Culture medium using gamma-aminobutyric acid to promote hybrid liriodendron somatic embryogenesis and application thereof

A hybrid technology of Liriodendron tulipifera and GABA, which is applied in application, plant regeneration, horticulture, etc., to achieve the effects of improving efficiency, promoting the production of somatic embryos, and improving efficiency and quality

Active Publication Date: 2019-10-08
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, GABA has been proved to play a certain coordinating role between plant secondary growth and stress adaptation, but its role in promoting cell growth and differentiation and inducing somatic embryogenesis under the conditions of plant tissue or cell culture in vitro has rarely been reported.

Method used

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  • Culture medium using gamma-aminobutyric acid to promote hybrid liriodendron somatic embryogenesis and application thereof
  • Culture medium using gamma-aminobutyric acid to promote hybrid liriodendron somatic embryogenesis and application thereof
  • Culture medium using gamma-aminobutyric acid to promote hybrid liriodendron somatic embryogenesis and application thereof

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Experimental program
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Embodiment 1

[0029] 1) Somatic embryo induction medium:

[0030] 3 / 4MS+VC 5mg / L+sucrose 30g / L were used as the basic medium, and different concentrations of γ-aminobutyric acid were added to form the somatic embryo induction medium of hybrid Liriodendron chinensis. With the basic medium without γ-aminobutyric acid as the control, 6 concentration gradients (0mg / L, 2mg / L, 4mg / L, 6mg / L, 8mg / L, 10mg / L) were used, and each concentration was at least 3 dishes were inoculated, and 5 pieces of callus were inoculated in each dish, and the diameter of each piece of callus was about 0.5 cm. Cultured in the dark at 24°C, subcultured every 25 days.

[0031] The embryogenic callus was observed morphologically at different time points of somatic embryo induction, and the somatic embryos at each developmental stage were counted at 90 days after somatic embryo induction. mature somatic embryo. Among them, the total number of embryos is the embryoid body in the shape of spherical or ellipsoidal, heart-sh...

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Abstract

The invention discloses a culture medium using gamma-aminobutyric acid to promote hybrid liriodendron somatic embryogenesis and application thereof and belongs to the technical field of plant tissue culture. The culture medium is prepared by using 5mg/L of 3/4MC+VC and 30g/L sucrose as the basic culture medium and adding 0-10mg/L of gamma-aminobutyric acid, wherein the added gamma-aminobutyric acid can promote hybrid liriodendron callus somatic embryogenesis, accelerate somatic embryo development and increase somatic embryogenesis efficiency. When the culture medium is applied to hybrid liriodendron somatic embryogenesis and tissue culture, high somatic embryogenesis quantity and quality and fast somatic embryogenesis can be achieved, induced somatic embryo seedling number is extremely evidently higher than that of control, lateral root number and root length are evidently higher than those of the control, each callus can averagely form 20 regenerated plants, and the culture medium isquite high in practical application value.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium for promoting somatic embryogenesis of Liriodendron chinensis by using γ-aminobutyric acid and its application. Background technique [0002] Hybrid tulip tree (Liriodendron sino-americanum) has a beautiful tree shape and rapid growth, and is widely used in landscaping and fine wood processing. Nanjing Forestry University has established a stable and efficient hybrid Liriodendron somatic embryogenesis and rapid seedling growth system (Chen Jinhui et al., 2003), and realized large-scale application. Chen Jinhui et al. studied various factors affecting somatic embryogenesis of Liriodendron chinensis, mainly including genotype, explant, growth regulator ratio, light culture conditions, activated carbon, liquid suspension culture, etc. In addition, significant progress has been made in the changes of hormones, the expression and regulation o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈金慧陈婷婷施季森陆叶王丹丹陈新颖翁禹豪成铁龙
Owner NANJING FORESTRY UNIV
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