Method for changing nitrogen utilization efficiency in plants

a technology of nitrogen utilization efficiency and plant, which is applied in the direction of plant/algae/fungi/lichens ingredients, peptide sources, peptide sources, etc., can solve the problems of nitrogen fertilizer being one of the most expensive nutrients to supply, and achieve the effects of enhancing plant growth, high or low expression, and changing nitrogen utilization efficiency

Inactive Publication Date: 2011-01-13
ACAD SINIC
View PDF1 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007]In one aspect, the present invention provides a method for changing nitrogen utilization efficiency in a plant comprising regulating the expression of Arabidopsis NRT1.7 or an orthologue thereof, so that the nitrogen remobilization from older leaves to young leaves in the plant is regulated, and thereby the nitrogen utilization efficiency is changed. According to an embodiment of the invention, a transgenic plant is prepared by transforming a plant with a construct for a high or low level of expression of NRT1.7. In one example of the invention, a transgenic plant having an enhanced plant growth is prepared by transforming a plant with an expression construct comprising a DNA sequence encoding Arabidopsis NRT1.7 or an orthologue thereof in a high level of expression, thereby the transgenic plant has an improved nitrate remobilization from older leaves to young leaves in the plant and nitrogen utilization efficiency. In another example of the invention, the transgenic plant having a retarded plant growth is prepared by transforming a plant with a construct for inhibiting the expression of NRT1.7 gene or orthologue thereof, whereby the transgenic plant has decreased nitrogen utilization efficiency.
[0008]In another aspect, the present invention provides a new chimera nitrate transporter of a NRT1.1 and NRT1.2, providing a high nitrate transport efficiency, wherein the chimera nitrate transporter has the amino acid sequence of SEQ ID NO: 11. In one embodiment of the invention, a transgenic plant having enhanced nitrogen utilization efficiency by transforming a plant w

Problems solved by technology

Nitrogen fertilizer is one of the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for changing nitrogen utilization efficiency in plants
  • Method for changing nitrogen utilization efficiency in plants
  • Method for changing nitrogen utilization efficiency in plants

Examples

Experimental program
Comparison scheme
Effect test

example 1

NRT1.7 Encodes a Low Affinity Nitrate Transporter

[0084]In Arabidopsis, there are 53 NRT1 (PTR) genes, some of which are known to transport nitrate, while others transport dipeptides. To determine the substrate specificity of NRT1.7, in vitro-synthesized cRNA was injected into Xenopus oocytes for electrophysiological analysis. After 2-day incubation in ND96, oocytes were voltage clamped at −60 mV, and then subjected to 300 ms voltage pulses from 0˜160 mV in −20 mV increments. NRT1.7-injected oocytes responded to 10 mM nitrate at pH 5.5 with inward currents. And the inward currents were elicited by nitrate but not by the dipeptides tested (FIG. 1A). The current elicited by nitrate was pH-dependent, with little or no current detected when exposed to nitrate at pH7.4. The pH dependence of the nitrate elicited currents suggested that NRT1.7 is a proton-coupled nitrate transporter.

[0085]Most of the nitrate transporters in NRT1 (PTR1) family function as a low-affinity transporter with exce...

example 2

NRT1.7 is Expressed in Phloem Tissue of Old Leaves

[0086]Microarray data from the public resource A. thaliana Expression Database CSB.DB shows that little or no expression of NRT1.7 can be detected in root, and that transcription levels in leaves increased as leaves age (FIG. 2). The differential expression of NRT1.7 in old and young leaves was further confirmed here by Western Blot analysis (FIG. 3A). Using BiP as loading control, the NRT1.7 protein level in the oldest leaves was about 25 times higher than that in the youngest leaves. In addition, the leaves were separated into distal lamina, proximal lamina, and central part including midrib and petiole for quantitative RT-PCR analysis. The NRT1.7 mRNA level was higher in the distal lamina of older leaves (FIG. 3B).

[0087]To determine where NRT1.7 is expressed, 13 independent transgenic lines expressing GUS driven by NRT1.7 promoter were analyzed. Consistent with Western Blot result, GUS staining was stronger in the older leaves, wh...

example 3

NRT1.7 is Localized to the Plasma Membrane

[0089]To investigate the subcellular localization of NRT1.7, green fluorescent protein (GFP) fused either N-terminally or C-terminally to NRT 1.7 was transiently expressed in Arabidopsis protoplasts under the control of the cauliflower mosaic virus 35S promoter. Green fluorescence was seen in cytoplasm in the GFP control, while the green fluorescence of NRT1.7-GFP and GFP-NRT1.7 (FIG. 5) was detected as a fine ring at the cell periphery, external to the chloroplasts, indicating that NRT1.7 is localized in the plasma membrane.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Electrical conductanceaaaaaaaaaa
Efficiencyaaaaaaaaaa
Login to view more

Abstract

The present invention provides a method for changing nitrogen utilization efficiency in a plant comprises regulating the expression of Arabidopsis NRT 1.7 or an orthologue thereof so that the nitrate remobilization from older leaves to young leaves in the plant is regulated, thereby the nitrogen utilization efficiency is changed. The present invention also provides a transgenic plant obtainable by transforming a plant with an expression construct with a high or low level of expression of NRT 1.7. On the other hand, the present invention yet provides a chimera nitrate transporter, a DNA molecule coding for this chimera transporter and an expression vector thereof.

Description

RELATED APPLICATIONS[0001]This application claims benefit of U.S. Provisional Application 61 / 223,744, filed on Jul. 8, 2009, which is incorporated by reference in its entirety herein.SUBMISSION OF SEQUENCE LISTING[0002]The Sequence Listing associated with this application is filed in electronic format via EFS-Web and hereby incorporated by reference into the specification in its entirety. The name of the text file containing the Sequence Listing is Sequence_List—16024—00012_US_ST25.txt. The size of the text file is 39 KB, and the text file was created on Jul. 8, 2010.FIELD OF THE INVENTION[0003]The present invention is related to a method for changing nitrogen utilization efficiency in a plant by regulating expression of a nitrate transporter.BACKGROUND OF THE INVENTION[0004]Nitrogen fertilizer is one of the most expensive nutrients to supply. 50-70% of the applied nitrogen is lost from the plant-soil system and causes water pollution (Peoples, 1995, in: P. E. Bacon, Editor, Nitroge...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H5/00C12N15/82C07K14/415C07H21/04
CPCC07K14/415C12N15/8243C12N15/8261Y02A40/146
Inventor TSAY, YI-FANGFAN, SHU-CHUNCHEN, HUI-YU
Owner ACAD SINIC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap