Extraction method of lycium barbarum polysaccharides

An extraction method, technology of Lycium barbarum polysaccharides, applied in the field of extraction of Lycium barbarum polysaccharides, achieves the effect of high yield and purity, and mild action conditions

Active Publication Date: 2015-01-28
百岳特生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The extraction rate of Lycium barbarum polysaccharide by the above-mentioned process is not high, so it is necessary to improve the above-mentioned method and design a high-yield extraction method of Lycium barbarum polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for extracting Lycium barbarum polysaccharides, the method comprising the steps of:

[0038] Select sun-dried wolfberry, crush it and pass through a 60-mesh sieve to obtain wolfberry powder, add water to the wolfberry powder and stir evenly, the weight ratio of wolfberry powder to water is 1:7.5, soak in water for 10 hours, add to the above solution Activated carbon, the activated carbon accounts for 2-6% of the weight of the wolfberry crude polysaccharide concentrate, stirs for 0.3h, and filters out the activated carbon; add cellulase, the addition of cellulase is 0.75%, the enzymolysis temperature is 45°C, and the pH value 5, the above-mentioned raw materials are placed in a microwave extraction equipment for extraction, the power of the microwave is 500W, the frequency is 800MHZ, and the enzymolysis is 0.3h;

[0039]Then add alkaline protease and ficin, the addition of alkaline protease is 0.45%, the addition of ficin is 0.4%, the enzymolysis temperature is ...

Embodiment 2

[0047] A method for extracting Lycium barbarum polysaccharides, the method comprising the steps of:

[0048] Select sun-dried wolfberry, crush it and pass through a 40-mesh sieve to obtain wolfberry powder, add water to the wolfberry powder and stir evenly, the weight ratio of wolfberry powder to water is 1:5, soak in water for 8 hours, add cellulase, fiber The added amount of suzyme is 0.5%, the enzymolysis temperature is 45°C, and the pH value is 5. The above-mentioned raw materials are extracted in a microwave extraction equipment. The power of the microwave is 400W, the frequency is 800MHZ, and the enzymolysis is 0.1h;

[0049] Then add alkaline protease and ficin, the addition of alkaline protease is 0.2%, the addition of ficin is 0.2%, the enzymolysis temperature is 50 ° C, the pH value is 6.8, and the above-mentioned raw materials are placed in microwave extraction equipment for extraction. The power of the microwave is 400W, the frequency is 800MHZ, the enzymolysis is ...

Embodiment 3

[0057] A method for extracting Lycium barbarum polysaccharides, the method comprising the steps of:

[0058] Select sun-dried wolfberry, crush it and pass through a 80-mesh sieve to obtain wolfberry powder, add water to the wolfberry powder and stir evenly, the weight ratio of wolfberry powder to water is 1:10, soak in water for 16 hours, add cellulase, fiber The added amount of suzyme is 0.9%, the enzymolysis temperature is 45°C, and the pH value is 5. The above-mentioned raw materials are extracted in a microwave extraction equipment. The power of the microwave is 600W, the frequency is 800MHZ, and the enzymolysis is 0.5h;

[0059] Then add alkaline protease and ficin, the addition of alkaline protease is 0.6%, the addition of ficin is 0.5%, the enzymolysis temperature is 50 ° C, and the pH value is 7.5, the above-mentioned raw materials are placed in microwave extraction equipment to extract, The power of the microwave is 500W, the frequency is 800MHZ, the enzymolysis is 0....

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PUM

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Abstract

The invention belongs to the technical field of polysaccharide extraction, and particularly relates to an extraction method of lycium barbarum polysaccharides. The extraction method comprises the following steps: selecting dried lycium barbarum, sieving at 40-80 meshes after smashing to obtain lycium barbarum powder, adding water to the lycium barbarum powder and stirring uniformly, soaking in water for 8-16 hours, and adding active carbon for decoloration, wherein the weight proportion of the lycium barbarum powder to water is 1:(5-10); adding cellulose for enzymolysis; then adding alkaline protease and ficus proteinase; carrying out enzymolysis under a microwave condition; carrying out concentration and decoloration after enzymolysis; centrifuging; taking supernate for concentration and alcohol precipitation, then centrifuging, washing and precipitating by adopting ethanol, smashing after freeze drying, and thus obtaining the lycium barbarum polysaccharides. The polysaccharides are extracted from the lycium barbarum by adopting the method disclosed by the invention, The action conditions of adopted enzymes are mild, fats and celluloses in the lycium barbarum are subjected to enzymolysis by using various different enzymes, and the polysaccharides in the lycium barbarum are extracted, so that the utilization rate of the lycium barbarum is improved; the polysaccharides obtained by using the method disclosed by the invention are not only high in yield but also high in purity.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to a method for extracting wolfberry polysaccharide. Background technique [0002] Lycium barbarum polysaccharide is a water-soluble polysaccharide extracted from Lycium barbarum. It has been clarified that the polysaccharide is a proteoglycan, which is composed of six monosaccharide components: arabinose, glucose, galactose, mannose, xylose, and rhamnose. Studies have shown that Lycium barbarum polysaccharides are the main active ingredients of Lycium barbarum to regulate immunity and delay aging. [0003] Most of the existing wolfberry polysaccharide extraction processes are traditional hot water extraction or hot water extraction after degreasing with organic solvents (ether, ethanol, petroleum ether, etc.). These conventional methods use a large amount of solvents, take a long time and have low extraction rates. In order to improve the economic b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 迟宗磊藏伟功丁振洋
Owner 百岳特生物技术(上海)有限公司
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