Nitrate transporter gene AtNRT1.1, coding protein and applications
A technology of nitrate transport, atnrt1.1, which is applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of nitrogen transport without growth and development
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 The present invention will be further described below in conjunction with experimental methods and data.
 1. Arabidopsis seedling culture:
 Wild-type and mutant seeds were sterilized by soaking in 0.5% sodium hypochlorite solution for 10 min, and the sterilized seeds were placed in a 4°C refrigerator for vernalization for 2 days, and then spread on MS (Murashige and Skoog) solid medium containing 1% agar. Germinate and grow in a light incubator at 22°C.
 2. Identification of homozygous mutants:
 DNA extraction: All experimental drugs used were purchased from Zhongke Ruitai Bioengineering Co., Ltd. Take about 0.5g fresh leaves of Arabidopsis thaliana, grind them into a paste, add 400μl SDS extract (0.5% SDS (W / V); 200mM Tris-Hcl, PH=8.0; 250mM NaCl; 25mM EDTA); 12,000r / min , centrifuged for 3min; take the supernatant, add an equal volume of isopropanol to shake gently, let it stand for 30min; 30 μl of ionized water, stored at 4°C for l...
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