Sterile sowing method for ginseng seeds

A technology for ginseng seeds and aseptic sowing, which is applied in seed immunization, seed and rhizome treatment, germination equipment, etc., and can solve problems such as difficult germination and low germination rate

Inactive Publication Date: 2016-05-04
黄庆梅
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Problems solved by technology

[0003] However, when ginseng seeds mature, they will accumulate a large amount of substances that inhibit germination, prevent the germination of ginseng seeds, and make ginseng seeds enter deep dormancy. Conventional aseptic seeding methods have difficulty in germination and low germination rates.

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  • Sterile sowing method for ginseng seeds

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Embodiment Construction

[0015] The present invention will be further described below in conjunction with specific embodiments.

[0016] According to the 1 / 2MS medium formula shown in Table 1, weigh each component in 1 / 2MS medium and 1.0 mg of naphthaleneacetic acid, 1.5 mg of 6-benzylaminoadenine, 10 of agar powder and 30 g of Put white sugar in a conical flask, add an appropriate amount of purified water, stir to dissolve, set the volume to 1000mL, and adjust the pH value to 6.2. Seal the triangular flask, place it in an autoclave, and sterilize it at 121°C for 21 minutes, then put the culture medium into tissue culture bottles in an ultra-clean workbench, cool naturally, and seal the tissue culture bottles after they solidify ,spare.

[0017] Table 11 / 2 Composition and dosage of MS medium.

[0018]

[0019] Take the mature ginseng seeds with full grains, soak the ginseng seeds in cold water at 10°C for 20 hours, take out the seeds, and soak the seeds in 75% alcohol for 30 seconds in an ultra-c...

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Abstract

The invention provides a sterile sowing method for ginseng seeds, and belongs to the technical field of ginseng tissue culture. The method includes the steps of seed surface disinfection, inoculation pretreatment and inoculation, cold water immersion is used for simulating vernalization, ginseng seed dormancy is broken through ultrasonic treatment and KOH solution immersion treatment, and ginseng seeds are induced to germinate. The sterile sowing method for the ginseng seeds has the advantages of being low in cost and high in germination rate.

Description

technical field [0001] The invention belongs to the technical field of ginseng tissue culture, in particular to a method for aseptic sowing of ginseng seeds. Background technique [0002] Ginseng is an angiosperm, dicotyledonous plant class, subclass Araliaceae, a perennial herb plant, growing in dense forests, mostly distributed in the deep mountains of Heilongjiang, Jilin, Liaoning and northern Hebei, containing more than 10 kinds of ginsenosides, and ginseng fast Alcohol, a variety of amino acids and vitamins, is a valuable Chinese herbal medicine. [0003] However, when ginseng seeds mature, they will accumulate a large amount of substances that inhibit germination, prevent ginseng seeds from germination, and cause ginseng seeds to enter deep dormancy. Conventional aseptic sowing methods have difficulty in germination and low germination rates. Contents of the invention [0004] Based on the above-mentioned problems in the prior art, the present invention provides a m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/08A01C1/00A01C1/02
CPCA01C1/08A01C1/00A01C1/02
Inventor 何志铿
Owner 黄庆梅
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