Novel method of using land and greenhouse for anniversary cultivation of fourth-generation winter wheat

Abstract

The invention discloses a novel method of using land and greenhouse for anniversary cultivation of fourth-generation winter wheat. Anniversary cultivation of fourth-generation winter wheat is realized by means of the techniques of embryo seedling, land and greenhouse combined on-site generation acceleration, and the like. The method includes: selecting 14-16d embryos to inoculate to an MB culture medium, so that the embryo seedling rate and embryo seedling transplanting survival rate are high; placing embryo seedlings in an illumination incubator for vernalization for 20-25d at the temperature of 0-6 DEG C; explicating the culture time, place and temperature and light conditions of one-year four-generation cultivation of winter wheat, wherein the greenhouse is 25-30 DEG C in winter, night light supplementation is 1000LX, and natural temperature and light are used in other time for generation accelerated cultivation; and transplanting the embryo seedlings to the land in the midmonth of June. The flowering period avoids the great heat period, and embryo seedling cultivation management measures are taken for increasing the embryo seedling transplanting survival rate and ripening rate. In addition, the method is simple, convenient, feasible, low in cost, high in efficiency and easy to popularize, and is a quick and efficient method provided for winter wheat seed breeding.

Description

(1) Technical field [0001] The invention relates to a new method for cultivating four-generation winter wheat by utilizing field and greenhouse anniversaries, belonging to the field of crop breeding. (2) Background technology [0002] Accelerating the breeding process of winter wheat and shortening the breeding cycle are important issues in the research of rapid breeding of winter wheat. Wang Hengli (1987), Guo Wenxiu (1993), Mi Guohua et al. (1998), Yao Jinbao et al. Variety characteristics, plant mature wheat seeds, and carry out wheat breeding. Ding Xiaoyi et al. (2005) adopted the in vitro culture technology of immature embryos to break seed dormancy, so that immature embryos did not produce callus, but directly produced seedlings. This shortens the breeding time. [0003] The deficiencies in the above methods are that the first is to use the special geographical environment and the climatic conditions in different regions to add generations, which has certain limitat...

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Problems solved by technology

[0003] The deficiencies in the above methods are that the first is to use the special geographical environment and the climatic conditions in different regions to add generations, which has certain limitations; the second is to use wheat varieties with strong spring properties, which is not conducive to popularization and application; the third is to use mature wheat seeds to add generations, Mature seeds need to be sown. Compared with using young embryos to form seedlings in one step, the time from pollination to immature embryos is longer; the fourth is to use the technology of in vitro culture of young embryos to transplant the embryo seedlings to the greenhouse for additional generations. Ventilation and cooling, high cost of scientific research

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