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Aureobasidium aureum MR-57 and application thereof

A technology of MR-57 and spores, applied in the field of MR-57 and its application field, can solve the problem of limited strain resources

Active Publication Date: 2022-03-18
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Pseudomonas, Trichoderma, Bacillus and Agrobacterium have been developed as bio-control bacteria and put into commercial production, but the strain resources are very limited. The control of bio-control bacteria is a research hotspot in recent years

Method used

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  • Aureobasidium aureum MR-57 and application thereof
  • Aureobasidium aureum MR-57 and application thereof
  • Aureobasidium aureum MR-57 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Screening, Identification and Preservation of Example 1 Bacterial Strain MR-57

[0039] 1. Screening

[0040] (1) The soil to be tested is 1-year-old rhizosphere soil, air-dried and passed through a 20-mesh sieve; weigh 10g of the sample in a 250mL Erlenmeyer flask containing 90mL of sterile saline and glass beads; take 1mL of the supernatant after fully shaking for 20min solution, add 9mL sterile saline to obtain a 10-fold dilution, and serially dilute to 10 -3 、10 -4 、10 -5 Standby; draw 200 μL dilutions of different gradient solutions and spread them on the plate containing PDA medium, and each treatment has 3 replicates; culture in the dark at 25°C for 7 days to select single colonies with different phenotypes and pass them for 3 times, and streak on The PDA medium was purified and cultured, and pure colonies were isolated and stored at 4°C.

[0041] (2) The bacterial strains obtained in the previous step were screened by plate confrontation culture method, and ...

Embodiment 2

[0055] The broad-spectrum antibacterial test of embodiment 2 bacterial strain MR-57

[0056] Rhizoctonia solani, Phytophthora cactorum, Botrytis cinerea, Fusarium oxysporum, Fusarium equiseti, Alternaria tenuissima, Alternaria liriodendra 9 pathogenic bacteria, Cylindrocarpon destructans, Mycocentrospora acerina, and Mycocentrospora acerina were used as target bacteria, and the antimicrobial spectrum test was carried out on the strain MR-57 by plate confrontation method. details as follows:

[0057] By confronting the culture method in the petri dish, get the test pathogenic bacteria bacterium cake (Rhizoctonia solani, Phytophthora cactorum, Botrytis cinerea, Fusarium oxysporum, Fusarium oxysporum, Fusarium equiseti, Alternaria tenuissima, Alternaria liriodendra, Cylindrocarpondestructans, Mycocentrospora acerina) and inoculate to the center of a plate containing PDA medium with a diameter of 90mm, At the same time, two bacterium cakes of strain MR-57 with a diameter of 8 mm...

Embodiment 3

[0063] Example 3 Bacterial strain MR-57 fermented liquid inhibits the growth of Fusarium equisetum mycelia

[0064] (1) Activation of test pathogenic bacteria and preparation of bacterial strain MR-57 fermentation broth

[0065] After placing Fusarium equiseti at room temperature for 30 minutes, pick the mycelia of Fusarium equiseti under aseptic conditions and inoculate them in the center of the plate containing PDA medium, and culture them upside down at 25°C. The filaments were subcultured to the 4th generation for later use.

[0066] Pick a single colony of strain MR-57 and put it into a 250mL Erlenmeyer flask containing 100mL of PDB culture solution, culture it with shaking at 25°C and 170r / min for 7 days, then filter it through 3 layers of gauze, and filter the obtained filtrate through a 0.22μm filter membrane to obtain Strain MR-57 sterile fermentation broth.

[0067](2) The bacterial strain MR-57 sterile fermented liquid is mixed with the PDA medium according to the...

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Abstract

The invention discloses an aureobasidium aureum MR-57 and application thereof, and belongs to the field of functional microorganism screening and application, the strain MR-57 is preserved in Guangdong Microbial Culture Collection Center on November 26, 2021, and the preservation number is GDMCC No: 62084. The bacterial strain MR-57 has relatively strong inhibition and antagonism effects on seven pathogenic bacteria such as column spore destroying bacteria and the like, and has relatively strong antagonism capability on liriodendron alternaria alternata, column spore destroying bacteria and mechanical bacteria echinacea; the sterile fermentation liquor of the strain MR-57 has an obvious inhibition effect on the growth of fusarium equisetum hyphae and can obviously reduce the spore germination rate of fusarium equisetum; a soil mixing method is used for verifying that the strain MR-57 has good colonization capability in soil; a potted plant biocontrol test proves that the strain MR-57 has a certain prevention and growth promoting effect on the divaricate saposhnikovia root rot, and the disease index of the divaricate saposhnikovia root rot can be obviously reduced.

Description

technical field [0001] The invention belongs to the technical field of functional microorganism screening and application, and in particular relates to a strain of Acrophialophora jodhpurensis MR-57 and its application. Background technique [0002] Fangfeng Saposhnikovia divaricata (Turcz.) Schisck. is a plant of the genus Fangfeng in the family Umbelliferae. It is used as medicine with the dried roots of plants without flowering stems; it is pungent, slightly sweet, and warm in nature. ; Mainly distributed in the three northeastern provinces, Hebei, Inner Mongolia and other places. In recent years, with the increase of the cultivation area of ​​Fangfeng, Fangfeng root rot has become more and more serious in the cultivation area. It mainly infects the stem base of Fangfeng and destroys the vascular bundles after the onset, resulting in wilting of leaves and dead plants, seriously affecting the yield and quality of Fangfeng. quality. Fusarium equisetia is an important path...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCA01N63/30
Inventor 王云贺韩忠明王娇王妍杨丽华孙卓韩梅杨利民
Owner JILIN AGRICULTURAL UNIV
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