16 results about "Nitrate transporters" patented technology

The invention discloses a rice arbuscular mycorrhiza specifically induced nuclear factor YC subunit gene and application thereof. The nucleotide sequence of the rice nuclear factor YC subunit gene OsNF-YC3 is as shown in SEQ ID NO. 1. The amino acid sequence of the protein OsNF-YC3 coded by the gene OsNF-YC3 is as shown in SEQ ID NO. 2. The rice nitrate transporter gene OsNF-YC3 is applied to promotion of interaction of plants and arbuscular mycorrhizal fungi and improvement of phosphorus absorption in the symbiotic process of the plants.

The present invention belongs to the field of biotechnology, and specifically relates to a wheat low-affinity nitrate transporter gene. TaNPF7.6‑1A KASP markers and their applications. The present invention discovered a site polymorphism at chr1A:355628042 based on the Chinese Spring reference genome v1.0, with a T / G single nucleotide polymorphism. This site can serve as a molecular marker for the wheat low-affinity nitrate transporter gene. Based on this gene sequence information, the present invention developed a KASP molecular marker to detect the SNP polymorphism present in this gene. Specific primers for this KASP marker can effectively genotype germplasms with varying nitrogen efficiency, enabling molecular marker-assisted breeding of nitrogen-efficient germplasms and possessing excellent application value.

The invention belongs to the technical field of gene engineering, and particularly relates to a poa pratensis drought-resistant gene PpNRT2.4 and application thereof. The invention provides a nitrate transporter gene PpNRT2.4 from poa pratensis, provides a complete coding sequence of the nitrate transporter gene PpNRT2.4 and a protein sequence coded by the nitrate transporter gene PpNRT2.4, clarifies biological functions of the PpNRT2.4 gene, and verifies that the drought resistance of transgenic plants can be remarkably improved by overexpression of the PpNRT2.4 gene. A precise, efficient and safe innovative technical path is provided for creating new drought-resistant germplasm of turfgrass and other crops and breaking through the traditional breeding efficiency bottleneck.

The invention discloses an application of a tomato gene or a protein coded by the tomato gene in regulating and controlling the tolerance of plants to low nitrogen stress, and relates to the technical field of biology. The RBOH1 gene in the tomato is knocked out, compared with a wild plant, the nitrogen absorption capacity is reduced, the expression of nitrate transporter genes NRT1.1 and NRT2.1 is limited, under low nitrogen stress, the expression of the RBOH1 gene is increased by 3.2 times after treatment for one day, and is continuously and remarkably increased within 1-3 days, which indicates that the RBOH1 positively regulates the nitrogen absorption of the tomato.

The invention discloses a method for reducing perfluorooctanoic acid in crops by applying nitrate nitrogen fertilizer to competitively inhibit nitrate transporter protein, and relates to the technical field of agricultural pollutant control. Calcium nitrate Ca (NO3) 2 is used for providing 4-16 mmol / L or mmol / kg nitrate (NO3 <->) nitrogen fertilizer for lettuce planting under the water culture or soil culture condition; the transmembrane transport of nitrate transporter protein to perfluorooctanoic acid (PFOA) is competitively inhibited, and the accumulation amount of the edible part PFOA of the lettuce is reduced. By applying the nitrate nitrogen fertilizer, transmembrane absorption of NRT2.7 transport protein on PFOA is competitively inhibited, accumulation of PFOA in the lettuce is remarkably reduced, the food safety is improved, crop growth is synchronously promoted, and double benefits of pollution reduction and yield increase are achieved. The method is easy and convenient to operate, high in adaptability and suitable for water culture and soil culture conditions, and has the good popularization prospect and the cost advantage. The mechanism has universality in various crops and has wide extension application value.

This invention belongs to the field of biotechnology, and specifically discloses a... XstNPF6.2a Genes and their encoded proteins and silencing XstNPF6.2a Application of genes in eliminating the competitive advantage of Xanthium sibiricum. XstNPF6.2a The cDNA nucleotide sequence of the gene is shown in SEQ ID NO.1, and the sequence of the nitrate transporter it encodes is shown in SEQ ID NO.2. This invention achieves silencing in *Xanthium sibiricum*. XstNPF6.2a Genes that eliminate the competitive advantage of the invasive plant *Xanthium strumarium* over native plants, thus controlling its invasion. Silencing genes in *Xanthium strumarium*. XstNPF6.2a The gene-based approach involves using VIGS transient silencing technology to remove genes containing the gene shown in SEQ ID NO. 3. XstNPF6.2a pTRV2- gene silencing target sequence XstNPF6.2a The vector and pTRV1 vector were introduced into Xanthium sibiricum plants. XstNPF6.2a XstNPF6.2a After gene silencing, its expression level decreased by 58.1% compared with wild-type Xanthium strumarium plants, significantly reducing the nitrate nitrogen uptake rate, plant height, basal diameter, biomass, total nitrogen content, and total nitrogen accumulation of Xanthium strumarium, eliminating its growth advantage over the native plant Xanthium strumarium, and controlling its invasion.

The invention discloses a haynaldia villosa nitrate transporter protein HvNRT2-6V. 3 and an application of the haynaldia villosa nitrate transporter protein HvNRT2- The DNA (deoxyribonucleic acid) sequence of the HvNRT3-V is SEQ ID NO. 1, and the amino acid sequence coded by the HvNRT3-V is SEQ ID NO. 2 The gene is from Haynaldia villosa., an overexpression vector pLGY: HvNRT2-6V. 3 is transformed into an acceptor material Fielder by using an agrobacterium transgenic technology, and the obtained transgenic plant shows that the nitrogen assimilation ability of wheat can be effectively enhanced, so that the yield potential and the nitrogen utilization efficiency of the wheat are remarkably improved. The invention provides a new way for cultivating crops with high nitrogen fertilizer utilization efficiency.

The invention belongs to the field of biological gene engineering, and discloses a rape nitrate transporter gene BnaNRT1.1, the nucleotide sequence of which is shown as SEQ ID NO: 1, and a nitrate transporter obtained by encoding the rape nitrate transporter gene BnaNRT1.1. The invention also discloses application of the rape nitrate transporter gene BnaNRT1.1 or the nitrate transporter protein in regulation and control of rape growth, and an amplification primer for the rape nitrate transporter gene BnaNRT1.1. The invention also discloses application of the rape nitrate transporter gene BnaNRT1.1 or the nitrate transporter protein in regulation and control of rape growth. The oilseed rape nitrate transporter gene BnaNRT1.1 and the nitrate transporter protein obtained by encoding the oilseed rape nitrate transporter gene BnaNRT1.1 are overexpressed in oilseed rape, so that the nitrate absorption rate and biomass of the oilseed rape can be remarkably increased, and an effective method is provided for nitrogen fertilizer reduction and synergism in field production application; and a basis is provided for providing gene and germplasm resources for further improving the quality of the rape through genetic breeding.

To provide a new tomato mutant with an increased lycopene content without a decreased β-carotene content, and a method for producing the same. [Solution] We discovered that by introducing a mutation into the tomato nitrate transporter gene (NPF1 gene) and expressing a mutant protein with a large deletion in the C-terminal region, it is possible to increase the lycopene content in tomatoes without reducing the β-carotene content.

The invention provides a nitrate transporter gene and application thereof in regulating and controlling salt resistance of corn. It is found that ZmNRT1.1 A positively regulates the salt tolerance of corn, the salt stress sensitive phenotype of ZmNRT1.1 A is caused by Cl-stress, an important corn salt-resistant molecular mechanism is disclosed, and an important theoretical basis and gene resources are provided for corn salt-resistant improvement.

The application provides three editing targets of a corn nitrate transporter gene ZmNRT1.5, and the three editing targets can be used for editing the ZmNRT1.5 gene to obtain single or multiple base insertion, deletion or large fragment deletion and the like editing types, thereby providing genetic materials for in-depth analysis of the function of the gene.

The application discloses application of VvNRT1.1 and VvCNGC15 proteins in improving nitrogen utilization rate of grape, and belongs to the technical field of plant genetic engineering and molecular breeding. The application proves that the VvNRT1.1 and VvCNGC15 proteins directly physically interact, and overexpression of the VvNRT1.1 or VvCNGC15 can improve the absorption and assimilation efficiency of nitrate nitrogen of grape. Therefore, the VvNRT1.1 and VvCNGC15 can be used as key molecular targets for improving nitrogen utilization rate, and have potential application value.

In the present invention, it is found that the content of lycopene can be increased without decreasing the content of β-carotene in tomatoes by introducing a mutation into a nitrate transporter gene (NPF1 gene) of tomatoes to express a mutant protein in which a C-terminal region is largely deleted.

The present invention discloses a tobacco nitrate transporter NtNPF7.4 and its encoding gene, gene editing vector and application. The present invention cloned the encoding gene NtNPF7.4 gene of the tobacco nitrate transporter, and detected different tobacco tissues and organs by fluorescent quantitative PCR. The results showed that the gene was expressed in the roots, stems, leaves and flowers of tobacco. Subcellular localization analysis found that the NtNPF7.4 protein was located on the cell membrane, and the expression level of the NtNPF7.4 gene in the leaves was significantly increased after salt stress, indicating that the NtNPF7.4 protein was involved in the chloride ion transport process. The NtNPF7.4 gene was knocked out using a gene editing vector to construct a gene knockout strain. The chloride ion content in the roots and leaves of the knockout strain was significantly increased, which made it possible to cultivate tobacco enriched in chloride ions, providing a new direction for improving the low chloride content of flue-cured tobacco in my country.

This invention discloses the application of tomato genes or their encoded proteins in regulating plant tolerance to low nitrogen stress, relating to the field of biotechnology. By knocking out the RBOH1 gene in tomatoes, compared to wild-type plants, nitrogen uptake capacity was reduced, and the expression of nitrate transporter genes NRT1.1 and NRT2.1 was restricted. Under low nitrogen stress, RBOH1 gene expression was upregulated 3.2-fold after one day of treatment and maintained a sustained and significant increase for 1 to 3 days, demonstrating that RBOH1 positively regulates nitrogen uptake in tomatoes.