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Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture

A technology of germination culture and sterile seedlings, applied in the field of plant tissue culture, can solve the problems of destroying the pokeweed seed coat structure, damaging the seed contents, and the hard pokeweed seed coat, etc., to achieve the effect of shortening the germination and seedling time

Inactive Publication Date: 2009-06-10
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aiming at the problem that pokeweed seed coat is too hard, the present invention uses concentrated sulfuric acid to soak pokeweed seeds for a certain period of time, so as to destroy the structure of pokeweed seed coat (but at the same time, the inclusions of the seeds cannot be damaged), and break the dormant state of the seeds, so that the seeds can quickly absorb and enter the germination state

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The pokeweed fruit and seeds were picked at the end of September when the pokeweed fruit and seeds were ripe, and the fully ripe and insect-free fruits were selected to dry in the sun. After the pokeweed fruit was completely dry, the pokeweed fruit was rubbed with hands to make the seeds fall off from the fruit. Sorting is then carried out, after which the seeds are left to dry in the sun until they are dry. Store the sun-dried seeds in a cool place in air-permeable bags.

[0020] Take mature pokeweed seeds, wash them in running water for 3 hours, and drain the water. Take 200 seeds from it and put them into a 100mL reagent bottle, pour 50mL of concentrated sulfuric acid, shake gently, and soak for 15min. Then pour out the concentrated sulfuric acid, rinse the seeds with water 8 times, and remove the residual concentrated sulfuric acid. Place the soaked and rinsed seeds on filter paper to drain and set aside.

[0021] Sow the above-treated pokeweed seeds in flowerpot...

Embodiment 2

[0023] Take mature pokeweed seeds, wash them in running water for 3 hours, and drain the water. Take 100 seeds from it and put them into a sterile 100mL reagent bottle, pour 30mL of concentrated sulfuric acid into it, shake gently, and soak for 15min. Then pour out the concentrated sulfuric acid, rinse the seeds 8 times with sterile distilled water to remove residual concentrated sulfuric acid. Place the soaked and rinsed seeds on sterile filter paper to drain and set aside.

[0024] Under sterile conditions, the pokeweed seeds of the above-mentioned treatment were inoculated in the 150mL culture bottle filled with 50mLMS solid medium (MS medium+2% sucrose+0.7% agar), 5 grains per bottle, and 6 bottles were inoculated; then Place them in the dark at 26±1°C, count the germination rate of the seeds after one week, and then transfer the culture bottle to a light incubator at 26±1°C with a light intensity of 2000LUX for cultivation, and two weeks later you can get pokeweed asepti...

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Abstract

The invention relates to the field of plant tissue culture, in particular to a method for quickly germinating phytolacca americana seeds and application of the method to aseptic seedling culture. The method comprises: soaking the phytolacca americana seeds into a concentrated sulfuric acid for a certain period, damaging the structure of phytolacca americana seed coats, breaking the sleep state of the seeds, and making the seeds be quickly subjected to imbibition and enter the germination state; simultaneously, utilizing the sterilization function of the concentrated sulfuric acid to perform sterilization treatment on the phytolacca americana seeds; subsequently, inoculating the aseptic phytolacca americana seeds on a culture medium selected by an experiment, adjusting the conditions such as temperature and illumination, and making the phytolacca americana seeds germinate quickly; and finally obtaining aseptic phytolacca americana seedlings. The method can quickly break through the sleep state of the phytolacca americana seeds, and greatly shorten the germination time and the seedling time of the phytolacca americana seeds. The invention provides a method for culturing the aseptic phytolacca americana seedlings for laboratory investigation.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for rapid germination of pokeweed seeds and the application of the method in sterile seedling cultivation. technical background [0002] Pokeweed is a perennial herb with many functions. At present, people mainly use pokeweed to pick wild resources. This is mainly because pokeweed is difficult to reproduce and is not conducive to large-scale planting. Pokeweed is generally propagated by seeds, but due to the hardness of the seed coat, compact structure, and long dormancy period, the germination of pokeweed seeds often lasts for one to several months, and the germination rate is not high, which makes the growth cycle of pokeweed It takes months or even years to get the metabolites people want. In view of the difficulty in germination of pokeweed, some people try to break the dormant state of pokeweed seeds and promote rapid germination by light treatment, variable tem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 郑铁松陈静
Owner NANJING NORMAL UNIVERSITY
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