Solid and liquid alternated culture inducement crossbreeding Chinese tulip tree liquid suspend cell embryo high-frequency generating and regenerating method

A technology of hybrid tulipwood and suspension cells, which is applied in plant regeneration, botany equipment and methods, plant cells, etc., can solve problems such as publication and isolation of a complete set of technology for hybrid tulipwood, and achieve improved regeneration efficiency and The effect of reproduction coefficient

Inactive Publication Date: 2008-01-30
NANJING FORESTRY UNIV
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Problems solved by technology

[0021]2. Physiological isolation: After the formation of somatic embryos, there is less contact with the vascular system of the mother plant or explant, that is, the phenomenon of so-called physiological isolation occurs
[0026] At present, in various documents on plant tissue culture technology, in addition to the report on the patent technology related to the tissue culture of Liriodendron tulipifera L. in the United States, Chen Jinhui induced the somatic cells of Liriodendron tulipifera L. through solid culture. In addition to reports on embryo related patented technologies, there has not been a complete set of technologies for inducing somatic embryogenesis by hybrid Liriodendron tulipifera suspension cells

Method used

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  • Solid and liquid alternated culture inducement crossbreeding Chinese tulip tree liquid suspend cell embryo high-frequency generating and regenerating method
  • Solid and liquid alternated culture inducement crossbreeding Chinese tulip tree liquid suspend cell embryo high-frequency generating and regenerating method
  • Solid and liquid alternated culture inducement crossbreeding Chinese tulip tree liquid suspend cell embryo high-frequency generating and regenerating method

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Embodiment Construction

[0073] Preparation method of immature embryos

[0074] Select excellent plants from the father and mother. From the end of April to the beginning of May, select the flower buds that are about to open with the corolla top slightly loosened, the pistil stigma covered with mucus and crystal bright spots on the mother plant in the full bloom, peel off the petals, and then use tweezers to clamp the stamens from the male flowers , Gently touch the pistil stigma from top to bottom until the stigma is covered with a layer of golden pollen, and finally close the petals. Two months after pollination (July midsummer time), aggregate samaras were collected.

[0075] The harvested polymeric samaras were kept at a low temperature at 4°C for 10 days, and the seed wings were cut and cut. Wash the seeds with detergent to remove the oil on the surface, rinse with tap water for 30 minutes, rinse with distilled water 3 times, 75% ethanol for 40 seconds, 0.1% mercuric chloride for 4 minutes, and steri...

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Abstract

The invention relates to a method for inducing a high-frequently generating of cross-breeding liriodendron liquid suspended somatic embryo by solid-liquid alternate culture and cross-breeding liriodendron regeneration. After an immature zygotic embryo from ball-shaped stage to cotyledon stage is obtained from separating, and goes through embryonic callus induction stage, embryonic callus propagation stage, the stage of the embryonic callus tissue suspension cell system establishing and pre-multiplication, embryonic suspension cell multiplication stage, the stage of stopping embryonic suspension cell multiplication and forming of embryo, somatic embryo development stage and somatic embryo plant regenerating stage, the regenerated plant of cross-breeding liriodendron can be obtained.

Description

1. Technical Field [0001] The invention belongs to the technical field of plant regeneration through tissue culture in forestry, and specifically relates to a solid-liquid alternate culture inducing high-frequency somatic embryo generation and plant regeneration technology of hybrid Liriodendron chinense liquid suspension cells. 2. Background technology [0002] Liriodendron, also known as mantlewood, duck foot wood, nine-layer bark, maple lotus, etc., is a plant of the genus Liriondendron of the Magno Liaceae (Magno Liaceae). There are only two species of this genus. One is the Chinese tulip tree (L.chinense[Hemsl.] Sarg, which is distributed in the central and northern subtropical regions of China (from Zhejiang and Fujian in the east to Yunnan and the border between China and Vietnam in the southwest). .), the other is Tulipifera (L.tulipifera L.) distributed in the eastern United States. Liriodendron plants are in a relatively primitive position in the classification of angio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 施季森龙伟陈金慧边黎明席梦利李火根
Owner NANJING FORESTRY UNIV
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