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Mink heart DNA detection kit and identification method
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A detection kit and identification reaction technology, applied in biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the difficulties in the quality control of mink heart and its preparations, the difficult identification of mink heart in traditional Chinese medicine, and the inability to distinguish mink heart from mink heart Easy to mix with animal heart and other problems, to achieve the effect of simple identification method and reliable test results
Inactive Publication Date: 2011-03-23
BEIHUA UNIV
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At present, there is no legal identification method for mink heart in the national standard, and there are no literature reports on the composition research and identification of mink heart at home and abroad. The quality control of mink heart and its preparations has brought great difficulties, and it is difficult to meet the identification of the traditional Chinese medicine mink heart
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[0029] The present invention will be further described below using examples.
[0030] A detection kit for mink heart DNA, which comprises:
[0031] (a) Identification reaction system: the total system is 100 μL, which contains buffer 10 μL, 12.5 mMdNTP 4-10 μL, 0.1 mM primer 1 and primer 2 1.5-4.0 μL, Taq DNA polymerase 2-10 μL, to be tested Sample DNA 2-5μL, the rest is made up to 100μL with double distilled water;
[0032] (b) Positive control reaction system: the total system is 100 μL, which contains 10 μL buffer, 12.5 mM dNTP 4-10 μL, 0.1 mM primer 1 and primer 2 1.5-4.0 μL, Taq DNA polymerase 2-10 μL, Mink heart DNA 2-5μL, and make up to 100μL with double distilled water;
[0033] (c) Negative control reaction system: the total system is 100 μL, which contains 10 μL buffer, 12.5 mM dNTP 4-10 μL, 0.1 mM primer 1 and primer 2 1.5-4.0 μL, Taq DNA polymerase 2-10 μL, Chicken heart, duck heart, goose heart and rabbit heart DNA were mixed at a ratio of 1:1 to form a mixture...
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Abstract
The invention relates to the Chinese medicine detection technology field and belongs to a mink heart DNA detection kit, comprising buffer solution, 12.5mM dNTP, one type of 0.1mM primer 1 or 2, Taq DNA polymerase, sample DNA to be detected, and the identification reaction system of double-distilled water; containing buffer solution, 12.5mM dNTP, one type of 0.1mM primer 1 or 2, Taq DNA polymerase, mink heart DNA, and the positive control reaction system of the double-distilled water; including buffer solution, 12.5mM dNTP, one type of 0.1mM primer 1 or 2, Taq DNA polymerase, mixture of chicken heart, duck heart, goose heart and rabbit heart DNA according to 1:1, and the negative control reaction system of the double-distilled water. The mink heart DNA identification method comprises stepssuch as designing mink heart mitochondrial DNA two-pair specific oligonucleotide primer, artificially synthetizing mink heart mitochondrial DNA two-pair specific oligonucleotide primer, fixing reaction procedures and result judgment, and the like; the method can simultaneously and accurately differ the specificity of various animal hearts which are easily mixed with the mink heat; and the identification method has the advantages of simplicity, rapidness, and reliable detection result, etc.
Description
technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicines, in particular to a detection kit and identification method for mink heart DNA. Background technique [0002] Mink heart is a precious medicinal material, and it is the main drug in Lixin Pills, a Chinese patent medicine. Lixin Pill has the effect of invigorating the heart and calming the nerves, and can treat rheumatic heart disease, tachycardia, arrhythmia and heart failure. At present, there is no legal identification method for mink heart in the national standard, and there is no literature report on the composition research and identification of mink heart at home and abroad. The quality control of mink heart and its preparations has brought great difficulties, and it is difficult to satisfy the identification of mink heart, a traditional Chinese medicine. Contents of the invention [0003] The present inventor follows mitochondrial DNA (mtDNA) as the ca...
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