Plant root development related protein ZmNR1 and coding gene thereof

A technology that encodes genes and proteins, applied in the field of genetic engineering, can solve problems such as the effect of fertilization on food crops to increase production, the reduction of nutrient utilization efficiency of chemical fertilizers, ecological problems, and environmental pollution

Inactive Publication Date: 2012-12-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the gradual increase in the amount of chemical fertilizers used in food crops in my country, the effect of fertilization on food crops and the nutrient use efficiency of chemical fertilizers have been continuously reduced. This problem has become more significant after the 1990s.
For cereal crops, the average utilization rate of nitrogen fertilizer in the world is only 33% (Raun and Johnson, 1999, Agron.J.91: 357-363). Nearly 70% of nitrogen in nitrogen fertilizer is lost through nitrate leaching and ammonia volatilization. The three major functions of denitrification and denitrification are lost from the soil, which not only causes a huge waste of resources, but also produces serious environmental pollution and ecological problems

Method used

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  • Plant root development related protein ZmNR1 and coding gene thereof
  • Plant root development related protein ZmNR1 and coding gene thereof
  • Plant root development related protein ZmNR1 and coding gene thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1. Discovery and expression analysis of maize MADS-box transcription factor ZmNR1 gene

[0064] 1. Discovery of the ZmNR1 gene

[0065] Using the homologous cloning strategy, according to the conserved domain M region of the MADS-box transcription factor ANR1 (Zhang and Forde, 1998, Science, 279:407-409) involved in the regulation of lateral root elongation in the model plant Arabidopsis The 80 amino acid sequences of the gene were homologously aligned in the NCBI database, and the maize mRNA sequence EU976250 with a similarity of 96% was obtained. Maize mRNA sequence EU976250 is the mRNA sequence of maize MADS-box transcription factor ZmNR1. The mRNA sequence of ZmNR1 consists of 681 bases, and its open reading frame (ORF) is 230-499 bases from the 5' end, encoding a MADS-box transcription factor protein containing 89 amino acids, and its molecular weight is 65KD, etc. Electric point 8.15.

[0066] figure 1 Figure A shows the phylogenetic analysis of ZmNR1....

Embodiment 2

[0102] Embodiment 2, cloning of maize ZmNR1 gene and its application in promoting root system development

[0103] 1. Cloning of the open reading frame sequence of the maize ZmNR1 gene

[0104] 1. Extract the extraction of total RNA from the root system of corn inbred line B73 seedling stage

[0105] Corn inbred line B73 (maize inbred line B73 was bred by Iowa State University, USA, and the B73 seeds used in this experiment were donated by Professor Schnable of Iowa State University, Schnable et al., The B73 Maize Genome: Complexity, Diversity, and Dynamics , Science 2009: 1112-1114, publicly available from China Agricultural University). The total RNA of the water culture root system at the seedling stage was extracted with the TRIzol Total RNA Extraction Kit (Takara Company), and the operation was performed according to the instructions of the kit. Specific steps are as follows:

[0106] Take 100mg of fresh corn root tissue and grind it in liquid nitrogen; add 1ml of the ...

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Abstract

The invention discloses a plant root development related protein ZmNR1 and a coding gene thereof. The protein provided by the invention is a protein of (1) or (2) as follows: (1) a protein comprising amino acid sequences shown by the sequence 2 in a sequence table; and (2) a plant root development related protein derived from (1) by replacing and / or deleting and / or adding one or more amino acid residues to the amino acid residue sequence of the sequence 2 in the sequence table. The expression of the coding gene of the protein in arabidopsis can obviously increase the root size of Arabidopsis. An MADS-box transcription factor ZmNR1 gene regulated and controlled by nitrates and capable of controlling the growth and development of the plant root system is cloned from maize, a gene resource with more characteristics and effects is provided for researching root improvement and high-efficient nutrient absorption and utilization of main crops, and the protein plays an important role in the research of plant root improvement and high-efficient nutrient absorption and utilization in gene engineering.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a MADS-box transcription factor capable of improving plant roots, its coding gene and its application. Background technique [0002] Nitrogen is one of the essential mineral nutrients for plants, and it is also an important limiting factor for plant growth and development in agricultural production. It is closely related to crop yield and quality. my country is the largest producer and consumer of nitrogen fertilizer in the world. A large amount of nitrogen fertilizer has made a huge contribution to the increase of crop yield in our country. However, with the gradual increase in the amount of chemical fertilizers used in food crops in my country, the yield-increasing effect of food crops and the nutrient use efficiency of chemical fertilizers have been continuously reduced, and this problem has become more significant after the 1990s. For cereal crops, the average u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H5/00
Inventor 袁力行刘鹰米国华顾日良张福锁
Owner CHINA AGRI UNIV
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