Method for raising induction rate of corn haploid inducing breeding

A technology of haploid induction and haploid induction line, applied in the directions of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of high cost and low induction rate, achieve good effect and improve breeding efficiency , the effect of low technology investment

Active Publication Date: 2016-07-20
HUBEI KANGNONG SEED IND LIMITED
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Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a method for increasing the induction rate of maize haploid

Method used

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Embodiment Construction

[0020] The technical solution in the present invention will be further described below in conjunction with the embodiments.

[0021] The corn haploid induction line involved in the embodiments of the present invention is as follows:

[0022] STOCK6: Purple-topped grains, high induction rate; recorded in "Xu Yanxia. Application of Stock6 in the Breeding of Maize Inbred Lines. "Heilongjiang Agricultural Sciences" 2014 No. 11"; the public can obtain it from Hubei Kangnong Seed Industry Co., Ltd available everywhere.

[0023] Lure FL18: Obvious genetic markers, large pollen amount, good disease resistance; no records, self-crossed 6 generations of STOCK6 mutant strains; the public can get it from Hubei Kangnong Seed Industry Co., Ltd.

[0024] A method for improving the induction rate of maize haploid induction breeding, comprising the steps of:

[0025] The corn haploid inducer line A was crossed with the corn haploid inducer line B, and the obtained F1 was the inducer hybrid C...

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Abstract

The invention provides a method for raising the induction rate of corn haploid inducing breeding.The method comprises the steps of conducting cross breeding on a corn haploid inducing line A and a corn haploid inducing line B, so that F1 serving as an inducing line cross-breeding seed C is obtained; taking C as a male parent, and taking a group D used for selecting a new material as a female parent, conducting cross breeding, and harvesting cross breeding current generation grains, so that corn haploid is obtained, wherein cross breeding of C and D comprises the following steps of watering a female parent plant with tap water with the PH value ranging from 5.5 to 8.0; conducting mist spraying treatment on filaments of the female parent plant which is watered with tap water with the same PH value with tap water with the PH value ranging from 5.5 to 8.0; after the filaments of the female parent emerge, conducting pollination cross breeding on the female parent with pollen of the male parent on the fifth day and the seventh day.By means of the method, the haploid inducing rate is effectively raised, breeding efficiency is improved, less investment is input, and the method is simple, convenient and easy to implement; meanwhile, agronomic traits of cross breeding of the inducing lines are greatly improved, and guarantee is provided for efficient and large-scale haploid induction.

Description

technical field [0001] The invention relates to the field of maize haploid induction breeding, in particular to a method for improving the induction rate of maize haploid induction breeding. Background technique [0002] Maize is a monoecious, sexually reproducing crop. The main way to increase maize yield is to use maize heterosis. The focus of maize breeding work is to develop maize hybrids. Breeding maize inbred lines is the basis for cultivating maize hybrids; through crossing between inbred lines to prepare maize combinations and breed new maize hybrids. Therefore, the technology of breeding maize inbred lines is the core technology of maize breeding. The traditional breeding technology of maize inbred lines requires many years of multi-generation self-selection and combining ability measurement technology to breed inbred lines with high combining ability and stable traits; and then breed new maize hybrids through crossbreeding . Breeding a new corn hybrid usually ...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 彭绪冰彭绪伟李大伟彭绪朋覃远照
Owner HUBEI KANGNONG SEED IND LIMITED
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