Breeding and propagation method of brassica campestris

Abstract

The invention relates to a breeding and propagation method of brassica campestris. A cabbage cytoplasmic male sterile line CMS65-7 is taken as a donor of sterile cytoplast of the cytoplasmic male sterile line, a purple-leaf cabbage selfing line 08-131 and a purple-leaf mustard selfing line 08-550 are taken as donors with high anthocyanin character, brassica campestris 15-141 is taken as a donor with brassica campestris character, and a brassica campestris cytoplasmic male sterile line and a maintenance line which have stable heredity, high disease resistance, excellent growth characters and high anthocyanin content are obtained through distant hybridization, embryo rescue, interspecies cross and multi-generation continuous back cross in combination with cultivation trials, evaluation of disease resistance, evaluation of anthocyanin content and fertility evaluation. The strains have the characteristic of high anthocyanin content and have the fertility of class 0 and the sterility rate and sterility degree of 100%.

Description

Technical field [0001] The invention belongs to the technical field of selection and breeding of new vegetable varieties of Brassica of the cruciferous family, and relates to a method for the selection and breeding of Wutacai. Background technique [0002] Wu Ta Cai ( Brassica campestris L. ssp. chinensis var . rosularis Tsen , AA, 2n=20) is a leafy vegetable of the Brassica genus of the cruciferous family. At present, there are usually three ways to select varieties of Wutacai: 1. Conventional varieties; 2. The first generation of hybrids bred using cytoplasmic male sterile lines as the female parent. The sterile cytoplasms are mainly ogu CMS and pol CMS; 3. The first generation of hybrids bred by self-incompatible lines as the female parent. Among them, the first hybrids selected from conventional varieties and cytoplasmic male sterile lines accounted for the main part of Wutacai varieties. F selected by cytoplasmic male sterile line 1 Due to the excellent heterosis, it ha...

AI Technical Summary

Problems solved by technology

In the breeding of new varieties dominated by male sterility, the long-term single cytoplasmic type is likely to cause an increase in the incidence rate, thereby affecting crop yield; at the same time, ogu CMS cytoplasmic nectaries degenerate, resulting in low seed production, and pol CMS cytoplasmic Poor stability, fertility is easily affected by temperature, reducing hybrid purity

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