The invention discloses a cultivation method for temperate deciduous fruit trees in a subtropical region. The cultivation method comprises the steps that in September to October every year, 200 g of urea, 1,500 g of calcium superphosphate, 300 g of potassium fertilizer, 100 g of zinc sulfate and 100 g of borax are applied to each fruit tree, deep pits with the depth of 2,400-2,600 cm are dug on the sides of roots, and after the fertilizer is applied to the deep pits, the deep pits are covered with soil and then watered; 15-25 days before leaves fall, 0.3%-0.5% of urea and 0.2%-0.4% of potassium fertilizer are sprayed to the leaf surfaces of the fruit trees once; paclobutrazol is applied to soil according to the fruit tree projection area of 6.5-7.5 g/m<2>, and/or 100-200 times of PBO is sprayed to the fruit trees 2-3 times from August to October every year; diseased branches, pest branches, rotten fruits and leaves are removed and deeply buried or incinerated, and disinfection, sterilization and pest egg killing are conducted on the under-forest ground with quick lime according to the specification of 40-60 kg/mu, and the fruit tree ground is deep ploughed. The cultivation method has the advantages that cultivation operation and management are easy and convenient, little disease damage is generated, and fruits are high in yield and quality.