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PRRSV and PCV2 co-infection mouse disease model

A disease model, mouse technology, applied in antiviral agents, biochemical equipment and methods, medical raw materials derived from viruses/phages, etc., can solve the problems of time-consuming, labor-intensive and inapplicable

Inactive Publication Date: 2010-04-07
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

SVN can only be said to be a better research method, which is very time-consuming and laborious, and is not suitable for daily PRRSV diagnosis. However, among all serological detection methods, this method is the only objective and reliable method for evaluating the immune protection level of pigs. method of letter

Method used

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  • PRRSV and PCV2 co-infection mouse disease model
  • PRRSV and PCV2 co-infection mouse disease model
  • PRRSV and PCV2 co-infection mouse disease model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0064] 1PCV2, PRRSV reproduction

[0065] (1) Reproduction of PCV2

[0066] When the PK15 cells in the culture flask are 40%-50% confluent, add 1 mL / bottle of virus solution, absorb for 1 hour, discard the virus solution, wash twice with PBS, add new 10% MEM medium, and incubate at 37°C. Cultivate in a 5% CO2 incubator, subculture when the cells cover the bottom of the bottle, and collect one bottle for each generation of cells for detection of cell proliferation, and use D-glucosamine treatment group as a control.

[0067] (2) Propagation of PRRSV

[0068] When the Marc-145 cells in the culture flask are 50%-60% confluent, add 1 mL / bottle of virus solution, absorb for 1 hour, discard the virus solution, wash twice with PBS, add new 10% MEM medium, and Cultivate in an incubator with 5% CO2 at 37°C, collect the cells when the bottom of the flask is covered, and freeze at -80°C for later use.

[0069]2 Preparation of pathological slides

[0070] (1) Production of slices

[...

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Abstract

The invention discloses a method for constructing a model animal, which is used in the research area of veterinary lemology, pathology and pharmacology and relates to non-pig pig breeding, respiratory syndromes and porcine circovirus 2 type diseases. The method is characterized in that in an economical common experimental animal, a typical pathologic change is generated in a mouse through a specific toxic material attacking method, the successful construction of a disease model is proved by adopting the methods for weight weighing, pathological section preparation, PCR detection and ELISA antibody detection, a further basis is provided for an in vivo experimental research correlative with the co-infection of two viruses, and a certain basis is established for a pharmacological experiment research. The disease model in the method is high in stability, simple and convenient in operation and practical in a wide range. Because a pig body experiment has a long period and a high cost, a feminine pig is difficult to screen, and more importantly, a disease cannot be copied in a pig body, the construction of a small animal infection model is very necessary for vaccine immune effect evaluations and virus pathogenic mechanism researches.

Description

technical field [0001] The present invention relates to a method for constructing a non-pig animal model of porcine reproductive and respiratory syndrome and porcine circular type 2 disease. The construction method is characterized in that, in an economical and commonly used experimental animal, mice are induced to produce typical lesions by a specific method, Different methods are used to detect the successful construction of disease models, laying the foundation for further clinical and pharmacological experimental research. Background technique [0002] 1 Pathogen Introduction [0003] Porcine reproductive and respiratory syndrome (PRRS), also known as porcine reproductive and respiratory syndrome, pig blue ear disease, so far, blue ear disease is still one of the most harmful diseases in today's pig industry. The harm of PRRS is not only manifested in the economic loss caused by the damage caused by the disease itself to pigs, but also because PRRS virus is the main key...

Claims

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Application Information

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IPC IPC(8): A61K35/76A61P31/14A61P31/20C12Q1/68G01N21/64G01N33/543A61K35/765
Inventor 吴国娟马元元张中文杨明郭玮
Owner BEIJING UNIV OF AGRI
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