Method for inducing and identifying corn to generate haploid

A technology of haploid and haploid induction system, which is applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of reducing workload, wasting manpower and material resources, and haploid purity is not 100% , to achieve the effect of improving the purity

Active Publication Date: 2021-01-15
金苑(北京)农业技术研究院有限公司
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the Rl-nj gene is affected by the genetic background of the parents and the environment, and the color expression is strong or weak. Therefore, whether it is machine recognition or manual selection, due to the degree of color development of different genetic backgrounds, the threshold setting of the machine or the selection of personnel Proficiency is limited, the purity of selected haploids is not 100%, and there are a large number of hybrid plants in the field, which need to be continuously removed, which wastes a lot of manpower and material resources. If false haploids can be identified at an early stage, the follow-up workload will be greatly reduced

Method used

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  • Method for inducing and identifying corn to generate haploid
  • Method for inducing and identifying corn to generate haploid
  • Method for inducing and identifying corn to generate haploid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the preparation of maize haploid induction line

[0043] A maize haploid inducible line hybrid with red-root purple plant traits——Jinyuan Gaoyueza No. 1 was prepared according to the following method:

[0044] 1. Hybridization

[0045]In May 2015, 10 Nongda Gaoyu No. 3 plants were used as female parents, and 5 Nongda Gaoyu No. 1 plants were used as male parents for crossing, and 5 panicles were obtained from the F1 generation of Nongda Gaoyu No. 3, which were mixed for threshing;

[0046] In May 2015, 10 plants of Nongda Gaoyu No. 5 were used as female parents, and 5 Nongda Gaoyu No. 1 plants were used as male parents for crossing, and 5 ears of F1 generation derived from Nongda Gaoyu No. 5 were obtained, which were mixed and threshed.

[0047] 2. Backcross

[0048] In November 2015, 25 plants of the F1 generation from Nongda Gaoyu 3 and 25 plants of the F1 generation from Nongda Gaoyu 5 were used as female parents, and these F1 plants all showed red roo...

Embodiment 2

[0066] Example 2, the application of the maize haploid induction line Jinyuan Gaoyuza No. 1 in the induction of maize haploid

[0067] 1. Jinyuan Gaoyouza No. 1 induced maize haploid through artificial pollination

[0068] 1. Artificial pollination

[0069] The corn haploid induction method provided by the present embodiment consists of the following steps:

[0070] 1) At the end of April in spring, at the Jinyuan Seed Industry Breeding Base in Shunyi District, Beijing, the maize haploid induction line hybrid "Jinyuan Gaoyuza No. 1" (male parent) obtained in Example 1 was staggered for many times Ke 702 (Henan Jinyuan Seed Industry Co., Ltd.) was used as the induced material (female parent) in the second sowing period of the induced line. The female parent was sown with 110 plants, and the planting density was 4000 plants / mu.

[0071] 2) Female parent emasculates with bract leaves before tasseling ( figure 2 ), ear bagging. When the filaments come out, the pollen of the i...

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Abstract

The invention discloses a method for inducing and identifying corn to generate haploid. The invention provides a method for inducing corn to generate haploid. The method comprises the following stepsof A) preparing a corn haploid induction line hybrid with red-root purple plant characters; B) pollinating female parent corn by taking the corn haploid inducible line hybrid obtained in the step A) as a male parent, harvesting clusters, and sowing seeds with colorless embryos to obtain seedlings; and selecting seedlings with white root systems as haploid plants. According to the induction line 'Jinyuan high hybrid induction No.1' prepared by the method, the purity of the identified corn haploid can be remarkably improved by adopting a corn haploid induction identification technology, and thepurity is as high as 100% and is far higher than that of a control group (generally about 70%). According to the method, the key technical problem that the false haploid rate is too high in the haploid identification process is solved, and therefore the haploid identification efficiency is greatly improved.

Description

technical field [0001] The invention relates to the field of agricultural plant breeding, in particular to a method for inducing and identifying haploids in maize. Background technique [0002] Corn is already the food crop with the largest planting area and the highest total yield in my country. Almost all the corn varieties used in production are hybrids formed by inbred lines. The traditional pedigree method of breeding inbred lines adopts the method of continuous selfing, and it takes at least 8 generations from the composition to stability, and through the method of inducing haploid re-doubling, two generations are highly homozygous and stable, which is called double single The multiple breeding technology is very suitable for commercial breeding because it has the advantages of fast inbred lines, high homozygosity, accurate test results, and simple project management. [0003] At present, most of the maize haploid induction lines come from Stock6, which has two marker...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/02
CPCA01H1/02A01H1/04A01H1/08
Inventor 范弘伟叶凯侯丽宏康广华
Owner 金苑(北京)农业技术研究院有限公司
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