Rapid propagation method of liriodendron sino-americanum with leaves as explants

A technology of explants and mandarin jackets, which is applied in the field of woody plant tissue culture seedlings, can solve the problems of cuttings, grafting and propagation difficulties, and Asian and American mandarin jackets. high effect

Inactive Publication Date: 2019-08-16
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: In view of the above-mentioned problems existing in the prior art, the purpose of the present invention is to provide a rapid propagation me

Method used

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  • Rapid propagation method of liriodendron sino-americanum with leaves as explants
  • Rapid propagation method of liriodendron sino-americanum with leaves as explants
  • Rapid propagation method of liriodendron sino-americanum with leaves as explants

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Experimental program
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Effect test

Embodiment 1

[0055] Embodiment 1: the selection of disinfection time, explant, dark treatment time

[0056] Collection of explants: Choose a sunny day, around 2 pm, take the young leaves that germinate in spring, cut them off from the branches together with the petiole, mark them, put them in an ice box and bring them back to the laboratory, and place them in a refrigerator at 4°C overnight.

[0057] (1) Selection of disinfection time

[0058] Pretreatment and disinfection method of explants: Soak the leaves with petioles that have been placed overnight in detergent for 3-5 minutes, brush gently with a soft brush in one direction, rinse them, put them into the cup and seal them with gauze In a beaker, rinse under tap water for 30 minutes. Put the explants washed with running water into a pre-sterilized Erlenmeyer flask, shake and wash them twice with high-pressure sterilized cooled sterile water, then soak them in 75% alcohol for 30 seconds, and rinse them with sterile water after soaking...

Embodiment 2

[0076] Embodiment 2: the callus induction of Yameimandarin

[0077] Cut the leaves to an area of ​​1cm 2 Leaf disks of large and small sizes are inoculated in callus induction medium, and after about 7 days of cultivation, the edge of the leaf begins to curl; after about 20 days of cultivation, a small amount of transparent callus has begun to form at the incision of the edge of the leaf; after about 35 days of cultivation, the volume of the callus structure has formed on the entire leaf disk After that, the callus continued to proliferate, and the volume also continued to increase. After culturing for about 50 days, the callus structure was completely formed, showing a green, dense and solid shape with a grainy feel on the surface ( image 3 ).

[0078] (1) Effect of hormone ratio on XY1 callus induction

[0079] It can be seen from Table 2-1 that among the 9 treatments, treatment 6 has the highest callus induction rate for XY1, with an average value of 93.33%, and the high...

Embodiment 3

[0110] Embodiment 3: callus differentiation of S. japonica

[0111] Differentiation culture: In this example, MS was used as the basic medium, and three hormones of 6-BA, IBA, and KT were added in different concentrations, as well as 500 mg / L of CH, 30 g / L of sucrose, and 7.0 g / L of agar as the differentiation medium. The pH of the medium was adjusted to 5.72. In addition, 5.0g / L VC was added to different treatments. All media were sterilized and solidified in an autoclave at 121°C for 20 min before use. Orthogonal experiment L9 with 3 factors and 3 levels (3 3 ), each genotype was inoculated with 20 flasks under each treatment, and repeated 3 times.

[0112] Transfer the embryogenic callus to the differentiation medium, inoculate for about 25 days, the raised granules on the surface of the callus gradually turn into bud bumps, and turn from green to bright white; about 35 days after inoculation, the tender white bud bumps gradually emerge into light green about 50 days af...

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Abstract

The invention discloses a rapid propagation method of liriodendron sino-americanum with leaves as explants. The method comprises the steps of pretreatment and disinfection of leaves of liriodendron sino-americanum with three genotypes of XY1, XY2 and T8, callus induction medium, differentiatial medium, strong seedling medium, rooting medium and transplanting of hardening-seedlings. The method provided by the invention is suitable for the rapid propagation of liriodendron sino-americanum, and has the advantages of high propagation speed, large proliferation coefficient and high survival rate oftransplanting. Experiments verify that under the optimal culture conditions, the average induction rate of the highest callus induction of XY1 genotype is 93.33%, and the average proliferation coefficient of maximum callus differentiation is 7.10; the average number of leaves per plant in maximum strong seedling culture of XY2 genotype is as high as 9.4; the average rooting rate of the highest rooting culture of T8 genotype is 56.00%, and the survival rate of final hardening-seedling transplanting is 89%.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of woody plants, more specifically, to a method for rapid propagation of Chinese mandarin japonica with leaves as explants, and belongs to the technical field of tissue culture and seedling cultivation of woody plants. Background technique [0002] Liriodendron chinense (Hemsl.) Sarg.×Liriodendrontulipifera L.] was the first artificial cross between Liriodendron chinense (Hemsl.) Sarg.×Liriodendrontulipifera L. in 1963 by Professor Ye Peizhong of Nanjing Forestry University (L. tulipifera Linn.) and Liriodendron chinense (L.chinense sarg. ) obtained interspecific hybrids, which grow fast, have straight stems, bright colors, few diseases and insect pests, wide adaptability, obvious heterosis, high ornamental value, and are suitable for use as garden landscape and street tree species. Due to the poor reproductive ability of the Asian American mandarin tree itself, the natural seed setti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李火根宗亚仙朱腾飞涂忠华仲维平
Owner NANJING FORESTRY UNIV
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