Panicle size controlling gene, mutant and application thereof

A rice and gene technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems affecting the potential mining and improvement of hybrid rice combination yield of rice varieties, single genetic background of rice varieties, and narrow sources.

Inactive Publication Date: 2013-08-14
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the dwarf genes currently used in rice production are all sd1, and the source is quite narrow
The single use of dwarf genes and the genetic recessiveness of dwarf genes lead to a single and narrow genetic background of rice varieties, which affects the further exploration and improvement of the yield potential of rice varieties, especially hybrid rice combinations.

Method used

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  • Panicle size controlling gene, mutant and application thereof
  • Panicle size controlling gene, mutant and application thereof
  • Panicle size controlling gene, mutant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1: Phenotypic analysis of mutant ssp1

[0073] Through genetic hybridization, near-isogenic lines: NP-SSP1 and NIL-ssp1 were constructed and obtained in Nipponbare (japonica) and Nanjing 6 (indica) backgrounds. Such as figure 1 As shown, the NIL-ssp1 plants under the genetic background of Nanjing 6 showed phenotypes such as semi-dwarf, smaller leaf angle, dark green leaves, and changed panicle shape.

[0074] NIL-ssp1 rice panicle becomes smaller ( figure 1 , B, C), incomplete heading ( figure 1 B). Statistics found that the number of grains per panicle of NIL-ssp1 ( figure 1 D), the number of primary branches ( figure 1 E), the number of secondary branches ( figure 1 F) are significantly less than NIL-SSP1. In particular, the reduction in the number of secondary branches in NIL-ssp1 rice was more significant, indicating that the decrease in the number of grains per panicle was mainly caused by the reduction in the number of secondary branches. In additi...

Embodiment 2

[0075] Example 2: The ssp1 gene inhibits cell division and cell elongation

[0076] Tissues from the same part of the last stalk of the near isogenic line were selected and fixed, and the transverse and longitudinal tissue sections were observed, and it was found that the last stalk of NIL-ssp1 ( image 3 A) The number of vascular bundles in the medium was significantly less than that of NP-SSP1 ( image 3 B), indicating that the cell division of NIL-ssp1 is inhibited; at the same time, the number and size of longitudinal cells in the last stem of NIL-ssp1 ( image 3 D) significantly smaller than NP-SSP1 ( image 3 C), indicating that both cell division and cell elongation are significantly inhibited in NIL-ssp1.

Embodiment 3

[0077] Example 3: Map-based cloning of the SSP1 gene

[0078] The F1 and F2 offspring obtained from crossing ssp1 with Nanjing No. 6 were used for genetic analysis. Statistical data and Chi-square test found that the segregation ratio of the trait was 3:1, indicating that the trait was controlled by a dominant single gene.

[0079] The phenotype separation of the F2 generation of the mapping population constructed by crossing ssp1 with Nanjing No. 6 was used to segregate SSP1 roughly between Marker 337 and Marker 481 on the long arm of chromosome 1. Then, the phenotype of the F2 generation single plant constructed by crossing ssp1 and Zhonghua 11 was used to isolate, and it was finely mapped to the 109kb region on BAC AP002910 ( Figure 4 ). This region contains 15 ORFs, 11 of which have predicted protein products. Further sequencing revealed that there were two point mutations in one of the genes: G-C at 1360bp at the C-terminus, leading to Gly-Arg, and A-C at 1366bp, leadi...

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PUM

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Abstract

The invention relates to a panicle size controlling gene SSP1 (Small and Sheathed Panicle 1) and a mutant gene ssp1 thereof, and action mechanism thereof in regulating plant height, leaf included angle and panicle size, and provides a gene sequence SEQ IDNO: 3 from the panicle size controlling gene mutant ssp1. The invention also relates to homologous genes of the panicle size controlling gene mutant ssp1 in barley, wheat, maize, corn, sorghum, soybean, cotton, rape, arabidopsis and other plants. The above mentioned genes are all referred to as panicle size controlling genes. The invention also relates to applications of the SSP1 gene and the mutant ssp1 gene in controlling plant height, improving lodging resistance, reducing leaf angle to improve photosynthetic efficiency, changing gibberellin response, suppressing panicle sprouting and breeding.

Description

technical field [0001] The present invention relates to control rice ear size gene SSP1 ( S mall and S heathed P anicle 1) and mutant ssp1 genes, and their mechanisms of action in regulating plant height, leaf angle, and panicle shape. The invention also relates to the application of the gene SSP1 for controlling the panicle size of rice and the mutant ssp1 gene in breeding aspects such as reducing plant height to improve lodging resistance, reducing leaf angle to improve photosynthetic efficiency, changing gibberellin response to suppress seed panicle germination, and the like. Background technique [0002] "Agriculture puts seeds first", improving yield has always been the main goal of crop genetics and breeding research. Rice plant type is composed of plant height, tiller number, tiller angle, leaf angle, and panicle type, and is also one of the important factors affecting rice yield. In the 1960s, dwarf breeding characterized by plant type improvement greatly increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/63C07K14/415A01H5/00C12N5/10C12N15/29
Inventor 傅向东刘正斌刘学英吴昆
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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