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Application of a pig SNP molecular marker in the screening of pig reproductive traits and pig breeding

A technology of molecular markers and traits, applied in the application field of pig SNP molecular markers in the screening of pig reproductive traits and pig breeding, to achieve the effect of simple and fast screening methods

Active Publication Date: 2022-05-17
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the influence of OLR1 gene on reproductive performance.

Method used

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  • Application of a pig SNP molecular marker in the screening of pig reproductive traits and pig breeding
  • Application of a pig SNP molecular marker in the screening of pig reproductive traits and pig breeding
  • Application of a pig SNP molecular marker in the screening of pig reproductive traits and pig breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Obtaining of swine OLR1 gene SNP detection fragment and establishment of polymorphic site detection method

[0020] 1. Extraction of pig genomic DNA

[0021] The test pig breed of the present invention is a black pig jointly bred by the Institute of Animal Husbandry and Veterinary Medicine of the Hubei Academy of Agricultural Sciences and Hubei Tianzhili High Quality Pig Breeding Co., Ltd. offspring. Pig genomic DNA was extracted using the Animal Tissue Genomic DNA Extraction Kit (PureLink TM Pro 96Genomic DNA Purification Kit, K182104A), the pig genome DNA was extracted according to the instructions of the kit. The concentration and quality of the extracted DNA were tested and stored at -20°C for future use.

[0022] 2. Obtaining the SNP genetic marker detection fragment of pig OLR1 gene

[0023] (1) PCR amplification

[0024] A pair of primers were designed according to the SNP genetic marker detection sequence (as shown in SEQ ID NO.1) in the genome s...

Embodiment 2

[0033] Example 2 Polymorphic distribution detection of molecular markers in black pigs

[0034] In this example, the polymorphism distribution law of the porcine OLR1 gene g.61808176 T>C site was detected in 355 black pigs, and the detection results are shown in Table 1.

[0035] Table 1 Distribution of polymorphisms at g.61808176 T>C site of OLR1 gene

[0036]

[0037] From the results in Table 1, it can be seen that in black pigs, the T>C site of OLR1 gene g.61808176 showed three genotypes: TT, TC and CC, among which there were more individuals with TT genotype, and the T allele frequency was 95.9%. The T allele is therefore the dominant allele.

Embodiment 3

[0038] Example 3 Association analysis between molecular markers and pig reproductive traits

[0039] In order to determine whether the porcine OLR1 gene g.61808176 T>C site is related to differences in pig reproductive traits, the method established in Example 1 was used for polymorphism detection, and the relationship between the different genotypes of this polymorphic site and the total litter size of pigs was analyzed Correlations between litter size and litter size. SAS statistical software (SAS Institute Inc, Version 9.1) GLM program was used to conduct variance analysis of different SNP genotype combinations, and a significance test was performed. The model used was:

[0040] Y ij =μ+G i +F j +e ij ;

[0041] Y ij is the trait phenotype value, μ is the average value, G i is genotype effect (including gene additive effect and dominant effect; additive effect uses 1, 0 and -1 represent TT, TC and CC genotype respectively, dominant effect uses 1, -1 and 1 represent T...

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Abstract

The invention discloses the application of a porcine SNP molecular marker in the screening of pig reproductive traits and pig breeding, wherein the reproductive traits are traits of total litter size and live litter size; the nucleotide sequence of the molecular marker is as follows: As shown in the list of SEQ ID NO.1, there is a T>C base mutation at the 70th bp of the sequence. The present invention also provides a pair of primers for amplifying the above-mentioned molecular markers and a breeding method using the above-mentioned molecular markers. The present invention discovers for the first time that a SNP molecular marker in the pig OLR1 gene is related to the traits of the total litter size and live piglet size of pigs, so it can be used in pig marker-assisted selection breeding, that is, a SNP molecular marker in the pig OLR1 gene is provided The new application in marker-assisted breeding of pig reproductive traits realizes the early screening of pig reproductive traits, and the screening method is simple and fast.

Description

technical field [0001] The invention relates to the technical field of pig molecular markers, in particular to the application of a pig SNP molecular marker in the screening of pig reproductive traits and pig breeding. Background technique [0002] Among the reproductive traits of pigs, the traits of total litter size and live piglets are directly related to the economic benefits of the pig industry, and are an important economic trait. The overall economics of the pig industry are significant. However, due to the low heritability of pigs (around 0.1), progress in conventional selection for reproductive traits in sows has been slow. With the rise and rapid development of molecular biology techniques, the combination of conventional breeding and marker-assisted selection (MAS) can effectively accelerate the selection process of pig reproductive traits. Therefore, it is of great significance to find the key molecular genetic markers that control the reproductive traits of pi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 乔木武华玉彭先文周佳伟张宇吴俊静梅书棋徐忠孙华刘贵生董斌科宋忠旭赵海忠李良华李梓芃
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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