The invention relates to an extraction method of high cell apoptosis induction active lycium barbarum polysaccharide. The extraction method comprises using the fruit of Chinese wolfberry to serve as a raw material, obtaining lycium barbarum coarse polysaccharide through extraction, adopting an ultrafiltration membrane with 30K of molecular weight cutoff to separate the lycium barbarum polysaccharide, collecting percolate, using an ultrafiltration membrane with 10K of molecular weight cutoff to separate, collecting cutoff liquid 1, using an ultrafiltration membrane with 40K of molecular weight cutoff to separate, collecting percolate 1, conducting vacuum concentration and freeze drying on the cutoff liquid 1 and the percolate 1, and obtaining the high cell apoptosis induction active lycium barbarum polysaccharide. The extraction method uses a water extraction method to extract the lycium barbarum polysaccharide, removes protein, then adopts an ultrafiltration technique to grade and separate the polysaccharide, adopts the ultrafiltration membrane with a plurality of molecular weight cutoffs to repeatedly separate, overcomes shortcomings of being complex in column chromatography operation and unfavorable for industry or production and the like, is high in efficiency, low in cost, simple in operation, soft in condition, short in production phase and the like and provides a new thought for development of an active compound and industrialized application.