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A monohydric phenolic substance urine test kit that resists the interference of urine components and its preparation method, operation method and application

A monohydric phenol and kit technology, applied in the field of biomedical testing, can solve the problems of missed testing, unstable reagents, and reduced clinical significance of urine testing, avoiding missed testing, reducing toxicity and pollution, and shortening color reaction time. Effect

Active Publication Date: 2021-09-21
武汉福朗科技发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, in addition to the influence of the above-mentioned interfering substances in the urine sample, a new problem of decomposition of the urine test reagent due to preparation and placement has also been added, that is, because the nitrous acid in it is extremely unstable, it can only exist in dilute solutions and is easy to decompose. The problem of unstable reagents
Therefore, there are obvious deficiencies in the current clinical urine test reagents. Due to the inability to resist the interference of urine components and the instability of the reagents, the clinical positive urine is missed, which greatly reduces the clinical significance of the urine test.

Method used

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  • A monohydric phenolic substance urine test kit that resists the interference of urine components and its preparation method, operation method and application
  • A monohydric phenolic substance urine test kit that resists the interference of urine components and its preparation method, operation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A monohydric phenol urine test kit that resists interference from urine components in this embodiment, the kit is composed of a main bottle (ampule bottle) reagent and a secondary bottle (plastic bottle) reagent;

[0039] Wherein: in parts by mass, the main bottle reagent is made from 1 part of mercury, 2.4 parts of concentrated nitric acid, 1.5 parts of copper sulfate pentahydrate copper sulfate and 13 parts of distilled water, and the auxiliary bottle reagent is made of 1 part of sodium nitrite and Composed of 6 parts of distilled water; the volume ratio of the main bottle reagent to the auxiliary bottle reagent is 1:0.5.

[0040] In this embodiment, the anti-interference of urine components mentioned above monohydric phenol urine test kit is prepared by the following method, and the steps are as follows:

[0041] (1) Add 1 part of mercury and 2.4 parts of concentrated nitric acid into a 150mL beaker, stir evenly, then heat up to 50°C and react for 50 minutes. At this...

Embodiment 2

[0045] A monohydric phenol urine test kit that resists interference from urine components in this embodiment, the kit is composed of a main bottle (ampule bottle) reagent and a secondary bottle (plastic bottle) reagent;

[0046] Wherein: in parts by mass, the main bottle reagent is made from 1 part of mercury, 3.0 parts of concentrated nitric acid, 1.5 parts of copper sulfate pentahydrate copper sulfate and 14 parts of distilled water, and the auxiliary bottle reagent is made of 1 part of sodium nitrite and Composed of 8 parts of distilled water; the volume ratio of the main bottle reagent to the auxiliary bottle reagent is 1:0.7.

[0047] In this embodiment, the anti-interference of urine components mentioned above monohydric phenol urine test kit is prepared by the following method, and the steps are as follows:

[0048] (1) Add 1 part of mercury and 3.0 parts of concentrated nitric acid into a 150mL beaker, stir evenly, then heat up to 40°C and react for 100min. At this tim...

Embodiment 3

[0052] A monohydric phenol urine test kit that resists interference from urine components in this embodiment, the kit is composed of a main bottle (ampule bottle) reagent and a secondary bottle (plastic bottle) reagent;

[0053] Wherein: in parts by mass, the main bottle reagent is made from 1 part of mercury, 3.2 parts of concentrated nitric acid, 1.5 parts of copper sulfate pentahydrate copper sulfate and 15 parts of distilled water, and the auxiliary bottle reagent is made of 1 part of sodium nitrite and Composed of 7 parts of distilled water; the volume ratio of the main bottle reagent to the auxiliary bottle reagent is 1:0.6.

[0054] In this embodiment, the anti-interference of urine components mentioned above monohydric phenol urine test kit is prepared by the following method, and the steps are as follows:

[0055] (1) Add 1 part of mercury and 3.2 parts of concentrated nitric acid into a 150mL beaker, stir evenly, then heat up to 35°C and react for 110min. At this tim...

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Abstract

The invention relates to a monohydric phenol urine test kit capable of resisting the interference of urine components and its preparation method, operation method and application, belonging to the field of biomedical testing. The test kit of the present invention is composed of a main bottle (ampule bottle) reagent and an auxiliary bottle (plastic bottle) reagent, wherein: the main bottle reagent is made of mercury, concentrated nitric acid, copper sulfate and distilled water as raw materials, and the auxiliary bottle The reagent consists of sodium nitrite and distilled water. The kit of the invention is prepared from mercury, concentrated nitric acid, copper sulfate and sodium nitrite through chemical reaction and solution. The urine test kit of the present invention has the function of resisting the interference of clinical urine sample components such as urea and protein, has high stability, short color reaction time, and high color sensitivity, which increases the applicability of the urine test reagent to various clinical urine samples , improve the validity and objectivity of the urine test, and can avoid the missed detection of clinical positive samples due to the interference of urine components urea and protein.

Description

technical field [0001] The invention belongs to the field of biomedical testing. More specifically, the invention relates to a urine test kit for monohydric phenols that resists the interference of urine components and its preparation method, operation method and application. Background technique [0002] Early diagnosis and early treatment of tumors are the key to improving the cure rate of tumors. Abnormal amino acid metabolism is common in patients with malignant tumors, that is, the process of protein decomposition into amino acids is enhanced, while the catabolism of amino acids is weakened, and excess and unusable amino acids are excreted. Abnormal amino acid metabolism exists in the early stage of tumorigenesis, and one of the important manifestations is the elevated content of p-hydroxyphenylalanine (tyrosine) in urine. The more active the metabolism of tumor cells, the more significant the abnormality of amino acid metabolism, and the more obvious the increase of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/82
CPCG01N21/78G01N21/82
Inventor 谭秋萍吴建国杨小钢刘卫程浩张文于国荣黄玉卉
Owner 武汉福朗科技发展有限公司
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