Pollination method for increasing maize haploid induced seed setting rate

A technology of haploid induction and haploid induction system, which is applied in botany equipment and methods, horticulture, application, etc., can solve the problems of inaccurate calculation of mis-date time, low induced seed setting rate, insufficient pollination, etc., and achieves Effects of improving maize haploid seed setting rate and induction rate, long duration of loose pollination, and low pollination cost

Inactive Publication Date: 2017-02-15
ZHANGYE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a pollination method for increasing the seed-setting rate of maize haploid induction. Natural pollination and artificial auxiliary pollination are used to improve the seed-setting rate and induction rate, and overcome the inability of the prior art to accurately calculate the wrong time, resulting in a failure of the flowering period. , Insufficient pollination, low induced seed setting rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of pollination method that improves corn haploid induced seed setting rate, its steps are as follows:

[0035] The male parent is the maize haploid induction line CAUHOI;

[0036] The female parent is Zheng Dan 958;

[0037] The female parent and the male parent are sown at intervals of a row ratio of 4:1, and the plant spacing in each row is 0.25m;

[0038] In each row of sowing the male parent, the male parent is sown in two phases, and the distance between the two phases of sowing is 1m. The male parent of the first phase calculates the number of leaves of the male parent and the female parent, and calculates according to the average growth of a corn leaf every 3 days. According to The number of leaves of the male parent and the female parent calculates the sowing date of the male parent, and the second phase is sown 7 days after the first phase of sowing, so as to ensure that the male parent and the female parent flowering period meet;

[0039] In addition,...

Embodiment 2

[0046] A kind of pollination method that improves corn haploid induced seed setting rate, its steps are as follows:

[0047] The male parent is the maize haploid induction line UH400;

[0048] The female parent is Zheng Dan 958;

[0049] The female parent and the male parent are sown at intervals with a row ratio of 5:1, and the plant spacing in each row is 0.20m;

[0050] In each row of sowing the male parent, the male parent is sown in two phases, and the distance between the two phases of sowing is 1m. The male parent of the first phase calculates the number of leaves of the male parent and the female parent, and calculates according to the average growth of a corn leaf every 3 days. According to The number of leaves of the male parent and the female parent calculates the sowing date of the male parent, and the second phase is sown 10 days after the first phase of sowing, so as to ensure that the male parent and the female parent flowering period meet;

[0051] In additio...

Embodiment 3

[0059] A kind of pollination method that improves corn haploid induced seed setting rate, its steps are as follows:

[0060] The male parent is the maize haploid induction line CAU5;

[0061] The female parent is Zheng 58 / PH6WC / / PH6WC;

[0062] The female parent and the male parent are sown at intervals of a row ratio of 4:1, and the plant spacing in each row is 0.25m;

[0063] In each row of sowing the male parent, the male parent is sown in two phases, and the distance between the two phases of sowing is 1m. Because the female parent's leaf number is unknown, it can only be roughly based on empirical female parent's leaf number=(Zheng 58 leaf number+PH6WC leaf number ) / 2 estimate, and then calculate according to the average growth of a corn leaf every 3 days, calculate the sowing date of the first phase of the male parent according to the number of leaves of the male parent and the female parent, sow the male parent, and sow the second phase 8 days after the first phase of ...

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Abstract

The invention relates to the maize breeding field and especially relates to a pollination method for increasing maize haploid induced seed setting rate. Inducing lines or hybrid strains thereof are used as male parents, and maize material expected to obtain haploid is used as female parents; the male parents and the female parents are sowed with intervals and the male parents are sowed by stages; besides, the male parents are sowed by stages in the central region of the sowing area for artificial auxiliary segment pollination. The segment auxiliary pollination method for increasing maize haploid induced seed setting rate prevents pollination but no seed setting due to the sterility of male parents and the female parents; the adopted hybrid pollinating method is combination of natural pollination and artificial segment auxiliary pollination so that full pollination of female parents is guaranteed and the seed setting rate and induction rate are increased.

Description

technical field [0001] The invention relates to the field of corn breeding, in particular to a pollination method for improving the haploid induced seed setting rate of corn. Background technique [0002] As a high-tech, haploid technology brings broad prospects for breeders to quickly select inbred lines. When inducing maize haploid, the inducing line and the base material are sown in a certain proportion at staggered intervals, isolated around the four sides, and the female parent material is artificially detasseled before pollination, so as to rely on the wind to spread the pollen of the male parent to achieve the purpose of pollination. The commonly used method of mis-stage calculation is to calculate according to the average growth of one corn leaf every 3 days. According to the number of leaves of the male parent and the female parent, sow at different stages. However, in haploid induction, most of the basic materials are newly assembled materials. , the number of lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01H1/02
CPCA01H1/02
Inventor 赵向田王长魁王托和郝铠陈晓军黄有成贾改秀
Owner ZHANGYE ACAD OF AGRI SCI
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