A kind of method utilizing methyl jasmonate to carry out somatic embryogenesis of hybrid Liriodendron

A technology of methyl jasmonate and hybrid Liriodendron, which is applied in the field of tissue culture seedlings, can solve the problems of reducing the induction rate of somatic embryos and reducing the number of somatic embryos, so as to improve the generation efficiency of somatic embryos, reduce the rate of deformities, and improve the quality of somatic embryos. Effect on Maturity Rate

Active Publication Date: 2019-02-22
NANJING FORESTRY UNIV
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Problems solved by technology

Chen Xiaopeng found that high temperature can reduce the number of somatic embryos and reduce the induction rate of somatic embryos in the research of cucumber somatic embryogenesis

Method used

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  • A kind of method utilizing methyl jasmonate to carry out somatic embryogenesis of hybrid Liriodendron
  • A kind of method utilizing methyl jasmonate to carry out somatic embryogenesis of hybrid Liriodendron
  • A kind of method utilizing methyl jasmonate to carry out somatic embryogenesis of hybrid Liriodendron

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Experimental program
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Effect test

Embodiment 1

[0050] Induction of the embryogenic callus of the immature embryo of embodiment 1

[0051] 1. Seed sterilization

[0052] Before sterilization, the aggregated samara should be cut open to remove the seed wings, and the seeds should be washed with water for 30 minutes. Then carry out sterilization treatment in the ultra-clean workbench, put the seeds in a sterile triangular flask, add 75% ethanol, sterilize for 30 seconds, pour out the 75% ethanol and add 0.1% mercuric chloride, sterilize for 8-10 minutes, pour Remove the mercuric chloride solution, wash with sterile water 3 to 4 times, seal and store at 4°C after the treatment, and proceed to the next step of treatment.

[0053] 2. Induction of embryogenic callus

[0054] The seed coat was removed in a sterile environment, and immature embryos and endosperms were placed in 3 / 4MS medium supplemented with 2,4-D, BA and other hormones for callus induction culture. The culture conditions were 23°C, dark culture, The subculture ...

Embodiment 2

[0094] After a certain number of subcultures, the state of the callus was yellow and fine granule, the texture was firm, and the growth rate was relatively slow. At this time, the embryogenic callus of Liriodendron chinensis was in a better state. When the embryogenic callus grows and is in good condition, somatic embryo induction is carried out.

[0095] Directly use solid medium for somatic embryo induction, and put the callus in a good state directly on the somatic embryo induction medium for culture. Hybrid Liriodendron somatic embryo induction medium: 3 / 4MS+VC 5mg / L+ CH0.2g / L+activated carbon 2g / L+sucrose 40g / L+agar powder 8g / L, add MeJa and ABA (as shown in Table 3) of different concentrations in the culture medium, with not adding MeJa and ABA and only adding 2.0mg / L The medium of LABA was used as the control, and the effects of different concentrations of methyl jasmonate in the solid medium on the somatic embryogenesis of Liriodendron chinensis were observed.

[0096...

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Abstract

The invention discloses a method for somatic embryogenesis of Liriodendron chinensis by using methyl jasmonate, including seed sterilization, induction of embryogenic callus, establishment of embryogenic suspension cell lines, and acquisition and transition of liquid single cells . The step of inducing the occurrence of somatic embryos of Liriodendron chinensis, characterized in that, when inducing the somatic embryos of Liriodendron chinensis, methyl jasmonate and ABA are added to the culture medium, or methyl jasmonate is added separately. The method for utilizing methyl jasmonate of the present invention to carry out hybrid Liriodendron body rapid propagation, in the hybrid Liriodendron somatic embryogenesis process, adding plant growth regulator MeJa effectively improves somatic embryo induction rate, reduces deformity rate, and further It is very practical to improve the somatic embryogenesis efficiency of Liriodendron hybridum, and it is beneficial to the establishment of rapid propagation system of Liriodendron hybridum.

Description

technical field [0001] The invention belongs to the technical field of tissue culture seedlings, and in particular relates to a method for utilizing methyl jasmonate (MeJa) to perform somatic embryogenesis of a hybrid tulip tree. Background technique [0002] In recent years, people have continuously increased the research on plant somatic embryogenesis, which has achieved rapid development and achieved great results in decades. Because the somatic embryos induced by somatic cells have the characteristics of large number, fast growth, complete structure, and high reproductive efficiency, somatic embryogenesis technology has become an effective means for rapid plant reproduction, which is conducive to the preservation of excellent forest species and shortens the time for cultivating excellent varieties. Time, speeding up the development of industrialization, etc. are of great significance in the development of forestry. [0003] Plant somatic embryogenesis is an effective wa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 陈金慧孟岩施季森成铁龙杨立明张稼霁陆叶
Owner NANJING FORESTRY UNIV
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