysaccharide LBP-A-1 is subjected to DEAE-Sepharpse Fast Flow anion exchange column chromatography purification; continuous gradient elution is carried out with a 0-0.5 mol / L NaCl aqueous solution; and dialysis desalination and lyophilization are carried out, such that the anti-aging RG-I type lycium barbarum pectin pure product is obtained. The method provided by the invention has the advantages of simple operation and high extraction rate

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of RG-I type wolfberry pectin with anti-aging activity. Background technique [0002] Lycium barbarum is the dry and mature fruit of Lycium barbarum, belonging to Solanaceae (Solanaceae), Solanaceae (Solanceae Reihb), Lyciinae Wettst (Lyciinae Wettst), Lycium barbarum ( Lycium L.), the most famous of which is Ningxia Lycium barbarum ( Lycium barbarum L.). Lycium barbarum is a traditional precious Chinese medicinal material in my country. It plays an important role in traditional medicine in China, and its medicinal value has been highly praised by physicians of all ages. [0003] Modern pharmacological studies have shown that the pharmacological and health effects of Lycium barbarum are related to the biologically active substance Lycium barbarum polysaccharide ( Lycium barbarum Polysaccharide, LBP) has a lot to do with it. It is the main active ingredien...

Claims

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Application Information

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IPC IPC(8): C08B37/06A61P39/06
Inventor 毕宏涛魏立新高婷婷杜玉枝杨红霞李岑
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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