Crossbred Chinese tulip tree clone trophozoite suspension cell line inducement method for the generating and regenerating of somatic embryo

A technology of hybrid Liriodendron and suspension cells, which is applied in plant regeneration, botany equipment and methods, plant cells, etc., can solve the problems of hybrid Liriodendron plant regeneration technology reports and isolation that have not yet been seen, and shorten the propagation time , overcoming the time limit, the effect of convenient material collection

Active Publication Date: 2008-01-30
NANJING FORESTRY UNIV
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Problems solved by technology

[0021]2. Physiological isolation: After the formation of somatic embryos, there is less contact with the vascular system of the mother plant or explant, that is, the phenomenon of so-called physiological isolation occurs
[0026] Among the various literatures on plant tissue culture technology at present, there is a report on the tissue culture related patent technology of Liriodendron tulipifera (Litiondendron tulipifera L.) in the United States, and Chen Jinhui used immature embryos to induce hybrid Liriodendron tulipifera L. through solid culture ( Liriondeadron hybrid) somatic embryo related patent technology reports, there is no report on hybrid tulip tulip adult tree vegetative body induced somatic embryogenesis and plant regeneration

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  • Crossbred Chinese tulip tree clone trophozoite suspension cell line inducement method for the generating and regenerating of somatic embryo
  • Crossbred Chinese tulip tree clone trophozoite suspension cell line inducement method for the generating and regenerating of somatic embryo

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Embodiment Construction

[0065] In spring, new shoots were collected from adult trees of hybrid Liriodendron tulipifera clones collected from the wild, and different parts of materials such as buds, stem tips, flower organs and new branches were taken, and they were cleaned with detergent for 10 minutes in the laboratory, and then rinsed with tap water for 2 hours. Soak in 75% alcohol for 30s on a clean bench, 0.1% HgCl 2 : Soak for 8-12min, rinse with sterile water for 5-6 times, blot the moisture on the surface of the material with sterile filter paper, put it into the callus induction medium MS medium, the additional concentration is 2.0mg / L 2,4-D, 0.2mg / L6-BA, agar and 5mg / L vitamin C, adjust pH to 5.7, dark culture temperature is 24-26℃;

[0066] Take the white callus induced by the above materials, callus with moist appearance and soft texture, and put it into MS liquid medium supplemented with 0.2mg / L KT, 0.2mg / L NAA, 0.2mg / L 2,4-D Carry out suspension culture in medium for 7-10 days, settle t...

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Abstract

The invention relates to a method for reducing somatic embryo generating and plant regenerating by clone vegetative suspension cell line of cross-bred liriodendron. The method adopts bud, stem tip, leaf, flower organ and new branch from different parts of a mother tree as induction materials for the generating of somatic embryo and young plant is obtained after the stage of callus induction, establishing and multiplication of suspension cell line, induction of suspension cell embryo development, maturity of embryo, somatic embryo germinating and plant regenerating. The method solves the problem of the somatic embryo generating and plant regenerating can not be reduced by adult cross-bred liriodendron vegetative directly, and provides the new way with short cycle, high propagation rate and low cost.

Description

1. Technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture in forestry, and in particular relates to a technique for inducing somatic embryogenesis and plant regeneration of a vegetative suspension cell line of hybrid Liriodendron tulipifera clone. 2. Background technology [0002] Liriodendron, also known as mandarin wood, duck foot wood, nine-layer bark, maple lotus, etc., is a plant of the genus Liriondendron in the Magnoliaceae (MagnoLiaceae). There are only two species of this genus, one is the Chinese tulipwood (L. .), and the other is Liriodendron tulipifera (L.tulipifera L.) distributed in the eastern United States. Liriodendron genus occupies a relatively primitive position in the taxonomic status of angiosperms. Although the distribution area is wide, it is mostly distributed in small groups at present. Liriodendron, which is distributed in China, has been listed as a national second-class rare and endange...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 施季森龙伟陈金慧边黎明席梦利
Owner NANJING FORESTRY UNIV
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