Culture medium for tissue culture of dragon fruits

A technology of tissue culture and culture medium, applied in the field of plant cultivation in horticulture, can solve the problems of long production cycle and achieve the effects of simplifying the cultivation procedure, shortening the production time and reducing the production cost

Active Publication Date: 2017-06-20
钦州市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention is to overcome the deficiencies of the existing formula, to provide a kind of culture medium of pitaya tissue culture, to solve the problem of long production cycle occurring in the cultivation process of pitaya tissue culture seedlings

Method used

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  • Culture medium for tissue culture of dragon fruits
  • Culture medium for tissue culture of dragon fruits
  • Culture medium for tissue culture of dragon fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of substratum of pitaya tissue culture, adopt this substratum to cultivate the kind to be the pitaya of H-5, implement season is summer and autumn, use formula as follows:

[0031] (1) Inorganic nutrients:

[0032] ①A large number of elements

[0033]

[0034] ②Trace elements

[0035]

[0036] (2) Organic nutrients

[0037]

[0038] (3) Plant growth regulator substances

[0039] Cytokinin 6-benzyl adenine (6BA) 0.55mg / L

[0040] Auxin naphthaleneacetic acid (NAA) 0.15mg / L

[0041] (4) Other additives

[0042] Activated carbon (Ac) 100mg / L

[0043] (5) Sugar 35g / L

[0044] (6) Agar (hardness 1200) 3.0g / L

[0045] The balance is distilled water.

[0046] The results of the cultivation are: the multiplication coefficient is 6.5, the rooting rate is 87.65%, the production cycle is 40 days, and the production cost is 0.60 yuan / plant.

Embodiment 2

[0048] A kind of substratum of pitaya tissue culture, adopting this substratum to cultivate the kind is the pitaya of H-5, and implementing season is winter-spring, and using formula is as follows:

[0049] (1) Inorganic nutrients:

[0050] ①A large number of elements

[0051]

[0052] ②Trace elements

[0053]

[0054]

[0055] (2) Organic nutrients

[0056]

[0057] (3) Plant growth regulator substances

[0058] Cytokinin 6-benzyl adenine (6BA) 0.55mg / L

[0059] Auxin naphthaleneacetic acid (NAA) 0.15mg / L

[0060] (4) Other additives

[0061] Activated carbon (Ac) 100mg / L

[0062] (5) Sugar 35g / L

[0063] (6) Agar (hardness 1200) 3.0g / L

[0064] The balance is distilled water.

[0065] The results of the cultivation are as follows: the multiplication coefficient is 6.0, the rooting rate is 85%, the production cycle is 50 days, and the production cost is 0.65 yuan / plant.

[0066] Comparison with existing technology:

[0067] Table 1 adopts the main param...

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Abstract

The invention discloses a culture medium for tissue culture of dragon fruits. The culture medium not only can be used for culturing subculture plantlets, but also can be used for inducing rooting seedlings, i.e., culture steps are simplified, subculture enrichment culture and rooting induction culture are completed by one step, the production period is greatly shortened, and cost is saved. The invention provides the efficient culture medium for the tissue culture of the dragon fruits so as to solve the problems of long production period and high production cost in the dragon fruit cultivating process.

Description

technical field [0001] The invention relates to plant cultivation techniques in horticulture, in particular to a culture medium for dragon fruit tissue culture. Background technique [0002] Dragon fruit is a trade name, and its scientific name is Hylocereus undatus (Haw.) Britt. Et Rose), which is a plant of the genus Hylocereus of the cactus family (Cactaceae). [0003] Pitaya is named for its fleshy scales resembling the outer scales of a dragon. It is also called red dragon fruit, dragon pearl fruit, fairy honey fruit, and jade dragon fruit. It is a tropical and subtropical fruit and a perennial climbing succulent. Pitahaya flowers are clean and huge, full of fragrance when blooming, bright in appearance and sweet in taste. It is a kind of healthy and high-quality tropical fruit emerging in my country in recent years, with high economic value. At present, there are large-scale plantings in Guangdong, Guangxi, Fujian, Hainan, Yunnan, Guizhou and other provinces, and a sm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 李清香陈丽文吴红英杨利平时群吕月保陶建华张桂兰何克钦黄永钦陈乃明
Owner 钦州市林业科学研究所
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