Multiplex PCR detection kit and identification method for mouse meat, goat meat and mutton

Abstract

The invention relates to the technical field of food detection, in particular to a multiplex PCR detection kit for mouse meat, goat meat and mutton, which is characterized by comprising an animal tissue mitochondrial DNA extraction system and three multiplex PCR reaction systems (a positive control reaction system, a negative control reaction system and an identification reaction system). The total PCR reaction system is 50 microliters, containing 5 microliters of reaction buffer solution, 2-5 microliters of dNTP, 23 microliters of MgCl, 1-5 microliters of Taq DNA polymerase, 0.5-2 microlitersof each primer, 1-3 microliters of DNA of a sample to be detected, and the balance being double distilled water. A multiplex PCR identification method for the mouse meat, the goat meat and the muttoncomprises the steps of designing three specific oligonucleotide primers by utilizing species specificity of cytochrome b, determining a reaction procedure, judging results and the like; the multiplexPCR identification method can simultaneously distinguish the specificity of the mouse meat, the goat meat and the mutton; and the multiplex PCR identification method for the mouse meat, the goat meatand the mutton has the advantages of simplicity, convenience, rapidness, reliable detection result and the like.

Description

technical field [0001] The invention relates to the application of molecular biology technology in the detection field of meat products, more specifically, it is a rapid DNA identification kit for mouse meat (rat, mouse, guinea pig, Rattus norvegicus), goat meat and sheep meat and Detection method. Background technique [0002] Meat food is an edible product for people's livelihood, and its quality is related to the health and life safety of the people. In daily life, the mutton we eat is mostly sheep and goat. Goat meat is the meat of Qingyang, a bovid animal. Tonifying deficiency and supporting yang. Control consumptive internal injuries, weakness of muscles and bones, soreness and weakness of the waist and spine, impotence, leukorrhea, and infertility. Among the goat meat, there is a traditional high-quality mutton called Baizi mutton, which is famous for its tenderness, fragrance, and no fishy smell. It is known as "earth ginseng" and "buxin pill". Since the 1980s, ...

AI Technical Summary

Problems solved by technology

However, the instruments and reagents used are expensive, time-consuming, require high testing technicians, and difficult to operate, so it is not suitable for rapid batch testing.

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