Extraction process of lycium barbarum polysaccharide

An extraction process, Lycium barbarum polysaccharide technology, applied in the field of biological extraction and separation, can solve the problems of polysaccharide degradation, polysaccharide bioactivity destruction, low yield, and long time consumption, and achieve high extraction efficiency, short extraction time, and broad application prospects Effect

Inactive Publication Date: 2018-07-27
重庆市洲仨科技发展有限公司
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, the present invention proposes an extraction process of Lycium barbarum polysaccharides, which is used to solve the problems of long time consumption, high energy consumption, low yield, degradation of polysaccharides by high temperature and destruction of polysaccharide biological activity caused by traditional hot water extraction of Lycium barbarum polysaccharides. question

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  • Extraction process of lycium barbarum polysaccharide
  • Extraction process of lycium barbarum polysaccharide

Examples

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Effect test

Embodiment 1

[0039] Screen 100g of high-quality wolfberry fruits produced in Zhongning, Ningxia, and place them in vacuum-drying at 50°C for 24 hours, crush the dried wolfberry fruits through a coarse particle crusher, and then separate the pulp powder and wolfberry seeds through a three-stage sieve; crush and sieve at room temperature Conditions are carried out, the wolfberry powder is added to petroleum ether for extraction, the defatted powder is vacuum-dried at 60°C to constant weight; the defatted powder is added to a compound enzyme solution for extraction, the compound enzymes are cellulase and papain, and the compound enzyme configuration method is: 0.1mol / L citric acid and sodium citrate solution is configured as a Ph6 buffer solution, cellulase and papain are dissolved in the buffer to obtain a compound enzyme solution, and the degreasing powder is added to the compound enzyme solution for leaching, and the temperature must be lowered to 45°C in advance, and then leaching Incubat...

Embodiment 2

[0041] Screen 100g of high-quality wolfberry fruits produced in Zhongning, Ningxia, and place them in vacuum-drying at 50°C for 24 hours, crush the dried wolfberry fruits through a coarse particle crusher, and then separate the pulp powder and wolfberry seeds through a three-stage sieve; crush and sieve at room temperature Conditions are carried out, the wolfberry powder is added to petroleum ether for extraction, the defatted powder is vacuum-dried at 60°C to constant weight; the defatted powder is added to a compound enzyme solution for extraction, the compound enzymes are cellulase and papain, and the compound enzyme configuration method is: 0.1mol / L citric acid and sodium citrate solution is configured as a Ph8 buffer solution, cellulase and papain are dissolved in the buffer to obtain a compound enzyme solution, and the defatted powder is added to the compound enzyme solution for leaching, and the temperature must be lowered to 45°C in advance, and then leaching Keep warm...

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Abstract

The invention relates to the technical field of biological extraction and separation in plant natural products, in particular to an extraction process of lycium barbarum polysaccharide. The process comprises the following steps of (1) screening and impurity removal: screening lycium barbarum fruits; removing impurities and diseased fruits; (2) dewatering and drying: putting the lycium barbarum fruits at 50 DEG C to be subjected to vacuum drying for 24h; (3) crushing and separation: crushing the dried lycium barbarum fruits by a coarse particle crushing machine; then, passing the lycium barbarum fruits through a three-stage sieve to separate pulp powder and lycium barbarum seeds, wherein the crushing and the sieving are performed under normal temperature condition; (4) extraction and enzymolysis: adding petroleum ether into the lycium barbarum powder for extraction; performing vacuum drying on degreased powder at 60 DEG C to reach the constant weight; adding the degreased powder into acomplex enzyme solution for extracting; then, putting the degreased powder into a boiling water bath to be heated for enzyme deactivation; performing suction filtering after cooling; concentrating thesolution; (5) adding 4 times of volume of absolute ethyl alcohol into the concentrated solution; performing still standing for 12h at 4 DEG C for alcohol precipitation; performing centrifugation for15min under the condition of 4200rpm; taking precipitates; washing the precipitates by absolute ethyl alcohol for three times; performing drying to obtain coarse polysaccharide.

Description

technical field [0001] The invention relates to the technical field of biological extraction and separation of plant natural products, in particular to an extraction process of wolfberry polysaccharide. Background technique [0002] Lycium barbarum (Lycium barbarum) is not only a precious traditional Chinese medicinal material in my country but also a common food ingredient in the folk. It has various pharmacological effects and biologically active functions. Modern medical clinical research believes that Lycium barbarum polysaccharides (LBP) extracted from Lycium barbarum is one of the main active ingredients of Lycium barbarum biological effects, and it has become an important functional additive of health food. In recent years, domestic research on polysaccharides has progressed rapidly. The extraction and separation, chemical analysis, pharmacological effects and clinical application of Lycium barbarum polysaccharides have a relatively extensive research basis and show g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 孙一心
Owner 重庆市洲仨科技发展有限公司
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