Application of pig SNP (Single Nucleotide Polymorphism) molecular marker in screening of reproductive traits and breeding of pigs

A technique for molecular markers and pig reproduction, which is applied in the application field of pig SNP molecular markers in the screening of pig reproductive traits and pig breeding, and achieves the effect of simple and fast screening methods

Active Publication Date: 2020-11-27
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the influence of OLR1 gene on reproductive performance.

Method used

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  • Application of pig SNP (Single Nucleotide Polymorphism) molecular marker in screening of reproductive traits and breeding of pigs
  • Application of pig SNP (Single Nucleotide Polymorphism) molecular marker in screening of reproductive traits and breeding of pigs
  • Application of pig SNP (Single Nucleotide Polymorphism) molecular marker in screening of reproductive traits and breeding of pigs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Obtaining of swine OLR1 gene SNP detection fragment and establishment of polymorphic site detection method

[0020] 1. Extraction of pig genomic DNA

[0021] The test pig breed of the present invention is a black pig jointly bred by the Institute of Animal Husbandry and Veterinary Medicine of the Hubei Academy of Agricultural Sciences and Hubei Tianzhili High Quality Pig Breeding Co., Ltd. offspring. Pig genomic DNA was extracted using the Animal Tissue Genomic DNA Extraction Kit (PureLink TM Pro 96Genomic DNA Purification Kit, K182104A), the pig genome DNA was extracted according to the instructions of the kit. The concentration and quality of the extracted DNA were tested and stored at -20°C for future use.

[0022] 2. Obtaining the SNP genetic marker detection fragment of pig OLR1 gene

[0023] (1) PCR amplification

[0024] A pair of primers were designed according to the SNP genetic marker detection sequence (as shown in SEQ ID NO.1) in the genome s...

Embodiment 2

[0033] Example 2 Polymorphic distribution detection of molecular markers in black pigs

[0034] In this example, the polymorphism distribution law of the porcine OLR1 gene g.61808176 T>C site was detected in 355 black pigs, and the detection results are shown in Table 1.

[0035] Table 1 Distribution of polymorphisms at g.61808176 T>C site of OLR1 gene

[0036]

[0037] From the results in Table 1, it can be seen that in black pigs, the T>C site of OLR1 gene g.61808176 showed three genotypes: TT, TC and CC, among which there were more individuals with TT genotype, and the T allele frequency was 95.9%. The T allele is therefore the dominant allele.

Embodiment 3

[0038] Example 3 Association analysis between molecular markers and pig reproductive traits

[0039] In order to determine whether the porcine OLR1 gene g.61808176 T>C site is related to differences in pig reproductive traits, the method established in Example 1 was used for polymorphism detection, and the relationship between the different genotypes of this polymorphic site and the total litter size of pigs was analyzed Correlations between litter size and litter size. SAS statistical software (SAS Institute Inc, Version 9.1) GLM program was used to conduct variance analysis of different SNP genotype combinations, and a significance test was performed. The model used was:

[0040] Y ij =μ+G i +F j +e ij ;

[0041] Y ij is the trait phenotype value, μ is the average value, G i is genotype effect (including gene additive effect and dominant effect; additive effect uses 1, 0 and -1 represent TT, TC and CC genotype respectively, dominant effect uses 1, -1 and 1 represent T...

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Abstract

The invention discloses application of a pig SNP (Single Nucleotide Polymorphism) molecular marker in screening of reproductive traits and breeding of pigs, wherein the reproductive traits are total litter size traits and live litter size traits; the nucleotide sequence of the molecular marker is as shown in the sequence table SEQ ID NO.1; and T/C base mutation exists at the 70bp position of the sequence. The invention further provides a primer pair used for amplifying the molecular marker and a method for breeding by utilizing the molecular marker. The invention discovers that one SNP molecular marker in the pig OLR1 gene is related to the total litter size traits and the live litter size traits of pigs for the first time; therefore, the SNP molecular marker can be used for marker assisted selection breeding of pigs; namely, the invention provides new use of one SNP molecular marker in the pig OLR1 gene in marker assisted breeding of the reproductive traits of pigs; early screening ofthe reproductive traits of pigs is realized; and a screening method is simple and rapid.

Description

technical field [0001] The invention relates to the technical field of pig molecular markers, in particular to the application of a pig SNP molecular marker in the screening of pig reproductive traits and pig breeding. Background technique [0002] Among the reproductive traits of pigs, the traits of total litter size and live piglets are directly related to the economic benefits of the pig industry, and are an important economic trait. The overall economics of the pig industry are significant. However, due to the low heritability of pigs (around 0.1), progress in conventional selection for reproductive traits in sows has been slow. With the rise and rapid development of molecular biology techniques, the combination of conventional breeding and marker-assisted selection (MAS) can effectively accelerate the selection process of pig reproductive traits. Therefore, it is of great significance to find the key molecular genetic markers that control the reproductive traits of pi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 乔木武华玉彭先文周佳伟张宇吴俊静梅书棋徐忠孙华刘贵生董斌科宋忠旭赵海忠李良华李梓芃
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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