Aided identification method of wheat with resistance to yellow dwarf and special primer pair thereof

A technology for auxiliary identification and yellow dwarf disease, applied in botany equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as resistance genes that have not been found, and achieve the effect of saving time and resources and high accuracy

Inactive Publication Date: 2012-12-05
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no truly effective resistance genes have been found in the primary gene pool of wheat

Method used

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  • Aided identification method of wheat with resistance to yellow dwarf and special primer pair thereof
  • Aided identification method of wheat with resistance to yellow dwarf and special primer pair thereof
  • Aided identification method of wheat with resistance to yellow dwarf and special primer pair thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, the discovery of primer pair

[0063] Sequences of 62 STK gene cDNA clones obtained by using a pair of primers designed from the cDNA sequence of an important yellow dwarf resistance gene (TiSTK1) to amplify the cDNA of anti-yellow dwarf material (Ti, YW642) and yellow dwarf susceptible wheat Through the determination and comparison analysis, it was found that there were 3 transcripts in T. intermedia, two of which were located on T. intermedia 7Ai#1L of the wheat 7D chromosome translocated to disease-resistant wheat, and this primer pair was translocated to the resistant wheat. Intermedium TiSTK1 gene sequence in diseased wheat, and highly homologous sequences in susceptible wheat, and found that there were two consecutive single nucleotide polymorphisms (SNPs) differences in the 5' non-coding region between the two, These two consecutive base differences were designed as the 3' end of the upstream primer TISTK1-ASF, and then the downstream primer TISTK1-...

Embodiment 2

[0066] Example 2, application of primers for auxiliary identification of yellow dwarf resistant wheat

Embodiment 3

[0086] Example 3, application of primer pairs for auxiliary identification of yellow dwarf resistant wheat

[0087] Apply the primer pair (primer pair consisting of TISTK1-ASF and TISTK1-ASR) designed in Example 1 to detect the following samples:

[0088] Disomic addition line L1 resistant to yellow dwarf disease; double-ended system 7Ai#1L resistant to yellow dwarf disease; translocation lines resistant to yellow dwarf disease: YW642 (HW642), YW443, YW243 and TC14; ); the wheat-Thinopyrum intermedia addition line Z1 (carrying Bdv4; resistant to yellow dwarf), PmV (carrying PmV; resistant to powdery mildew; sensitive to yellow dwarf) against yellow dwarf.

[0089] 1. Identification of disease resistance

[0090] Method is the same as step 1 of embodiment 2.

[0091] The results showed that: L1, 7Ai#1L, YW642(HW642), YW443, YW243, TC14 and Z1 showed high resistance to BYDV-GAV strain infection, and their severity to wheat yellow dwarf disease was 0, that is, they were all healt...

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Abstract

Provided are a botanical yellow dwarf disease resistant protein TiSTKI, derived from a yellow dwarf disease resistant material, and a coding gene thereof, for use in enhancing resistance against yellow dwarf disease in plants such as wheat. Also provided are an interfering RNA to inhibit TiSTKI gene expression and an application of such RNA in transgenic plant cultivation; a primer pair capable of expanding TiSTKI gene or a cDNA characteristic segment thereof, and an application of the primer pair in aiding to identify a yellow dwarf disease resistant plant.

Description

technical field [0001] The invention relates to a method for assisting identification of wheat resistant to yellow dwarf disease and a pair of special primers. Background technique [0002] Wheat yellow dwarf disease is a major viral disease of wheat caused by aphid-mediated barley yellow dwarf virus (BYDV). In recent years, due to changes in climatic conditions, wheat aphids that transmit barley yellow dwarf virus have occurred widely in my country and have become increasingly serious. Wheat yellow dwarf disease has a tendency to spread from the main areas such as Shanxi, Gansu, and Shaanxi to the Jianghuai and southwest wheat areas. . Therefore, breeding new wheat varieties resistant to yellow dwarf disease is the most economical and effective way to control the disease. [0003] Excellent and available disease resistance genes are the prerequisite for disease resistance breeding. So far, no effective resistance gene has been found in the wheat primary gene bank. Howeve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCA01H1/04C12N15/8283
Inventor 张增艳陈亮高兰英辛志勇
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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