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Separating and identifying method for pig follicle-stimulating hormone beta sub-gene monomer

A technology of follicle-stimulating hormone and β subunit, which is applied in the field of isolation and identification of porcine follicle-stimulating hormone β-subunit gene haplotype, can solve unconfirmed, no follicle-stimulating hormone β-subunit gene haplotype, no follicle-stimulating hormone Hormone β subunit gene haplotype and reproductive traits

Inactive Publication Date: 2007-01-24
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

Linville et al. (2001) used the method of RFLP (restriction fragment length polymorphism) to find a polymorphism in the αTaqI restriction site of the gene, but did not confirm that this site can affect the reproductive performance of pig breeds
So far, there is no report on the haplotype of the follicle-stimulating hormone β subunit gene, and there is no research on the correlation between the haplotype of the follicle-stimulating hormone β subunit gene and reproductive traits

Method used

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Embodiment

[0022] 1) Collect pig ear tissues and use conventional phenol / chloroform extraction methods to extract genomic DNA. The DNA samples are diluted to 100ng / μL for use. There are 90 experimental pigs in total, from the Meishan Pig Conservation Center of Meishan Pig Breeding Center, Jiading District, Shanghai. Population.

[0023] 2) For the αTaq I single nucleotide polymorphism site C / T of the porcine follicle stimulating hormone β subunit gene, design specific primers for the follicle stimulating hormone β subunit gene, and perform PCR reaction to obtain the amplified product 1; primer Design and PCR reaction system refer to the method provided by Linville et al. (2001), using Primer Premier 5.0 software to design primers. Wherein, the gene-specific primer has the sequence of 5'-TTGCTGGAAAGCCATCTG-3' and 5'-GGTACTTTCACGGTCTCG-3', and the length of the amplified product 1 is 1765bp and contains the porcine follicle stimulating hormone β subunit gene αTaq I SNP site C / T.

[0024] 3) Ac...

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Abstract

The present invention relates to pig follicle stimulating hormone beta sub-gene haplotype separating and identifying method, and belongs to the field of livestock molecular biological technology. The method includes: extracting pig's genome DNA, PCR amplification of pig follicle stimulating hormone beta sub-gene, polymorphism detection of the amplified product, constitution of haplotype and pig's breeding character correlativity analysis. The present invention features the separated and identified new pig female hormone receptor gene haplotype H21. The new pig female hormone receptor gene haplotype H21 of the present invention is obviously correlative to pig's breeding character, and the present invention provides new molecular marking method for pig's marking assisted breeding and matching.

Description

Technical field [0001] The present invention relates to a method for isolating and identifying porcine follicle stimulating hormone β subunit gene haplotype. Through this method, a new porcine follicle stimulating hormone β subunit gene haplotype is constructed, using its significant correlation with pig reproductive traits Features, can provide a new molecular marker method for pig marker-assisted selection and selection. It belongs to the technical field of livestock molecular biology. Background technique [0002] The reproductive traits of pigs are related to the production level and economic benefits of the pig farm. According to Chris Haley, a well-known animal genetics and breeder, calculated in 1990, if the number of sows per litter increases by one, the pig industry in the United Kingdom can increase the net profit of 700 million pounds per year, while the entire former European Community can increase the net profit every year. Net profit of about 2 billion pounds. In my...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 潘玉春王起山李媛媛于丹丹李婧
Owner SHANGHAI JIAO TONG UNIV
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