Nitrate transporter gene AtNRT1.1, coding protein and applications

A technology of nitrate transport, atnrt1.1, which is applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of nitrogen transport without growth and development

Inactive Publication Date: 2014-03-19
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

But so far there is no report on the growth and development of the above-ground p

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  • Nitrate transporter gene AtNRT1.1, coding protein and applications
  • Nitrate transporter gene AtNRT1.1, coding protein and applications
  • Nitrate transporter gene AtNRT1.1, coding protein and applications

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Embodiment Construction

[0014] The present invention will be further described below in conjunction with experimental methods and data.

[0015] 1. Arabidopsis seedling culture:

[0016] Wild-type and mutant seeds were sterilized by soaking in 0.5% sodium hypochlorite solution for 10 min, and the sterilized seeds were placed in a 4°C refrigerator for vernalization for 2 days, and then spread on MS (Murashige and Skoog) solid medium containing 1% agar. Germinate and grow in a light incubator at 22°C.

[0017] 2. Identification of homozygous mutants:

[0018] DNA extraction: All experimental drugs used were purchased from Zhongke Ruitai Bioengineering Co., Ltd. Take about 0.5g fresh leaves of Arabidopsis thaliana, grind them into a paste, add 400μl SDS extract (0.5% SDS (W / V); 200mM Tris-Hcl, PH=8.0; 250mM NaCl; 25mM EDTA); 12,000r / min , centrifuged for 3min; take the supernatant, add an equal volume of isopropanol to shake gently, let it stand for 30min; 30 μl of ionized water, stored at 4°C for l...

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Abstract

The invention provides a nitrate transporter gene AtNRT1.1, a coding protein and applications, and belongs to the plant molecular biology and transgenosis related technology fields. Through insertion of arabidopsis AtNRT1.1 gene T-DNA into a homozygous strain of a mutant salk_138710, functions of the AtNRT1.1 gene at aspects of plant low-nitrogen tolerance and efficient utilization of nitrogen are researched. Experiment results show that, a AtNRT1.1 gene knockout mutant has greener leaf color than wild type and slightly longer roots and shows a low nitrogen tolerance phenotype without any addition of exogenous nitrogen element. Under treatment condition of else addition of 0.1 nM and 5 mM of ammonium nitrate, the nitrogen content of the mutant root is higher than that of the wide type but the nitrogen content of the overground part is obviously lower than that of the wide type (P is equal to 0.0001), which shows the AtNRT1.1 gene has important regulation and control effects on nitrogen element absorption and transportation under different concentrations, and predicts that the silencing of the gene can raise plant low-nitrogen tolerance ability and has great economic value in plant nitrogen nutrition efficient molecular breeding.

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Claims

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Application Information

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Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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