Method for efficiently extracting polysaccharide active ingredients in lycium barbarum by using ultrasound enhanced subcritical water

Abstract

The invention provides a method for efficiently extracting lycium barbarum polysaccharide. The method comprises the steps: adding lycium barbarum into an extraction kettle, injecting preheated deionized water to the extraction kettle at the flow speed of about 70mL / min by using a pump, controlling the liquid to solid ratio to be 10:1 to 30:1mL / g, simultaneously extracting the lycium barbarum polysaccharide at an extraction phase by using synergistic ultrasonic and subcritical water methods after heating to the preset temperature (larger than or equal to 100 DEG C), controlling the extraction pressure to 3-7MPa, the extraction temperature to 100-130 DEG C and the extraction time within 20-80min, and discharging an extracting solution after the extraction is ended, wherein the lycium barbarum is dried to the constant weight, the frequency of the ultrasonic is 18.5Hz, and the ultrasonic power is 100-140W; and after the extracting solution is filtered and subjected to vacuum concentration, settling for 12h by using 95% ethanol, removing a supernatant liquid and drying in vacuum to obtain the lycium barbarum polysaccharide. The yield of the lycium barbarum polysaccharide extracted by using the method is higher than the yields of lycium barbarum polysaccharides extracted by using a hot water extraction method, an ultrasonic extraction method and a subcritical water extraction method, and the antioxidant activity of the lycium barbarum polysaccharide extracted by using the method is stronger than the antioxidant activities of the lycium barbarum polysaccharides obtained by using the hot water extraction method, the ultrasonic extraction method and the subcritical water extraction method. Therefore, the method can lay the foundation for the industrial production and pharmacological research of the lycium barbarum polysaccharide.

Description

technical field [0001] The application of the present invention relates to the field of extraction of plant polysaccharides, in particular to the extraction process of Lycium barbarum polysaccharides in Chinese herbal medicine Lycium barbarum. Background technique [0002] Lycium barbarum is a multi-branched shrub of the genus Lycium barbarum in the family Solanaceae. Its fruit, Lycium barbarum, is sweet in taste and flat in nature. Modern research shows that wolfberry has broad application prospects in improving the body's immunity, invigorating qi and strengthening essence, anti-aging, anti-tumor, lowering blood fat and preventing atherosclerosis. Among them, Lycium barbarum polysaccharide is one of the main active components of Lycium barbarum, and a large number of studies have shown that it has obvious effects of anti-aging, anti-oxidation and enhancing immunity. [0003] The common extraction methods of Lycium barbarum polysaccharides are: hot water extraction, ultras...

AI Technical Summary

Problems solved by technology

Both of these two physical extraction methods can effectively improve the extraction yield of polysaccharides, and consume less time, but the extraction power used is generally large, and the number of extractions is > 1, which is likely to cause high energy consumption and easily destroy the molecular structure of polysaccharides. defect

The enzymatic method has strong selectivity, mild reaction conditions, and high extraction yield, but the price of the enzyme is relatively high, and the production cost is relatively high, so it is not suitable for industrial production

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