Method for constructing membrane protein cDNA library and use thereof

A membrane protein and library technology, applied in the field of constructing membrane protein cDNA library, can solve the problems of membrane protein research interference, lack of high-throughput specific screening of membrane proteins, etc., and achieve the effect of improving screening efficiency and screening specificity
CN101338454BActive Publication Date: 2011-09-21IMMUNOPHARMACEUTIC INST OF HEFEI RUIDA CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
IMMUNOPHARMACEUTIC INST OF HEFEI RUIDA CO LTD
Publication Date
2011-09-21

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Abstract

The invention relates to a construction method of a Membrane protein cDNA library and an application thereof. The method constructs a eukaryotic expression vector used for the specificity selection of the membrane protein. The expression vector can perform clone recombination with any cDNA clone library provided with the recombination site attL of Lambada bacteriophage so as to acquire a cDNA expression library. After the transfection of COS-1 cell, expression membrane protein clone is further selected by immunofluorescence so as to acquire the membrane protein cDNA expression library. As theapplication of the method, the liver lymphocyte membrane protein cDNA library of a C57BL / 6 rat is built up. The method is proved to be an effective proposal of the high flux specificity selection membrane protein through the identification and analysis of the library, and the method provides a powerful method to describe the map of the membrane protein of the cell surface of certain issue and to research the unknown membrane protein.
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Description

technical field

[0001] The invention relates to a method for constructing a membrane protein cDNA library, including the construction of a eukaryotic expression vector for screening membrane proteins, the expression of membrane proteins on the surface of mammalian cell COS-1 and fluorescence screening; and applying it to mice Specific high-throughput screening of liver lymphocyte membrane proteins. Background technique

[0002] Membrane proteins are an important part of the plasma membrane and play a vital role in the biological functions of the plasma membrane. For example, transport proteins, connexins, and some enzymes play an important role in the material transport and exchange of cells, the formation and maintenance of cytoskeleton, and the generation of cellular energy and signal transduction. Among them, receptor protein, as a class of functionally diverse proteins, is essential in maintaining cell survival and development; and plays a decisive role in the process o...

Claims

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