Culture medium using gamma-aminobutyric acid to promote hybrid liriodendron somatic embryogenesis and application thereof

A hybrid technology of Liriodendron tulipifera and GABA, which is applied in application, plant regeneration, horticulture, etc., to achieve the effects of improving efficiency, promoting the production of somatic embryos, and improving efficiency and quality
CN110301356AActive Publication Date: 2019-10-08NANJING FORESTRY UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING FORESTRY UNIV
Publication Date
2019-10-08

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Abstract

The invention discloses a culture medium using gamma-aminobutyric acid to promote hybrid liriodendron somatic embryogenesis and application thereof and belongs to the technical field of plant tissue culture. The culture medium is prepared by using 5mg / L of 3 / 4MC+VC and 30g / L sucrose as the basic culture medium and adding 0-10mg / L of gamma-aminobutyric acid, wherein the added gamma-aminobutyric acid can promote hybrid liriodendron callus somatic embryogenesis, accelerate somatic embryo development and increase somatic embryogenesis efficiency. When the culture medium is applied to hybrid liriodendron somatic embryogenesis and tissue culture, high somatic embryogenesis quantity and quality and fast somatic embryogenesis can be achieved, induced somatic embryo seedling number is extremely evidently higher than that of control, lateral root number and root length are evidently higher than those of the control, each callus can averagely form 20 regenerated plants, and the culture medium isquite high in practical application value.
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Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium for promoting somatic embryogenesis of Liriodendron chinensis by using γ-aminobutyric acid and its application. Background technique

[0002] Hybrid tulip tree (Liriodendron sino-americanum) has a beautiful tree shape and rapid growth, and is widely used in landscaping and fine wood processing. Nanjing Forestry University has established a stable and efficient hybrid Liriodendron somatic embryogenesis and rapid seedling growth system (Chen Jinhui et al., 2003), and realized large-scale application. Chen Jinhui et al. studied various factors affecting somatic embryogenesis of Liriodendron chinensis, mainly including genotype, explant, growth regulator ratio, light culture conditions, activated carbon, liquid suspension culture, etc. In addition, significant progress has been made in the changes of hormones, the expression and regulation o...

Claims

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