Method for analyzing flavonoid chemical components in walnut flowers

A chemical composition, technology of walnut flower, applied in the field of analysis of flavonoid chemical composition in walnut flower, to achieve the effect of efficient identification method

Inactive Publication Date: 2018-01-19
CHINESE ACAD OF INSPECTION & QUARANTINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the components of walnut flowers is mainly based on flavonoids, and there is no report on the analysis of flavonoids in walnut flowers using ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry

Method used

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  • Method for analyzing flavonoid chemical components in walnut flowers
  • Method for analyzing flavonoid chemical components in walnut flowers
  • Method for analyzing flavonoid chemical components in walnut flowers

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Experimental program
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Embodiment Construction

[0024] 1. Instruments and materials

[0025] The walnut flower sample was identified as Juglandaceae Juglans Juglans stylus by Professor Lei Haimin of Beijing University of Traditional Chinese Medicine; methanol and formic acid were chromatographically pure (US PA company), water was Milli-Q ultrapure water, and the rest of the reagents were analytically pure ( Beijing Chemical Factory).

[0026] UPLC-Triple-TOF / MS system: ExionLC TM AD type high performance liquid chromatography (AB SCIEX, USA), Triple TOF 5600 + Mass spectrometer equipped with ESI ion source (AB SCIEX, USA)

[0027] 2. Detection method of flavonoid chemical components in walnut flower:

[0028] 1. Chromatography and mass spectrometry conditions

[0029] Chromatographic conditions The column is ACQUITY BEH C 18 (100mm×2.1mm, 1.7μm); 0.1% formic acid-water as mobile phase A, methanol as mobile phase B, linear gradient elution, 0~1min, 20%~35%B; 1min~5min, 35% ~80% B; 5min~15min, 80%~100%B; 15min~17min, ...

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Abstract

The invention discloses a method for analyzing flavonoid chemical components in walnut flowers. The method comprises the following steps: extracting the medicine walnut flowers by using an ethanol heating reflux method, concentrating, redissolving, carrying out ultrasonic treatment, and filtering to obtain a test solution; detecting the test solution by using an ultrahigh performance liquid chromatography-quadrupole-time of flight mass spectrometry so as to obtain secondary mass spectrometry information of compounds, and analyzing the information about the chemical components in the detectionresult. The method provided by the invention can be used for rapidly and efficiently analyzing the flavonoid compounds in the walnut flowers, thus providing a basis for the research and rapid and accurate identification of effective substances of the walnut flowers and being beneficial to the development and utilization of the walnut flowers.

Description

technical field [0001] The invention relates to an analysis method of chemical components, in particular to an analysis method of flavonoid chemical components in walnut flowers based on ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry technology. Background technique [0002] Walnut flower is the style of walnut, also known as walnut button, longevity vegetable, asparagus. Folks eat it as a cold dish, and clinically make it into a tincture to remove warts. Its main components are linoleic acid glyceride, protein, carbohydrates and vitamins, etc. It has the functions of promoting human hematopoietic function, preventing arteriosclerosis, calming asthma and moistening intestines. At present, the research on the components of walnut flowers is mainly based on flavonoids, and there is no report on the analysis of flavonoids in walnut flowers using ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 马强闫萌萌孙永飞陈萌白桦
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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