Method for preparing oil tea saponin

A technology of camellia oleifera saponin and camellia oleifera oil, which is applied in the field of preparation of saponins, can solve the problems of low total saponin content in crude extracts, difficulty in processing, long extraction time, etc., and achieve protection of composition and structure, low cost and high purity high effect

Inactive Publication Date: 2008-09-24
DALIAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

There is also the water extraction method, but this method has more impurities, and the total saponin content in the crude extract is lower, and in order to improve the yield of the extract, it needs a long extraction time, which is time-consuming and labor-intensive; The existence of a large number of impurities such as protein brings great difficulty to the follow-up processing

Method used

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  • Method for preparing oil tea saponin
  • Method for preparing oil tea saponin
  • Method for preparing oil tea saponin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 22

[0033] This embodiment is an orthogonal experiment to investigate the extraction process. The extraction process steps are the same as those in the aforementioned Examples 1-21. According to the results of the aforementioned Examples 1-21, 3 appropriate levels are selected for the 4 factors respectively, and L 9 (3 4 ) Orthogonal experiment schedule to investigate the optimal process conditions. The horizontal arrangement of the factors in the orthogonal experiment is shown in Table 2, and the experimental results are shown in Table 3:

[0034] Table 1

[0035]

[0036] Table 2

[0037]

[0038] table 3

[0039]

[0040] It can be seen from the analysis that the influence of each factor is A>B>C>D, that is, the extraction solvent times the amount> extraction temperature> extraction time> extraction solvent concentration, so the best process combination is A 1 B 2 C 2 D. 2 , that is, 9 times the amount of 60% acetone-water 70 ° C extraction twice, each time for ...

Embodiment 23～30

[0047] Crush the camellia oleifera after oil extraction as raw material for extraction: the extraction solvent is acetone-water solution with a concentration of 60%, the ratio of solid to liquid (g:ml)=1:9, the extraction temperature is 70°C, the extraction time is 3h, and the extraction times are 2 times; the solution was concentrated and dried, then dissolved in water and filtered, and the filtrate was passed through D 101 After the macroporous resin is adsorbed, the impurities are eluted successively with distilled water and ethanol solution according to the set conditions, and the target product is eluted with the ethanol solution of the set concentration. In the examples of this part, the flow rate of the eluent is 2BV / h , enrich the eluate containing the target product, and pass through D at an equal elution rate 941 The macroporous resin column is decolorized, concentrated and dried to obtain the camellia saponin product, the content (%) is determined, and the yield is ...

Embodiment 31

[0051] The technical process of this embodiment is as attached figure 1 shown.

[0052] Carry out solvent extraction with the Camellia oleifera oilseed dregs after oil pressing, the extraction solvent is the acetone-water solution that concentration is 60%, solid-liquid ratio (g:ml)=1:9, extraction temperature 70 ℃, extraction time 3h, extraction The number of times is 2 times; after testing, the content of saponin in 60% of the acetone extract is 55%;

[0053] After the extract was concentrated and dried, it was dissolved in water, filtered, and made into a liquid medicine containing 118 mg / ml of total saponins. The loading amount was 3 times the resin weight, and the filtrate was passed through D 101 After the macroporous resin is adsorbed, it is eluted successively with 3BV of distilled water, 1BV of 20% ethanol, and 3BV of 85% ethanol. The last part of the eluate is collected and passed through D 941 The macroporous resin column is decolorized, concentrated and dried to ...

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Abstract

The present invention provides a method for preparing sasanqua saponin. According to a solid-liquid ratio of (g:ml) 1:6 to 14 and under the temperature between 60 DEG C to 100 DEG C, in the method, first 50 percent to 80 percent of acetone-aqueous solution is used to extract oil-extracted sasanqua oil meal or seeds for one to five times in a period of one hour to five hours, and after extractive is purified by D101 macroporous resin enrichment, decolorized by a D941 macroporous resin column, condensed and dried, a sasanqua saponin product is produced. The sasanqua saponin produced by the preparation method of the present invention is characterized in high purity, good quality and good colour and lustre; the method resolves the defects of using acid and alkali, enzyme, flocculating agent, ultrasonic processing and high-boiling normal butanol and water, effectively protecting the ingredient composition and structure of saponin; moreover, the preparation method has the advantages of simplicity and low cost, and acetone and is suitable for mass production, ethanol and macroporous resin can be recycled.

Description

technical field [0001] The invention relates to a method for extracting and preparing active ingredients in natural plant camellia oleifera, in particular to the preparation of saponins. Background technique [0002] Camellia oleifera Abel is a plant of the genus Camellia, which is mainly produced in my country and distributed in 17 provinces, concentrated in the southwest and south of my country. According to incomplete statistics, my country has an area of ​​3.36 million hectares of camellia oleifera, with an annual output of 645,000 tons of camellia oleifera seeds. There are 6 million mu in Guangliang alone, and there are many oil mills, with an annual output of nearly 45,000 tons of oil meal. According to reports, the oily meal contains 10% to 13% saponin, and based on this calculation, only Guangxi can produce about 4,500 tons of camellia oleifera saponin per year. Yet up to now, the research to camellia oleifera both at home and abroad is mainly camellia oil. The re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61K36/82
Inventor 王永奇冯宝民王威唐玲
Owner DALIAN UNIV
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