33results about How to "Perfect theoretical basis" patented technology

The present invention provides a method for recovering the gas content of shale. The method restores the original gas content of mud shale through adsorption gas content and pore volume gas content, wherein the recovery of the adsorption gas content is based on Polanyi adsorption potential and London dispersion effect Potential energy theory and gas state equation are the theoretical basis, combined with the core column methane adsorption isotherm curve, gas component test data, shale porosity and water saturation, through the gas adsorption gas content between the formation temperature and 95 °C analysis process The change is obtained; the recovery of the pore volume and gas content is obtained through the state equation using the test data of porosity and water saturation. The theoretical basis of the gas content recovery method is more perfect, and it can restore the original content of each gas component in shale, which is conducive to more accurate, objective and comprehensive evaluation of the recoverability of shale gas reservoirs.

The invention provides a suspension type curtain pressure-bearing water foundation pit pump output determination method based on three-dimensional drawdown. Water level drawdown control value outsidea pit is firstly calculated, and the total water inflow control value is derived; after safe drawdown of the top of in-pit bearing water is determined, and the dewatering scheme is distributed, the foundation pit total water inflow mount and single well pump output of the dewatering scheme are determined through the safe drawdown control value of the in-pit bearing water, and then whether the foundation pit water inflow amount is in the control value or not can be further checked. According to the suspension type curtain pressure-bearing water foundation pit pump output determination method, based on the Darcy law and the flow equilibrium theorem, the three-dimensional seepage field formed by foundation pit dewatering below a suspension type water-stopping curtain and safe water level drawdown inside the pit and outside the pit are fully considered, the pump outputs of the foundation pit and a dewatering well under specific dewatering schemes can be precisely calculated, and it also can be guaranteed that the water level drawdown of the bearing water inside the pit and outside the pit according to the pump outputs are both in a safe control range. According to the constituted dewatering schemes based on the suspension type curtain pressure-bearing water foundation pit pump output determination method, the construction cost is lowered, and safety of dewatering construction is also ensured.

The invention provides a chiral drug mass spectrometry quantitative analysis method based on a chemical derivatization reaction and spectral deformation quantitative analysis theory, and the main process is as follows: 1) preparing a chiral acyl chloride probe capable of reacting with a chiral drug by an organic synthesis reaction; 2) using the chiral probe to react with the chiral drug to producea diastereomer complex product; 3) using mass spectrometry to determine the mass spectral data of each fragment ion of the diastereomer complex product; 4) using the spectral deformation quantitativeanalysis theory to extract quantitative information of a target components in the chiral drug from the mass spectral data of the diastereomer complex product. The chiral drug mass spectrometry quantitative analysis method is suitable for quantitative analysis of nitrogen, sulfur or phosphorus-containing chiral drugs without using a chromatography column, and provides a simple, low-cost, sensitiveand accurate chiral drug detection method in the medical field.

The invention discloses an exonuclease III aided target cycle amplification based unmarked DNA mass spectrum quantitative analysis method. According to the method, exonuclease III aided target cycle amplification strategy, mass spectrum and wave spectrum deformation quantitative theory model are combined together, so as to realize sensitive and accurate quantitative analysis of target DNA in a complicated biologicla sample. Meanwhile, the method overcomes disadvantages of existing methods for identifying single nucleotide polymorphism, and by utilizing the characteristic that mass spectrum hasa capacity of identifying different residual DNA segments, the strategy provided by the invention can clearly identify single nucleotide polymorphism.

The invention relates to a monohydric phenol urine test kit capable of resisting the interference of urine components and its preparation method, operation method and application, belonging to the field of biomedical testing. The test kit of the present invention is composed of a main bottle (ampule bottle) reagent and an auxiliary bottle (plastic bottle) reagent, wherein: the main bottle reagent is made of mercury, concentrated nitric acid, copper sulfate and distilled water as raw materials, and the auxiliary bottle The reagent consists of sodium nitrite and distilled water. The kit of the invention is prepared from mercury, concentrated nitric acid, copper sulfate and sodium nitrite through chemical reaction and solution. The urine test kit of the present invention has the function of resisting the interference of clinical urine sample components such as urea and protein, has high stability, short color reaction time, and high color sensitivity, which increases the applicability of the urine test reagent to various clinical urine samples , improve the validity and objectivity of the urine test, and can avoid the missed detection of clinical positive samples due to the interference of urine components urea and protein.

The present application relates to the detecting reagents and detection methods of porcine reproductive and respiratory virus. In one aspect, the present disclosure provides immunogenic fragments that encoding non-structural protein 2 (Nsp2). Particularly, the immunogenic fragments as provided herein are absent in an Nsp2 protein of an attenuated PRRSV but are present in an Nsp2 protein of a wild type PRRSV. The present disclosure also provides oligonucleotide primers that are useful in distinguishing an attenuated PRRSV from a wild type PRRSV. The present application further provides detection methods and detecting reagents for PRRSV, and also methods of distinguishing pigs immunized by an attenuated PRRSV from pigs infected with a wild type PRRSV.