Creation method of paddy rice common nuclear sterile line and application

Abstract

The invention discloses a creation method of a paddy rice common nuclear sterile line and application. The creation method is characterized a common fertile breeding material is used to create the sterile line, the sterile line is hybridized with an intermediate male parent material, hybrid seeds are sorted according to sorting gene expression characters to obtain a large number of sterile-line seeds which do not contain transgenic components, and the reproduction of the common nuclear sterile line is achieved. The common nuclear sterile line and a restoring line are used to produce the hybridseeds, the breeding procedure is simple, a large amount of backcross transformation time can be saved, the sterile characters of the common nuclear sterile line are fast imported into the fertile material, and the breeding cycle is shortened greatly.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, and in particular relates to a creation method and application of a common nuclear male sterile line of rice. Background technique [0002] Rice is an important food crop in the world. About 50% of the world's population uses rice as a staple food. Rice is also a raw material for processing many foods. The "three-line method" and "two-line method" are the main ways to utilize rice hybrid vigor at present, but the problem with the "three-line method" is that it is limited by the strict restoration relationship, and the parental resources that can be used are limited; the "two-line method" There is a greater risk in the safety of seed production, which limits the potential of these two approaches to fully exploit rice heterosis. [0003] Common GMS has the characteristics of a wide range of sources, no restriction of restoration relationship, complete abortion and no environmental influence ...

AI Technical Summary

Problems solved by technology

The problem encountered in the application is: the common GMS resources required for the construction of the "third-generation hybrid rice" system require the common GMS genes that have been genetically cloned (that is, genes with relatively thorough basic research) at the genome level Changing the expression of genes at a fixed point, thereby changing the target traits. The materials derived from these mutants are usually not the materials that breeders are most looking forward to using directly (materials for basic research are not the best and latest materials in production), so these materials should be For production, it must be transferred through backcrossing, etc., which is a time-consuming and heavy workload.

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