Plant root development related protein ZmNR1 and coding gene thereof

A technology that encodes genes and proteins, applied in the field of genetic engineering, can solve problems such as waste of resources, the effect of fertilization on food crops to increase production, the reduction of nutrient utilization efficiency of fertilizers, and ecological problems

Inactive Publication Date: 2011-04-06
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, with the gradual increase in the amount of chemical fertilizers used in food crops in my country, the effect of fertilization on food crops and the nutrient use efficiency of chemical fertilizers have been continuously reduced. This problem has become more significant after the 1990s.
For cereal crops, the average utilization rate of nitrogen fertilizer in the world

Method used

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  • Plant root development related protein ZmNR1 and coding gene thereof
  • Plant root development related protein ZmNR1 and coding gene thereof
  • Plant root development related protein ZmNR1 and coding gene thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1. Discovery and expression analysis of maize MADS-box transcription factor ZmNR1 gene

[0063] 1. Discovery of ZmNR1 gene

[0064] Using the strategy of homologous cloning, according to the reported model plant Arabidopsis thaliana that is involved in the regulation of lateral root elongation of the MADS-box transcription factor ANR1 (Zhang and Forde, 1998, Science, 279: 407-409) conserved domain M region The 80 amino acid sequence of, the homology comparison was carried out in the NCBI database, and the corn mRNA sequence EU976250, which was 96% similar to it, was obtained. The maize mRNA sequence EU976250 is the mRNA sequence of the maize MADS-box transcription factor ZmNR1. The mRNA sequence of ZmNR1 is composed of 681 bases, and its open reading frame (ORF) is from the 230-499th base of the 5'end, which encodes a MADS-box transcription factor protein of 89 amino acids with a molecular weight of 65KD, etc. Electricity point 8.15.

[0065] figure 1 Picture A is t...

Embodiment 2

[0101] Example 2. Cloning of ZmNR1 gene of maize and its application in promoting root development

[0102] 1. Cloning of the open reading frame sequence of maize ZmNR1 gene

[0103] 1. Extraction of total RNA from roots of maize inbred line B73 at seedling stage

[0104] Maize inbred line B73 (Maize inbred line B73 was cultivated by Iowa State University, USA. The B73 seeds used in this experiment were donated by Professor Schnable from Iowa State University, Schnable et al., The B73 Maize Genome: Complexity, Diversity, and Dynamics , Science 2009: 1112-1114, the public can obtain it from China Agricultural University). The total RNA of hydroponic root system at seedling stage was extracted with TRIzol Total RNA Extraction Kit (Takara Company), and the operation was performed according to the kit instructions. Specific steps are as follows:

[0105] Take 100mg of fresh corn root tissue and grind it in liquid nitrogen; add 1ml of TRIzol extract provided in the kit and shake at room ...

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Abstract

The invention discloses a plant root development related protein ZmNR1 and a coding gene thereof. The protein provided by the invention is a protein of (1) or (2) as follows: (1) a protein comprising amino acid sequences shown by the sequence 2 in a sequence table; and (2) a plant root development related protein derived from (1) by replacing and/or deleting and/or adding one or more amino acid residues to the amino acid residue sequence of the sequence 2 in the sequence table. The expression of the coding gene of the protein in arabidopsis can obviously increase the root size of Arabidopsis.An MADS-box transcription factor ZmNR1 gene regulated and controlled by nitrates and capable of controlling the growth and development of the plant root system is cloned from maize, a gene resource with more characteristics and effects is provided for researching root improvement and high-efficient nutrient absorption and utilization of main crops, and the protein plays an important role in the research of plant root improvement and high-efficient nutrient absorption and utilization in gene engineering.

Description

Technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a MADS-box transcription factor capable of improving plant roots and its coding genes and applications. Background technique [0002] Nitrogen is one of the essential mineral nutrients for plants, and it is also an important limiting factor for plant growth and development in agricultural production. It is closely related to crop yield and quality. my country is the largest producer and consumer of nitrogen fertilizer in the world. Extensive application of nitrogen fertilizer has made a huge contribution to the increase of crop production in my country. However, with the gradual increase in the amount of chemical fertilizers applied to grain crops in my country, the effect of increasing the yield of grain crops and the nutrient utilization efficiency of chemical fertilizers have been continuously reduced, and this problem has become more prominent after the 1990s. For ce...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 袁力行刘鹰米国华顾日良张福锁
Owner CHINA AGRI UNIV
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