PCR identifying method for nitrite bacteria

Abstract

The invention belongs to the technical field of bioengineering and provides a PCR identifying method for nitrite bacteria. A special feature gene-ammonia monooxygenase gene of nitrite bacteria is used as a template to design and synthesize a pair of specific primers; a nitrite bacteria genome DNA is used as a template for PCR amplification; the PCR amplification condition is optimized, and then the PCR amplification product is subjected to agarose level electrophoresis; and whether the bacterial strain belongs to nitrite bacteria or the nitrite bacteria is contained in the mixed flora can be judged according to the existence and size of the strip in a track lane. According to the method, the specific primer designed by taking the ammonia monooxygenase gene as a template is the PCR amplification primer; an experimental result is high in specificity; the experimental operation is simple; the result reliability is high; the identifying period of the nitrite bacteria is shortened in the manner of optimizing the PCR amplification condition; the amplification time is above 26% shorter than the conventional PCR amplification time; and a quick and effective identifying method is supplied for identifying the nitrite bacteria population.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and provides a PCR identification method for nitrosating bacteria. Background technique [0002] In recent years, my country's environmental pollution has become increasingly serious and has become a major problem facing mankind. In particular, ammonia nitrogen pollution in industrial and domestic sewage can cause a series of problems such as eutrophication of water bodies and disturbance of water body ecosystems. Biological nitrification denitrification is currently a hot spot in the field of water treatment. It is completed by nitrification and denitrification. Nitrification is divided into two stages. First, ammonia nitrogen is oxidized to nitrite nitrogen by nitrifying bacteria, and then nitrifying bacteria Oxidizes nitrite nitrogen to nitrate nitrogen. However, the compliance rate of biological nitrification denitrification pools that have been in operation is not high. The key to so...

AI Technical Summary

Problems solved by technology

This method uses general primers for the bacterial 16S rDNA region as amplification primers. After PCR amplification, the PCR products must be sequenced and compared before the bacteria can only be identified as genus. In this way, the results of PCR amplification using general primers are specific. It is not high, and it also prolongs the identification cycle of bacteria species, failing to achieve the purpose of rapid detection

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