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Efficient aseptic seedling raising method of monochasma sheareri

A deer antler grass and high-efficiency technology, applied in the field of plant tissue culture, can solve the problems of complex surface disinfection methods, unsatisfactory disinfection effect, uneven inoculation, etc., and achieve the effect of solving the problem of low natural germination rate, maintaining vitality, and growing neatly.

Inactive Publication Date: 2019-11-26
EXPERIMENTAL CENT OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] In order to solve the problems that conventional seed treatment and surface disinfection methods are relatively complicated, easily cause seed loss, unsatisfactory disinfection effect and uneven inoculation, the present invention proposes a sterile and high-efficiency seedling raising method of velvet antler, which can effectively obtain clean seeds and break seed dormancy , to ensure the disinfection effect of the seed surface, so that the seeds are evenly dispersed on the surface of the medium, and the operation is simple and easy, which can improve the utilization rate of the seeds, obtain a large number of velvet grass seedlings in a short time, and protect the velvet grass plant resources

Method used

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  • Efficient aseptic seedling raising method of monochasma sheareri

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Embodiment Construction

[0039] Below in conjunction with experimental table, the present invention will be further described:

[0040] Figure 1-3 Shown, a kind of velvet antler aseptic high-efficiency seedling raising method comprises the steps:

[0041] (1) Seed collection: From the end of April to the beginning of May, when the capsules turn from green to yellow or brown and the tips are slightly cracked, collect mature capsules of velvet grass in batches and place them in airtight plastic bags;

[0042] (2) Seed treatment: the capsules dried in the shade for 1-2 days are gently rubbed with hands to remove the peel, passed through a 0.25mm sieve, and the seeds are collected and placed in a sealed EP tube and stored in the refrigerator at 4°C for later use;

[0043] (3) Place the seeds stored in EP tubes at low temperature on wet filter paper or place them in humid air at room temperature for 12 hours to make them slowly imbibition. The newly collected velvet grass seeds can omit the imbibition st...

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Abstract

The invention discloses an efficient aseptic seedling raising method of monochasma sheareri. According to the efficient aseptic seedling raising method of the monochasma sheareri, through the steps ofseed treatment, surface sterilization, inoculation, transplanting and the like, the highest seed germination rate of the monochasma sheareri reaches 100%, and the transplanting survival rate reaches86%; by adopting the inoculation method, monochasma sheareri seeds are distributed in a culture medium more uniformly, cultivated aseptic seedlings are healthy and strong and grow in order, the cleanseeds can be effectively obtained, seed dormancy is broken, and the seed surface sterilization effect is ensured, so that the seeds are dispersed on the surface of the culture medium uniformly; the operation is simple and easy, the seed utilization rate can be increased, a large number of monochasma sheareri seedlings can be obtained in a short time, and wild monochasma sheareri plant resources can be effectively protected.

Description

technical field [0001] The invention specifically relates to a method for aseptic and high-efficiency seedling cultivation of velvet antler, belonging to the technical field of plant tissue culture. Background technique [0002] Deer antler is a perennial semi-parasitic small herb of the family Scrophulariaceae. It is mainly distributed in Jiangxi, Zhejiang, Jiangsu, Fujian and other places in southeastern my country. It grows in weeds on sunny hillsides or under Pinus massoniana forests. It is reported in the literature that velvet antler contains a variety of secondary metabolites, which have antibacterial, anti-inflammatory and antiviral effects. It is often used clinically to treat colds, coughs, pneumonia, toothache and other diseases. It is an important understory medicinal plant. [0003] Studies have found that luteolin, a flavonoid component with various biological activities such as anti-oxidation, antibacterial and anti-inflammatory, immune enhancement, anti-cance...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李峰卿曾满生刘素贞姚甲宝
Owner EXPERIMENTAL CENT OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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