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Selective breeding method for rape cytoplasmic male sterile line

A male sterile line and male sterility technology, applied in the directions of botanical equipment and methods, application, and plant genetic improvement, can solve problems such as affecting the breeding effect, and achieve stable and thorough sterility, simple breeding procedures, and breeding purposes. clear effect

Inactive Publication Date: 2007-05-23
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main disadvantage of Pol cms is that the fertility of the sterile line is affected by the degree of temperature due to the difference in the nuclear gene background, that is, fertile pollen appears under low or high temperature conditions, which affects the breeding effect

Method used

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  • Selective breeding method for rape cytoplasmic male sterile line
  • Selective breeding method for rape cytoplasmic male sterile line
  • Selective breeding method for rape cytoplasmic male sterile line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The cultivation of embodiment 1 mustard type rape cytoplasmic male sterile line

[0029] 1. The steps of genetic classification and molecular marker-assisted breeding of cytoplasmic male sterile lines of Brassica napus are as follows:

[0030] 1. Determination of recovery relationship:

[0031] (1) The mustard-type cytoplasmic male sterile lines were genetically classified through the determination of the restoration relationship, and 6-102A was identified as a new sterile cytoplasmic type.

[0032] (2) Through the determination of the recovery relationship (see literature: Fu Tingdong, "Breeding and Utilization of Hybrid Rapeseed", Hubei Science and Technology Press, 2000, select 39 Brassica napus and Brassica napus varieties or lines respectively with pol CMS; ogu CMS, tour CMS, nap CMS, and 6-102A rapeseed cytoplasmic male sterile materials were used for test crossing, and the results of the determination of the restoration relationship are shown in Table 2:

[003...

Embodiment 2

[0053] Example 2 Obtaining the Cytoplasmic Male Sterile Line of Brassica napus by Backcross Breeding

[0054] According to the breeding steps described in "two" in Example 1, the cytoplasmic male sterile line 6-102A of Brassica napus was used as the female parent, and the Brassica napus variety Huashuang No. 3 (a conventional double-low rape variety publicly used) was used as the female parent. As the recurrent parent, the cytoplasmic male sterile line of Brassica napus was obtained through eight consecutive generations of backcross selection. The propagation procedure of the cytoplasmic male sterile line is the same as that of the mustard rape cytoplasmic male sterile line 6-102A.

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Abstract

The invention relates to a method for selecting male sterility line of rape cytoplasm, wherein the invention comprises that: selecting wide mustard sterility male, using mustard rape as hold line; crossing and molecule mark to assist the selection, to obtain the mustard rape cytoplasm male sterility line and hold line with stable generation; cultivating said sterility line to obtain usable mustard rape cytoplasm male sterility line.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to the breeding, propagation and utilization of a new rapeseed cytoplasmic male sterile line. Background technique [0002] Cytoplasmic male sterility is the most important pathway of heterosis in rapeseed. At present, there are many types of cytoplasmic male sterility reported at home and abroad. According to their different sources, they can be divided into two categories: heterologous cytoplasmic male sterility and homologous cytoplasmic male sterility: in heterologous CMS, the most useful and has begun There are ogu CMS, kosena CMS and Tour CMS for commercial breeding; among the homologous CMS, the one with commercial value is mainly Polima cytoplasmic male sterile line (Pol cms). Pol cms was discovered and invented by academician Fu Tingdong of Huazhong Agricultural University in 1972, the first practical rapeseed male...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00A01H1/02A01H1/04
Inventor 傅廷栋涂金星马朝芝万正杰李兴华
Owner HUAZHONG AGRI UNIV
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