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Extraction method of high cell apoptosis induction active lycium barbarum polysaccharide

A Lycium barbarum polysaccharide and apoptosis-inducing technology, which can be used in drug combinations, anti-tumor drugs, etc., can solve the problems of complicated column chromatography operations, less attention to a single component, unfavorable for industry or production, and achieve good apoptosis induction. The effect of activity, low cost and short production cycle

Active Publication Date: 2013-02-27
天津肽谷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the existing technology mainly uses ultrafiltration technology to remove impurities, concentrate the extract, or use a method combined with chromatographic separation technology to separate and extract Lycium barbarum polysaccharides. The operation of column chromatography is complicated, which is not conducive to industry or production.
Moreover, although there are currently public literatures describing the extraction, separation and purification methods of Lycium barbarum polysaccharides, less attention has been paid to the purposeful acquisition of single components.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for extracting Lycium barbarum polysaccharide with high apoptosis-inducing activity, the specific steps are as follows:

[0039] (1) Extraction of Lycium barbarum polysaccharides: Quantitatively weigh 200g of dried Lycium barbarum, pulverize with a beater, add ethanol with a mass fraction of 70% in a ratio of 1:3 (g:ml), extract in a water bath at 70°C for 0.5h, and filter to obtain For the filter residue, add 70% ethanol at a ratio of 1:3 (g:ml), repeat the extraction twice under the condition of heating in a water bath, collect the filter residue, add distilled water to the filter residue at a ratio of 1:3 (g:ml), soak in a boiling water bath Extract for 1.5 hours, filter, collect the filtrate, repeat the extraction of the filter residue 3 times, combine the filtrate, and concentrate in vacuo to make the soluble solids greater than 10%; add absolute ethanol to a final concentration of 80% (v / v), filter and separate, Collect the precipitate, volatilize to remo...

Embodiment 2

[0047] A method for extracting Lycium barbarum polysaccharide with high apoptosis-inducing activity, the specific steps are as follows:

[0048] (1) Extraction of Lycium barbarum polysaccharides: Quantitatively weigh 200g of dried Lycium barbarum, pulverize with a beater, add ethanol with a mass fraction of 70% in a ratio of 1:3~6 (g:ml), extract in a water bath at 70°C for 0.5h, and filter , to get the filter residue, then add 70% ethanol at a ratio of 1:3~6 (g:ml), repeat the extraction 3 times under the condition of heating in a water bath, collect the filter residue, and add it to the filter residue at a ratio of 1:3~6 (g:ml) Add distilled water, extract in a boiling water bath for 1.5h, filter, collect the filtrate, repeat the extraction of the filter residue 3 times, combine the filtrate, and concentrate in vacuo to make the soluble solids greater than 10%; add absolute ethanol to a final concentration of 80% (v / v), separated (centrifuged at 3000r / min for 15min), collec...

Embodiment 3

[0056] A method for extracting Lycium barbarum polysaccharide with high apoptosis-inducing activity, the specific steps are as follows:

[0057] (1) Extraction of Lycium barbarum polysaccharides: Quantitatively weigh 200g of dried Lycium barbarum, pulverize with a beater, add ethanol with a mass fraction of 70% in a ratio of 1:3~6 (g:ml), extract in a water bath at 70°C for 0.5h, and filter , to get the filter residue, then add 70% ethanol at a ratio of 1:3~6 (g:ml), repeat the extraction 4 times under the condition of heating in a water bath, collect the filter residue, and add it to the filter residue at a ratio of 1:3~6 (g:ml) Add distilled water, extract in a water bath at 100°C for 1.5h, filter, collect the filtrate, repeat the extraction of the filter residue 3 times, combine the filtrate, and concentrate in vacuo to make the soluble solids greater than 10%; add absolute ethanol to a final concentration of 80% (v / v), separation (centrifugation at 3000r / min for 15min, or...

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Abstract

The invention relates to an extraction method of high cell apoptosis induction active lycium barbarum polysaccharide. The extraction method comprises using the fruit of Chinese wolfberry to serve as a raw material, obtaining lycium barbarum coarse polysaccharide through extraction, adopting an ultrafiltration membrane with 30K of molecular weight cutoff to separate the lycium barbarum polysaccharide, collecting percolate, using an ultrafiltration membrane with 10K of molecular weight cutoff to separate, collecting cutoff liquid 1, using an ultrafiltration membrane with 40K of molecular weight cutoff to separate, collecting percolate 1, conducting vacuum concentration and freeze drying on the cutoff liquid 1 and the percolate 1, and obtaining the high cell apoptosis induction active lycium barbarum polysaccharide. The extraction method uses a water extraction method to extract the lycium barbarum polysaccharide, removes protein, then adopts an ultrafiltration technique to grade and separate the polysaccharide, adopts the ultrafiltration membrane with a plurality of molecular weight cutoffs to repeatedly separate, overcomes shortcomings of being complex in column chromatography operation and unfavorable for industry or production and the like, is high in efficiency, low in cost, simple in operation, soft in condition, short in production phase and the like and provides a new thought for development of an active compound and industrialized application.

Description

technical field [0001] The invention belongs to the technical field of extracting active compounds, in particular to a method for extracting Lycium barbarum polysaccharide with high apoptosis-inducing activity. Background technique [0002] Lycium Barbarum polysaccharide (LBP) is a water-soluble polysaccharide extracted from Lycium barbarum. It is the main functional active ingredient of Lycium barbarum. The total content accounts for about 3.36% of the weight of Lycium barbarum. Lycium barbarum polysaccharide has a molecular weight range of more than 20,000, is soluble in water and hardly soluble in alcohol, and is mainly composed of six monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose, and is an acidic complex. polysaccharides. A large number of pharmacological studies and clinical medicine have shown that Lycium barbarum polysaccharide has various physiological functions such as lowering blood sugar, lowering blood fat, anti-inflammation, anti...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
Inventor 张民唐秀丽
Owner 天津肽谷生物科技有限公司
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