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Universal surface-enhanced Raman spectrum quantitative analysis method

A surface-enhanced Raman, quantitative analysis technology, applied in the field of Raman spectroscopy instrument analysis and detection

Inactive Publication Date: 2014-04-16
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a universal surface-enhanced Raman spectroscopy quantitative analysis method in view of the deficiencies in the prior art. The multiplier effect of the sample SERS signal is effectively separated to solve the problem of accurate quantitative analysis of SERS spectra in complex chemical and biological systems

Method used

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  • Universal surface-enhanced Raman spectrum quantitative analysis method
  • Universal surface-enhanced Raman spectrum quantitative analysis method
  • Universal surface-enhanced Raman spectrum quantitative analysis method

Examples

Experimental program
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Effect test

Embodiment 1

[0060] Example 1: Using butylrhodamine B as the internal standard component to realize the SERS spectrum quantitative analysis of the concentration of rhodamine 6G in aqueous solution

[0061] In this embodiment, butylrhodamine B with a known concentration is added to the calibration sample and the sample to be tested as an internal standard component (see Figure 3 (a)) to achieve accurate SERS spectra of rhodamine 6G concentrations in aqueous solutions. Quantitative analysis, to verify the effectiveness of the novel SERS spectrum quantitative analysis model proposed by the present invention and the new strategy of SERS quantitative analysis.

[0062] Reagents and instruments: Silver nitrate, sodium citrate, and potassium chloride were purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd. Rhodamine 6G and butylrhodamine B were purchased from Aladdin Reagents (Shanghai) Co., Ltd. All reagents used in the experiment were of analytical grade, and all aqueous solutions were...

Embodiment 2

[0072] Example 2: Quantitative detection of the concentration of Rhodamine 6G in aqueous solution using nano-silver sol labeled with an appropriate amount of p-cresol as the SERS quantitative substrate

[0073] In this example, the nano-silver sol labeled p-cresol is used as the quantitative SERS substrate (see Figure 3 (b)) to realize the quantitative detection of the SERS spectrum of the concentration of rhodamine 6G in the aqueous solution to further illustrate the combination of the quantitative SERS substrate. The new SERS spectral quantitative analysis model proposed by the invention and the new strategy of SERS quantitative analysis have wider practicability and ease of use.

[0074] Reagents and instruments: p-cresylthiophenol was purchased from Aladdin Reagents (Shanghai) Co., Ltd. Other reagents and instrument parameters were set as in Example 1.

[0075] The main steps of this experiment are as follows:

[0076] 1) Synthesis of SERS quantitative substrate: take 26m...

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Abstract

The invention discloses a universal surface-enhanced Raman spectrum quantitative analysis method, comprising the following main contents of: 1) preparing an SERS (Surface Enhanced Raman Scattering) base with accurate quantitative function by marking the SERS base with proper amount of sulfhydryl compound; 2) performing SERS analysis detection on the to-be-measured substance by the quantitative SERS base, and building a novel SERS spectrum quantitative analysis model, namely a multiplier effect model; 3) using a special new SERS quantitative analysis strategy, namely, firstly estimating the multiplier effect of an SERS spectrum signal in a corrected sample, and eliminating the multiplier effect of the SERS spectrum signal in a to-be-measured sample through a 'dual-correction' strategy, thereby realizing accurate prediction on the concentration of the to-be-measured substance in the to-be-measured sample. The analysis method provided by the invention solves the problem of SERS spectrum accurate quantitative analysis of a complex chemical-biological system better.

Description

technical field [0001] The invention relates to the field of Raman spectroscopic instrument analysis and detection in chemical industry, food, environment, biology and other industries, in particular to a universal surface-enhanced Raman spectroscopic quantitative analysis method. Background technique [0002] Raman spectroscopy belongs to molecular vibration spectroscopy, which reflects the characteristic structure of molecules and can be used for the analysis and detection of samples including solids, liquids, gases, etc. However, the Raman scattering effect is a very weak process (generally only 10% of the incident light intensity -10 ), so its application is greatly limited. Fleischman et al. first obtained high-quality Raman spectra of monolayer pyridine molecules adsorbed on the surface of a rough silver electrode in 1974, thus discovering the surface-enhanced Raman spectroscopy effect. In the past thirty years, the invention of Surface Enhanced Raman Spectroscopy (S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 陈增萍金竞文俞汝勤
Owner HUNAN UNIV
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